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Development and Standardization of Dot – ELISA for Detection of Neospora caninum Antibodies in Cattle and Comparison with Standard Indirect ELISA and Direct Agglutination Test (DAT)

BACKGROUND: Neospora caninum is a protozoan parasite from phylum apicomplexa and an important agent causing abortion in cattle which produce notable economic loss all around the world. METHODS: Dot-Elisa was set up performing checker board procedure and then 178 sera of cattle examined with commerci...

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Autores principales: HAMIDINEJAT, Hossein, HAJI HAJIKOLAEI, Mohamad Rahim, GHORBANPOOR, Masoud, NAMAVARI, Mehdi, GOL, Sara Mohamad Ali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4266130/
https://www.ncbi.nlm.nih.gov/pubmed/25516747
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author HAMIDINEJAT, Hossein
HAJI HAJIKOLAEI, Mohamad Rahim
GHORBANPOOR, Masoud
NAMAVARI, Mehdi
GOL, Sara Mohamad Ali
author_facet HAMIDINEJAT, Hossein
HAJI HAJIKOLAEI, Mohamad Rahim
GHORBANPOOR, Masoud
NAMAVARI, Mehdi
GOL, Sara Mohamad Ali
author_sort HAMIDINEJAT, Hossein
collection PubMed
description BACKGROUND: Neospora caninum is a protozoan parasite from phylum apicomplexa and an important agent causing abortion in cattle which produce notable economic loss all around the world. METHODS: Dot-Elisa was set up performing checker board procedure and then 178 sera of cattle examined with commercial indirect ELISA and direct agglutination test (DAT) were also evaluated by dot-ELISA afterwards. RESULTS: Kappa statistical analysis revealed that Dot-ELISA has good agreements with ELISA as well as the DAT and also, Mc Nemar’s analyzing showed that this procedure has acceptable ability to discriminate positive results. Relative sensitivity and specificity of Dot-ELISA were respectively 92.63% and 89.16% and 93.4% and 90.8% in comparison with ELISA and DAT. CONCLUSION: Since the dot-ELISA is easy, inexpensive and not needed high experience to interpret the results, it is superior to ELISA and DAT when we aim to screen the cattle on the farm and slaughterhouses or when the laboratory equipment is not available.
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spelling pubmed-42661302014-12-16 Development and Standardization of Dot – ELISA for Detection of Neospora caninum Antibodies in Cattle and Comparison with Standard Indirect ELISA and Direct Agglutination Test (DAT) HAMIDINEJAT, Hossein HAJI HAJIKOLAEI, Mohamad Rahim GHORBANPOOR, Masoud NAMAVARI, Mehdi GOL, Sara Mohamad Ali Iran J Parasitol Original Article BACKGROUND: Neospora caninum is a protozoan parasite from phylum apicomplexa and an important agent causing abortion in cattle which produce notable economic loss all around the world. METHODS: Dot-Elisa was set up performing checker board procedure and then 178 sera of cattle examined with commercial indirect ELISA and direct agglutination test (DAT) were also evaluated by dot-ELISA afterwards. RESULTS: Kappa statistical analysis revealed that Dot-ELISA has good agreements with ELISA as well as the DAT and also, Mc Nemar’s analyzing showed that this procedure has acceptable ability to discriminate positive results. Relative sensitivity and specificity of Dot-ELISA were respectively 92.63% and 89.16% and 93.4% and 90.8% in comparison with ELISA and DAT. CONCLUSION: Since the dot-ELISA is easy, inexpensive and not needed high experience to interpret the results, it is superior to ELISA and DAT when we aim to screen the cattle on the farm and slaughterhouses or when the laboratory equipment is not available. Tehran University of Medical Sciences 2013-10 /pmc/articles/PMC4266130/ /pubmed/25516747 Text en Copyright © Iranian Journal of Parasitology & Tehran University of Medical Sciences This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Original Article
HAMIDINEJAT, Hossein
HAJI HAJIKOLAEI, Mohamad Rahim
GHORBANPOOR, Masoud
NAMAVARI, Mehdi
GOL, Sara Mohamad Ali
Development and Standardization of Dot – ELISA for Detection of Neospora caninum Antibodies in Cattle and Comparison with Standard Indirect ELISA and Direct Agglutination Test (DAT)
title Development and Standardization of Dot – ELISA for Detection of Neospora caninum Antibodies in Cattle and Comparison with Standard Indirect ELISA and Direct Agglutination Test (DAT)
title_full Development and Standardization of Dot – ELISA for Detection of Neospora caninum Antibodies in Cattle and Comparison with Standard Indirect ELISA and Direct Agglutination Test (DAT)
title_fullStr Development and Standardization of Dot – ELISA for Detection of Neospora caninum Antibodies in Cattle and Comparison with Standard Indirect ELISA and Direct Agglutination Test (DAT)
title_full_unstemmed Development and Standardization of Dot – ELISA for Detection of Neospora caninum Antibodies in Cattle and Comparison with Standard Indirect ELISA and Direct Agglutination Test (DAT)
title_short Development and Standardization of Dot – ELISA for Detection of Neospora caninum Antibodies in Cattle and Comparison with Standard Indirect ELISA and Direct Agglutination Test (DAT)
title_sort development and standardization of dot – elisa for detection of neospora caninum antibodies in cattle and comparison with standard indirect elisa and direct agglutination test (dat)
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4266130/
https://www.ncbi.nlm.nih.gov/pubmed/25516747
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