Cargando…
Microbial Sensing by Goblet Cells Controls Immune Surveillance of Luminal Antigens in the Colon
The delivery of luminal substances across the intestinal epithelium to the immune system is a critical event in immune surveillance resulting in tolerance to dietary antigens and immunity to pathogens. How this process is regulated is largely unknown. Recently goblet cell associated passages (GAPs)...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4268115/ https://www.ncbi.nlm.nih.gov/pubmed/25005358 http://dx.doi.org/10.1038/mi.2014.58 |
_version_ | 1782349224269053952 |
---|---|
author | Knoop, Kathryn A McDonald, Keely G. McCrate, Stephanie McDole, Jeremiah R Newberry, Rodney D |
author_facet | Knoop, Kathryn A McDonald, Keely G. McCrate, Stephanie McDole, Jeremiah R Newberry, Rodney D |
author_sort | Knoop, Kathryn A |
collection | PubMed |
description | The delivery of luminal substances across the intestinal epithelium to the immune system is a critical event in immune surveillance resulting in tolerance to dietary antigens and immunity to pathogens. How this process is regulated is largely unknown. Recently goblet cell associated passages (GAPs) were identified as a pathway delivering luminal antigens to underlying lamina propria (LP) dendritic cells (DCs) in the steady state. Here we demonstrate that goblet cells (GCs) form GAPs in response to acetycholine (ACh) acting on muscarinic acetylcholine receptor (mAChR) 4. GAP formation in the small intestine (SI) was regulated at the level of ACh production, as GCs rapidly formed GAPs in response to ACh analogues. In contrast, colonic GAP formation was regulated at the level of GC responsiveness to ACh. Myd88 dependent microbial sensing by colonic GCs inhibited the ability of colonic GCs to respond to Ach to form GAPs and deliver luminal antigens to colonic LP-antigen presenting cells (APCs). Disruption of GC microbial sensing opened colonic GAPs and resulted in recruitment of neutrophils and APCs and production of inflammatory cytokines. Thus GC intrinsic sensing of the microbiota plays a critical role regulating the exposure of the colonic immune system to luminal substances. |
format | Online Article Text |
id | pubmed-4268115 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
record_format | MEDLINE/PubMed |
spelling | pubmed-42681152015-07-01 Microbial Sensing by Goblet Cells Controls Immune Surveillance of Luminal Antigens in the Colon Knoop, Kathryn A McDonald, Keely G. McCrate, Stephanie McDole, Jeremiah R Newberry, Rodney D Mucosal Immunol Article The delivery of luminal substances across the intestinal epithelium to the immune system is a critical event in immune surveillance resulting in tolerance to dietary antigens and immunity to pathogens. How this process is regulated is largely unknown. Recently goblet cell associated passages (GAPs) were identified as a pathway delivering luminal antigens to underlying lamina propria (LP) dendritic cells (DCs) in the steady state. Here we demonstrate that goblet cells (GCs) form GAPs in response to acetycholine (ACh) acting on muscarinic acetylcholine receptor (mAChR) 4. GAP formation in the small intestine (SI) was regulated at the level of ACh production, as GCs rapidly formed GAPs in response to ACh analogues. In contrast, colonic GAP formation was regulated at the level of GC responsiveness to ACh. Myd88 dependent microbial sensing by colonic GCs inhibited the ability of colonic GCs to respond to Ach to form GAPs and deliver luminal antigens to colonic LP-antigen presenting cells (APCs). Disruption of GC microbial sensing opened colonic GAPs and resulted in recruitment of neutrophils and APCs and production of inflammatory cytokines. Thus GC intrinsic sensing of the microbiota plays a critical role regulating the exposure of the colonic immune system to luminal substances. 2014-07-09 2015-01 /pmc/articles/PMC4268115/ /pubmed/25005358 http://dx.doi.org/10.1038/mi.2014.58 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Knoop, Kathryn A McDonald, Keely G. McCrate, Stephanie McDole, Jeremiah R Newberry, Rodney D Microbial Sensing by Goblet Cells Controls Immune Surveillance of Luminal Antigens in the Colon |
title | Microbial Sensing by Goblet Cells Controls Immune Surveillance of Luminal Antigens in the Colon |
title_full | Microbial Sensing by Goblet Cells Controls Immune Surveillance of Luminal Antigens in the Colon |
title_fullStr | Microbial Sensing by Goblet Cells Controls Immune Surveillance of Luminal Antigens in the Colon |
title_full_unstemmed | Microbial Sensing by Goblet Cells Controls Immune Surveillance of Luminal Antigens in the Colon |
title_short | Microbial Sensing by Goblet Cells Controls Immune Surveillance of Luminal Antigens in the Colon |
title_sort | microbial sensing by goblet cells controls immune surveillance of luminal antigens in the colon |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4268115/ https://www.ncbi.nlm.nih.gov/pubmed/25005358 http://dx.doi.org/10.1038/mi.2014.58 |
work_keys_str_mv | AT knoopkathryna microbialsensingbygobletcellscontrolsimmunesurveillanceofluminalantigensinthecolon AT mcdonaldkeelyg microbialsensingbygobletcellscontrolsimmunesurveillanceofluminalantigensinthecolon AT mccratestephanie microbialsensingbygobletcellscontrolsimmunesurveillanceofluminalantigensinthecolon AT mcdolejeremiahr microbialsensingbygobletcellscontrolsimmunesurveillanceofluminalantigensinthecolon AT newberryrodneyd microbialsensingbygobletcellscontrolsimmunesurveillanceofluminalantigensinthecolon |