Cargando…

Epithelial Cells Are Active Participants in Vocal Fold Wound Healing: An In Vivo Animal Model of Injury

Vocal fold epithelial cells likely play an important, yet currently poorly defined, role in healing following injury, irritation and inflammation. In the present study, we sought to identify a possible role for growth factors, epidermal growth factor (EGF) and transforming growth factor-beta 1 (TGFβ...

Descripción completa

Detalles Bibliográficos
Autores principales: Leydon, Ciara, Imaizumi, Mitsuyoshi, Bartlett, Rebecca S., Wang, Sarah F., Thibeault, Susan L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4267843/
https://www.ncbi.nlm.nih.gov/pubmed/25514022
http://dx.doi.org/10.1371/journal.pone.0115389
_version_ 1782349205948334080
author Leydon, Ciara
Imaizumi, Mitsuyoshi
Bartlett, Rebecca S.
Wang, Sarah F.
Thibeault, Susan L.
author_facet Leydon, Ciara
Imaizumi, Mitsuyoshi
Bartlett, Rebecca S.
Wang, Sarah F.
Thibeault, Susan L.
author_sort Leydon, Ciara
collection PubMed
description Vocal fold epithelial cells likely play an important, yet currently poorly defined, role in healing following injury, irritation and inflammation. In the present study, we sought to identify a possible role for growth factors, epidermal growth factor (EGF) and transforming growth factor-beta 1 (TGFβ1), in epithelial regeneration during wound healing as a necessary first step for uncovering potential signaling mechanisms of vocal fold wound repair and remodeling. Using a rat model, we created unilateral vocal fold injuries and examined the timeline for epithelial healing and regeneration during early and late stages of wound healing using immunohistochemistry (IHC). We observed time-dependent secretion of the proliferation marker, ki67, growth factors EGF and TGFβ1, as well as activation of the EGF receptor (EGFR), in regenerating epithelium during the acute phase of injury. Ki67, growth factor, and EGFR expression peaked at day 3 post-injury. Presence of cytoplasmic and intercellular EGF and TGFβ1 staining occurred up to 5 days post-injury, consistent with a role for epithelial cells in synthesizing and secreting these growth factors. To confirm that epithelial cells contributed to the cytokine secretion, we examined epithelial cell growth factor secretion in vitro using polymerase chain reaction (PCR). Cultured pig vocal fold epithelial cells expressed both EGF and TGFβ1. Our in vivo and in vitro findings indicate that epithelial cells are active participants in the wound healing process. The exact mechanisms underlying their roles in autocrine and paracrine signaling guiding wound healing await study in a controlled, in vitro environment.
format Online
Article
Text
id pubmed-4267843
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-42678432014-12-26 Epithelial Cells Are Active Participants in Vocal Fold Wound Healing: An In Vivo Animal Model of Injury Leydon, Ciara Imaizumi, Mitsuyoshi Bartlett, Rebecca S. Wang, Sarah F. Thibeault, Susan L. PLoS One Research Article Vocal fold epithelial cells likely play an important, yet currently poorly defined, role in healing following injury, irritation and inflammation. In the present study, we sought to identify a possible role for growth factors, epidermal growth factor (EGF) and transforming growth factor-beta 1 (TGFβ1), in epithelial regeneration during wound healing as a necessary first step for uncovering potential signaling mechanisms of vocal fold wound repair and remodeling. Using a rat model, we created unilateral vocal fold injuries and examined the timeline for epithelial healing and regeneration during early and late stages of wound healing using immunohistochemistry (IHC). We observed time-dependent secretion of the proliferation marker, ki67, growth factors EGF and TGFβ1, as well as activation of the EGF receptor (EGFR), in regenerating epithelium during the acute phase of injury. Ki67, growth factor, and EGFR expression peaked at day 3 post-injury. Presence of cytoplasmic and intercellular EGF and TGFβ1 staining occurred up to 5 days post-injury, consistent with a role for epithelial cells in synthesizing and secreting these growth factors. To confirm that epithelial cells contributed to the cytokine secretion, we examined epithelial cell growth factor secretion in vitro using polymerase chain reaction (PCR). Cultured pig vocal fold epithelial cells expressed both EGF and TGFβ1. Our in vivo and in vitro findings indicate that epithelial cells are active participants in the wound healing process. The exact mechanisms underlying their roles in autocrine and paracrine signaling guiding wound healing await study in a controlled, in vitro environment. Public Library of Science 2014-12-16 /pmc/articles/PMC4267843/ /pubmed/25514022 http://dx.doi.org/10.1371/journal.pone.0115389 Text en © 2014 Leydon et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Leydon, Ciara
Imaizumi, Mitsuyoshi
Bartlett, Rebecca S.
Wang, Sarah F.
Thibeault, Susan L.
Epithelial Cells Are Active Participants in Vocal Fold Wound Healing: An In Vivo Animal Model of Injury
title Epithelial Cells Are Active Participants in Vocal Fold Wound Healing: An In Vivo Animal Model of Injury
title_full Epithelial Cells Are Active Participants in Vocal Fold Wound Healing: An In Vivo Animal Model of Injury
title_fullStr Epithelial Cells Are Active Participants in Vocal Fold Wound Healing: An In Vivo Animal Model of Injury
title_full_unstemmed Epithelial Cells Are Active Participants in Vocal Fold Wound Healing: An In Vivo Animal Model of Injury
title_short Epithelial Cells Are Active Participants in Vocal Fold Wound Healing: An In Vivo Animal Model of Injury
title_sort epithelial cells are active participants in vocal fold wound healing: an in vivo animal model of injury
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4267843/
https://www.ncbi.nlm.nih.gov/pubmed/25514022
http://dx.doi.org/10.1371/journal.pone.0115389
work_keys_str_mv AT leydonciara epithelialcellsareactiveparticipantsinvocalfoldwoundhealinganinvivoanimalmodelofinjury
AT imaizumimitsuyoshi epithelialcellsareactiveparticipantsinvocalfoldwoundhealinganinvivoanimalmodelofinjury
AT bartlettrebeccas epithelialcellsareactiveparticipantsinvocalfoldwoundhealinganinvivoanimalmodelofinjury
AT wangsarahf epithelialcellsareactiveparticipantsinvocalfoldwoundhealinganinvivoanimalmodelofinjury
AT thibeaultsusanl epithelialcellsareactiveparticipantsinvocalfoldwoundhealinganinvivoanimalmodelofinjury