GBF/Gea mutant with a single substitution sustains fungal growth in the absence of BIG/Sec7

Golgi Arf1-guanine nucleotide exchange factors (GEFs) belong to two subfamilies: GBF/Gea and BIG/Sec7. Both are conserved across eukaryotes, but the physiological role of each is not well understood. Aspergillus nidulans has a single member of the early Golgi GBF/Gea-subfamily, geaA, and the late Go...

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Autores principales: Arst, Herbert N., Hernandez-Gonzalez, Miguel, Peñalva, Miguel A., Pantazopoulou, Areti
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons Ltd 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4266534/
https://www.ncbi.nlm.nih.gov/pubmed/25451223
http://dx.doi.org/10.1016/j.febslet.2014.11.014
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author Arst, Herbert N.
Hernandez-Gonzalez, Miguel
Peñalva, Miguel A.
Pantazopoulou, Areti
author_facet Arst, Herbert N.
Hernandez-Gonzalez, Miguel
Peñalva, Miguel A.
Pantazopoulou, Areti
author_sort Arst, Herbert N.
collection PubMed
description Golgi Arf1-guanine nucleotide exchange factors (GEFs) belong to two subfamilies: GBF/Gea and BIG/Sec7. Both are conserved across eukaryotes, but the physiological role of each is not well understood. Aspergillus nidulans has a single member of the early Golgi GBF/Gea-subfamily, geaA, and the late Golgi BIG/Sec7-subfamily, hypB. Both geaA and hypB are essential. hypB5 conditionally blocks secretion. We sought extragenic hypB5 suppressors and obtained geaA1. geaA1 results in Tyr1022Cys within a conserved GBF/Gea-specific S(Y/W/F)(L/I) motif in GeaA. This mutation alters GeaA localization. Remarkably, geaA1 suppresses hypBΔ, indicating that a single mutant Golgi Arf1-GEF suffices for growth.
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spelling pubmed-42665342014-12-20 GBF/Gea mutant with a single substitution sustains fungal growth in the absence of BIG/Sec7 Arst, Herbert N. Hernandez-Gonzalez, Miguel Peñalva, Miguel A. Pantazopoulou, Areti FEBS Lett Article Golgi Arf1-guanine nucleotide exchange factors (GEFs) belong to two subfamilies: GBF/Gea and BIG/Sec7. Both are conserved across eukaryotes, but the physiological role of each is not well understood. Aspergillus nidulans has a single member of the early Golgi GBF/Gea-subfamily, geaA, and the late Golgi BIG/Sec7-subfamily, hypB. Both geaA and hypB are essential. hypB5 conditionally blocks secretion. We sought extragenic hypB5 suppressors and obtained geaA1. geaA1 results in Tyr1022Cys within a conserved GBF/Gea-specific S(Y/W/F)(L/I) motif in GeaA. This mutation alters GeaA localization. Remarkably, geaA1 suppresses hypBΔ, indicating that a single mutant Golgi Arf1-GEF suffices for growth. John Wiley & Sons Ltd 2014-12-20 /pmc/articles/PMC4266534/ /pubmed/25451223 http://dx.doi.org/10.1016/j.febslet.2014.11.014 Text en © 2014 The Authors https://creativecommons.org/licenses/by/3.0/This work is licensed under a Creative Commons Attribution 3.0 Unported License (https://creativecommons.org/licenses/by/3.0/) .
spellingShingle Article
Arst, Herbert N.
Hernandez-Gonzalez, Miguel
Peñalva, Miguel A.
Pantazopoulou, Areti
GBF/Gea mutant with a single substitution sustains fungal growth in the absence of BIG/Sec7
title GBF/Gea mutant with a single substitution sustains fungal growth in the absence of BIG/Sec7
title_full GBF/Gea mutant with a single substitution sustains fungal growth in the absence of BIG/Sec7
title_fullStr GBF/Gea mutant with a single substitution sustains fungal growth in the absence of BIG/Sec7
title_full_unstemmed GBF/Gea mutant with a single substitution sustains fungal growth in the absence of BIG/Sec7
title_short GBF/Gea mutant with a single substitution sustains fungal growth in the absence of BIG/Sec7
title_sort gbf/gea mutant with a single substitution sustains fungal growth in the absence of big/sec7
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4266534/
https://www.ncbi.nlm.nih.gov/pubmed/25451223
http://dx.doi.org/10.1016/j.febslet.2014.11.014
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