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OM14 is a mitochondrial receptor for cytosolic ribosomes that supports co-translational import into mitochondria

It is well established that import of proteins into mitochondria can occur after their complete synthesis by cytosolic ribosomes. Recently, an additional model was revived, proposing that some proteins are imported co-translationally. This model entails association of ribosomes with the mitochondria...

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Autores principales: Lesnik, Chen, Cohen, Yifat, Atir-Lande, Avigail, Schuldiner, Maya, Arava, Yoav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4268710/
https://www.ncbi.nlm.nih.gov/pubmed/25487825
http://dx.doi.org/10.1038/ncomms6711
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author Lesnik, Chen
Cohen, Yifat
Atir-Lande, Avigail
Schuldiner, Maya
Arava, Yoav
author_facet Lesnik, Chen
Cohen, Yifat
Atir-Lande, Avigail
Schuldiner, Maya
Arava, Yoav
author_sort Lesnik, Chen
collection PubMed
description It is well established that import of proteins into mitochondria can occur after their complete synthesis by cytosolic ribosomes. Recently, an additional model was revived, proposing that some proteins are imported co-translationally. This model entails association of ribosomes with the mitochondrial outer membrane, shown to be mediated through the ribosome-associated chaperone nascent chain-associated complex (NAC). However, the mitochondrial receptor of this complex is unknown. Here, we identify the Saccharomyces cerevisiae outer membrane protein OM14 as a receptor for NAC. OM14Δ mitochondria have significantly lower amounts of associated NAC and ribosomes, and ribosomes from NAC[Δ] cells have reduced levels of associated OM14. Importantly, mitochondrial import assays reveal a significant decrease in import efficiency into OM14Δ mitochondria, and OM14-dependent import necessitates NAC. Our results identify OM14 as the first mitochondrial receptor for ribosome-associated NAC and reveal its importance for import. These results provide a strong support for an additional, co-translational mode of import into mitochondria.
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spelling pubmed-42687102014-12-29 OM14 is a mitochondrial receptor for cytosolic ribosomes that supports co-translational import into mitochondria Lesnik, Chen Cohen, Yifat Atir-Lande, Avigail Schuldiner, Maya Arava, Yoav Nat Commun Article It is well established that import of proteins into mitochondria can occur after their complete synthesis by cytosolic ribosomes. Recently, an additional model was revived, proposing that some proteins are imported co-translationally. This model entails association of ribosomes with the mitochondrial outer membrane, shown to be mediated through the ribosome-associated chaperone nascent chain-associated complex (NAC). However, the mitochondrial receptor of this complex is unknown. Here, we identify the Saccharomyces cerevisiae outer membrane protein OM14 as a receptor for NAC. OM14Δ mitochondria have significantly lower amounts of associated NAC and ribosomes, and ribosomes from NAC[Δ] cells have reduced levels of associated OM14. Importantly, mitochondrial import assays reveal a significant decrease in import efficiency into OM14Δ mitochondria, and OM14-dependent import necessitates NAC. Our results identify OM14 as the first mitochondrial receptor for ribosome-associated NAC and reveal its importance for import. These results provide a strong support for an additional, co-translational mode of import into mitochondria. Nature Pub. Group 2014-12-09 /pmc/articles/PMC4268710/ /pubmed/25487825 http://dx.doi.org/10.1038/ncomms6711 Text en Copyright © 2014, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Lesnik, Chen
Cohen, Yifat
Atir-Lande, Avigail
Schuldiner, Maya
Arava, Yoav
OM14 is a mitochondrial receptor for cytosolic ribosomes that supports co-translational import into mitochondria
title OM14 is a mitochondrial receptor for cytosolic ribosomes that supports co-translational import into mitochondria
title_full OM14 is a mitochondrial receptor for cytosolic ribosomes that supports co-translational import into mitochondria
title_fullStr OM14 is a mitochondrial receptor for cytosolic ribosomes that supports co-translational import into mitochondria
title_full_unstemmed OM14 is a mitochondrial receptor for cytosolic ribosomes that supports co-translational import into mitochondria
title_short OM14 is a mitochondrial receptor for cytosolic ribosomes that supports co-translational import into mitochondria
title_sort om14 is a mitochondrial receptor for cytosolic ribosomes that supports co-translational import into mitochondria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4268710/
https://www.ncbi.nlm.nih.gov/pubmed/25487825
http://dx.doi.org/10.1038/ncomms6711
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