Cargando…
Matrix metalloproteinase-13 downregulation and potential cartilage protective action of the Korean Red Ginseng preparation
BACKGROUND: The present study was designed to prepare and find the optimum active preparation or fraction from Korea Red Ginseng inhibiting matrix metalloproteinase-13 (MMP-13) expression, because MMP-13 is a pivotal enzyme to degrade the collagen matrix of the joint cartilage. METHODS: From total r...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4268559/ https://www.ncbi.nlm.nih.gov/pubmed/25535477 http://dx.doi.org/10.1016/j.jgr.2014.06.006 |
_version_ | 1782349252000743424 |
---|---|
author | Lee, Je Hyeong Shehzad, Omer Ko, Sung Kwon Kim, Yeong Shik Kim, Hyun Pyo |
author_facet | Lee, Je Hyeong Shehzad, Omer Ko, Sung Kwon Kim, Yeong Shik Kim, Hyun Pyo |
author_sort | Lee, Je Hyeong |
collection | PubMed |
description | BACKGROUND: The present study was designed to prepare and find the optimum active preparation or fraction from Korea Red Ginseng inhibiting matrix metalloproteinase-13 (MMP-13) expression, because MMP-13 is a pivotal enzyme to degrade the collagen matrix of the joint cartilage. METHODS: From total red ginseng ethanol extract, n-BuOH fraction (total ginsenoside-enriched fraction), ginsenoside diol-type-enriched fraction (GDF), and ginsenoside triol-type-enriched fraction (GTF) were prepared, and ginsenoside diol type-/F4-enriched fraction (GDF/F4) was obtained from Panax ginseng leaf extract. RESULTS: The n-BuOH fraction, GDF, and GDF/F4 clearly inhibited MMP-13 expression compared to interleukin-1β-treated SW1353 cells (human chondrosarcoma), whereas the total extract and ginsenoside diol-type-enriched fraction did not. In particular, GDF/F4, the most effective inhibitor, blocked the activation of p38 mitogen-activated protein kinase (p38 MAPK), c-Jun-activated protein kinase (JNK), and signal transducer and activator of transcription-1/2 (STAT-1/2) among the signal transcription pathways involved. Further, GDF/F4 also inhibited the glycosaminoglycan release from interleukin-1α-treated rabbit cartilage culture (30.6% inhibition at 30 μg/mL). CONCLUSION: Some preparations from Korean Red Ginseng and ginseng leaves, particularly GDF/F4, may possess the protective activity against cartilage degradation in joint disorders, and may have potential as new therapeutic agents. |
format | Online Article Text |
id | pubmed-4268559 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
record_format | MEDLINE/PubMed |
spelling | pubmed-42685592014-12-22 Matrix metalloproteinase-13 downregulation and potential cartilage protective action of the Korean Red Ginseng preparation Lee, Je Hyeong Shehzad, Omer Ko, Sung Kwon Kim, Yeong Shik Kim, Hyun Pyo J Ginseng Res Research Article BACKGROUND: The present study was designed to prepare and find the optimum active preparation or fraction from Korea Red Ginseng inhibiting matrix metalloproteinase-13 (MMP-13) expression, because MMP-13 is a pivotal enzyme to degrade the collagen matrix of the joint cartilage. METHODS: From total red ginseng ethanol extract, n-BuOH fraction (total ginsenoside-enriched fraction), ginsenoside diol-type-enriched fraction (GDF), and ginsenoside triol-type-enriched fraction (GTF) were prepared, and ginsenoside diol type-/F4-enriched fraction (GDF/F4) was obtained from Panax ginseng leaf extract. RESULTS: The n-BuOH fraction, GDF, and GDF/F4 clearly inhibited MMP-13 expression compared to interleukin-1β-treated SW1353 cells (human chondrosarcoma), whereas the total extract and ginsenoside diol-type-enriched fraction did not. In particular, GDF/F4, the most effective inhibitor, blocked the activation of p38 mitogen-activated protein kinase (p38 MAPK), c-Jun-activated protein kinase (JNK), and signal transducer and activator of transcription-1/2 (STAT-1/2) among the signal transcription pathways involved. Further, GDF/F4 also inhibited the glycosaminoglycan release from interleukin-1α-treated rabbit cartilage culture (30.6% inhibition at 30 μg/mL). CONCLUSION: Some preparations from Korean Red Ginseng and ginseng leaves, particularly GDF/F4, may possess the protective activity against cartilage degradation in joint disorders, and may have potential as new therapeutic agents. 2014-07-02 2015-01 /pmc/articles/PMC4268559/ /pubmed/25535477 http://dx.doi.org/10.1016/j.jgr.2014.06.006 Text en © 2015 The Korean Society of Ginseng. Published by Elsevier B.V. All rights reserved. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the CC-BY-NC License (http://creativecommons.org/licenses/by-nc/3.0). |
spellingShingle | Research Article Lee, Je Hyeong Shehzad, Omer Ko, Sung Kwon Kim, Yeong Shik Kim, Hyun Pyo Matrix metalloproteinase-13 downregulation and potential cartilage protective action of the Korean Red Ginseng preparation |
title | Matrix metalloproteinase-13 downregulation and potential cartilage protective action of the Korean Red Ginseng preparation |
title_full | Matrix metalloproteinase-13 downregulation and potential cartilage protective action of the Korean Red Ginseng preparation |
title_fullStr | Matrix metalloproteinase-13 downregulation and potential cartilage protective action of the Korean Red Ginseng preparation |
title_full_unstemmed | Matrix metalloproteinase-13 downregulation and potential cartilage protective action of the Korean Red Ginseng preparation |
title_short | Matrix metalloproteinase-13 downregulation and potential cartilage protective action of the Korean Red Ginseng preparation |
title_sort | matrix metalloproteinase-13 downregulation and potential cartilage protective action of the korean red ginseng preparation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4268559/ https://www.ncbi.nlm.nih.gov/pubmed/25535477 http://dx.doi.org/10.1016/j.jgr.2014.06.006 |
work_keys_str_mv | AT leejehyeong matrixmetalloproteinase13downregulationandpotentialcartilageprotectiveactionofthekoreanredginsengpreparation AT shehzadomer matrixmetalloproteinase13downregulationandpotentialcartilageprotectiveactionofthekoreanredginsengpreparation AT kosungkwon matrixmetalloproteinase13downregulationandpotentialcartilageprotectiveactionofthekoreanredginsengpreparation AT kimyeongshik matrixmetalloproteinase13downregulationandpotentialcartilageprotectiveactionofthekoreanredginsengpreparation AT kimhyunpyo matrixmetalloproteinase13downregulationandpotentialcartilageprotectiveactionofthekoreanredginsengpreparation |