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Multiplexed volumetric bar-chart chip for point-of-care diagnostics

Microfluidics have become an enabling technology for point-of-care and personalized diagnostics. Desirable capabilities of microfluidics-based diagnostic devices include simplicity, portability, low cost and the performance of multiplexed and quantitative measurements, ideally in a high-throughput f...

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Autores principales: Song, Yujun, Zhang, Yuanqing, Bernard, Paul E., Reuben, James M., Ueno, Naoto T., Arlinghaus, Ralph B., Zu, Youli, Qin, Lidong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4266136/
https://www.ncbi.nlm.nih.gov/pubmed/23250413
http://dx.doi.org/10.1038/ncomms2292
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author Song, Yujun
Zhang, Yuanqing
Bernard, Paul E.
Reuben, James M.
Ueno, Naoto T.
Arlinghaus, Ralph B.
Zu, Youli
Qin, Lidong
author_facet Song, Yujun
Zhang, Yuanqing
Bernard, Paul E.
Reuben, James M.
Ueno, Naoto T.
Arlinghaus, Ralph B.
Zu, Youli
Qin, Lidong
author_sort Song, Yujun
collection PubMed
description Microfluidics have become an enabling technology for point-of-care and personalized diagnostics. Desirable capabilities of microfluidics-based diagnostic devices include simplicity, portability, low cost and the performance of multiplexed and quantitative measurements, ideally in a high-throughput format. Here we present the multiplexed volumetric bar-chart chip (V-Chip), which integrates all these capabilities in one device. A key feature of the V-Chip is that quantitative results are displayed as bar charts directly on the device—without the need for optical instruments or any data processing or plotting steps. This is achieved by directly linking oxygen production by catalase, which is proportional to the concentration of the analyte, with the displacement of ink along channels on the device. We demonstrate the rapid quantification of protein biomarkers in diverse clinical samples with the V-Chip. The development of the V-Chip thus opens up the possibility of greatly simplified point-of-care and personalized diagnostics.
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spelling pubmed-42661362015-01-14 Multiplexed volumetric bar-chart chip for point-of-care diagnostics Song, Yujun Zhang, Yuanqing Bernard, Paul E. Reuben, James M. Ueno, Naoto T. Arlinghaus, Ralph B. Zu, Youli Qin, Lidong Nat Commun Article Microfluidics have become an enabling technology for point-of-care and personalized diagnostics. Desirable capabilities of microfluidics-based diagnostic devices include simplicity, portability, low cost and the performance of multiplexed and quantitative measurements, ideally in a high-throughput format. Here we present the multiplexed volumetric bar-chart chip (V-Chip), which integrates all these capabilities in one device. A key feature of the V-Chip is that quantitative results are displayed as bar charts directly on the device—without the need for optical instruments or any data processing or plotting steps. This is achieved by directly linking oxygen production by catalase, which is proportional to the concentration of the analyte, with the displacement of ink along channels on the device. We demonstrate the rapid quantification of protein biomarkers in diverse clinical samples with the V-Chip. The development of the V-Chip thus opens up the possibility of greatly simplified point-of-care and personalized diagnostics. Nature Pub. Group 2012-12-18 /pmc/articles/PMC4266136/ /pubmed/23250413 http://dx.doi.org/10.1038/ncomms2292 Text en Copyright © 2012, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Article
Song, Yujun
Zhang, Yuanqing
Bernard, Paul E.
Reuben, James M.
Ueno, Naoto T.
Arlinghaus, Ralph B.
Zu, Youli
Qin, Lidong
Multiplexed volumetric bar-chart chip for point-of-care diagnostics
title Multiplexed volumetric bar-chart chip for point-of-care diagnostics
title_full Multiplexed volumetric bar-chart chip for point-of-care diagnostics
title_fullStr Multiplexed volumetric bar-chart chip for point-of-care diagnostics
title_full_unstemmed Multiplexed volumetric bar-chart chip for point-of-care diagnostics
title_short Multiplexed volumetric bar-chart chip for point-of-care diagnostics
title_sort multiplexed volumetric bar-chart chip for point-of-care diagnostics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4266136/
https://www.ncbi.nlm.nih.gov/pubmed/23250413
http://dx.doi.org/10.1038/ncomms2292
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