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Brain cell apoptosis and enhancement of nervous excitability in pregnant rats with high plasma levels of homocysteine☆

Hyperhomocysteinemia is an important risk factor for preeclampsia-eclampsia. This study established a pregnant rat model of hyperhomocysteinemia, in which blood plasma homocysteine concentrations were twice or three times greater than that of normal pregnant rats. TUNEL revealed an increase in the n...

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Autores principales: Wang, Jun, Ge, Jing, Yang, Liu, Zhang, Haiyan, Li, Xuli, Xue, Dan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4268719/
https://www.ncbi.nlm.nih.gov/pubmed/25538740
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.028.005
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author Wang, Jun
Ge, Jing
Yang, Liu
Zhang, Haiyan
Li, Xuli
Xue, Dan
author_facet Wang, Jun
Ge, Jing
Yang, Liu
Zhang, Haiyan
Li, Xuli
Xue, Dan
author_sort Wang, Jun
collection PubMed
description Hyperhomocysteinemia is an important risk factor for preeclampsia-eclampsia. This study established a pregnant rat model of hyperhomocysteinemia, in which blood plasma homocysteine concentrations were twice or three times greater than that of normal pregnant rats. TUNEL revealed an increase in the number of apoptotic cells in the frontal cortex of pregnant rats with hyperhomocysteinemia. In addition, immunohistochemical staining detected activated nuclear factor-κB-positve cells in the frontal cortex. Reverse transcription-PCR detected that mRNA expression of the anti-apoptotic gene bcl-2 diminished in the frontal cortex. In situ hybridization and western blotting revealed that N-methyl-D-aspartate receptor 1 mRNA and protein expression was upregulated in the frontal cortex and hippocampus. These results indicate that hyperhomocysteinemia can induce brain cell apoptosis, increase nerve excitability, and promote the occurrence of preeclampsia in pregnant rats.
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spelling pubmed-42687192014-12-23 Brain cell apoptosis and enhancement of nervous excitability in pregnant rats with high plasma levels of homocysteine☆ Wang, Jun Ge, Jing Yang, Liu Zhang, Haiyan Li, Xuli Xue, Dan Neural Regen Res Research and Report Article: Brain Injury and Neural Regeneration Hyperhomocysteinemia is an important risk factor for preeclampsia-eclampsia. This study established a pregnant rat model of hyperhomocysteinemia, in which blood plasma homocysteine concentrations were twice or three times greater than that of normal pregnant rats. TUNEL revealed an increase in the number of apoptotic cells in the frontal cortex of pregnant rats with hyperhomocysteinemia. In addition, immunohistochemical staining detected activated nuclear factor-κB-positve cells in the frontal cortex. Reverse transcription-PCR detected that mRNA expression of the anti-apoptotic gene bcl-2 diminished in the frontal cortex. In situ hybridization and western blotting revealed that N-methyl-D-aspartate receptor 1 mRNA and protein expression was upregulated in the frontal cortex and hippocampus. These results indicate that hyperhomocysteinemia can induce brain cell apoptosis, increase nerve excitability, and promote the occurrence of preeclampsia in pregnant rats. Medknow Publications & Media Pvt Ltd 2012-10-05 /pmc/articles/PMC4268719/ /pubmed/25538740 http://dx.doi.org/10.3969/j.issn.1673-5374.2012.028.005 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research and Report Article: Brain Injury and Neural Regeneration
Wang, Jun
Ge, Jing
Yang, Liu
Zhang, Haiyan
Li, Xuli
Xue, Dan
Brain cell apoptosis and enhancement of nervous excitability in pregnant rats with high plasma levels of homocysteine☆
title Brain cell apoptosis and enhancement of nervous excitability in pregnant rats with high plasma levels of homocysteine☆
title_full Brain cell apoptosis and enhancement of nervous excitability in pregnant rats with high plasma levels of homocysteine☆
title_fullStr Brain cell apoptosis and enhancement of nervous excitability in pregnant rats with high plasma levels of homocysteine☆
title_full_unstemmed Brain cell apoptosis and enhancement of nervous excitability in pregnant rats with high plasma levels of homocysteine☆
title_short Brain cell apoptosis and enhancement of nervous excitability in pregnant rats with high plasma levels of homocysteine☆
title_sort brain cell apoptosis and enhancement of nervous excitability in pregnant rats with high plasma levels of homocysteine☆
topic Research and Report Article: Brain Injury and Neural Regeneration
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4268719/
https://www.ncbi.nlm.nih.gov/pubmed/25538740
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.028.005
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