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Expressions of apoptosis-related proteins in rats with focal cerebral ischemia after Angong Niuhuang sticker point application☆
In this study, we extracted and purified components in the Angong Niuhuang pill. Then we applied transdermal enhancers to Angong Niuhuang stickers by modern technology. The Angong Niuhuang sticker includes extracts from curcuma, berberine hydrochloride, baicalin, geniposide, borneol, and musk. Angon...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4268739/ https://www.ncbi.nlm.nih.gov/pubmed/25538759 http://dx.doi.org/10.3969/j.issn.1673-5374.2012.30.004 |
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author | Zhang, Dongshu Fu, Maodong Song, Chenglong Wang, Caiyun Lin, Xiaochun Liu, Yuanliang |
author_facet | Zhang, Dongshu Fu, Maodong Song, Chenglong Wang, Caiyun Lin, Xiaochun Liu, Yuanliang |
author_sort | Zhang, Dongshu |
collection | PubMed |
description | In this study, we extracted and purified components in the Angong Niuhuang pill. Then we applied transdermal enhancers to Angong Niuhuang stickers by modern technology. The Angong Niuhuang sticker includes extracts from curcuma, berberine hydrochloride, baicalin, geniposide, borneol, and musk. Angong Niuhuang stickers at different point application doses (1.35, 2.7, and 5.4 g/kg) were administered to Dazhui (DU14), Qihai (RN6) and Mingmen (DU4). Rats in the different dose point application and acupuncture groups were continuously administered for 7 days. Then a middle cerebral artery occlusion model was prepared for simulating human cerebral ischemia. Twelve hours later, expressions of Bcl-2, Bax and p53 protein in hippocampal CA1 were detected with immunohistochemistry. The expression level of Bcl-2, an anti-apoptotic protein, significantly increased in the high-, medium- and low-dose point application groups and the acupuncture group, while the expression of the pro-apoptotic proteins, Bax and p53, significantly decreased compared with the middle cerebral artery occlusion rats; and the Bcl-2/Bax ratio was significantly increased. The difference was noticeable for the high-dose point application group, which showed statistical difference compared with the low-dose point application group and the acupuncture group. Our experimental findings indicate that point application with Angong Niuhuang stickers promotes the expression of Bcl-2, and inhibits the expressions of Bax and p53 in the hippocampal CA1 area of rats after focal cerebral ischemia. Thus, point application of Angong Niuhang stickers protects brain tissues from cerebral ischemia. |
format | Online Article Text |
id | pubmed-4268739 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-42687392014-12-23 Expressions of apoptosis-related proteins in rats with focal cerebral ischemia after Angong Niuhuang sticker point application☆ Zhang, Dongshu Fu, Maodong Song, Chenglong Wang, Caiyun Lin, Xiaochun Liu, Yuanliang Neural Regen Res Research and Report: Traditional Chinese Medicine and Neural Regeneration In this study, we extracted and purified components in the Angong Niuhuang pill. Then we applied transdermal enhancers to Angong Niuhuang stickers by modern technology. The Angong Niuhuang sticker includes extracts from curcuma, berberine hydrochloride, baicalin, geniposide, borneol, and musk. Angong Niuhuang stickers at different point application doses (1.35, 2.7, and 5.4 g/kg) were administered to Dazhui (DU14), Qihai (RN6) and Mingmen (DU4). Rats in the different dose point application and acupuncture groups were continuously administered for 7 days. Then a middle cerebral artery occlusion model was prepared for simulating human cerebral ischemia. Twelve hours later, expressions of Bcl-2, Bax and p53 protein in hippocampal CA1 were detected with immunohistochemistry. The expression level of Bcl-2, an anti-apoptotic protein, significantly increased in the high-, medium- and low-dose point application groups and the acupuncture group, while the expression of the pro-apoptotic proteins, Bax and p53, significantly decreased compared with the middle cerebral artery occlusion rats; and the Bcl-2/Bax ratio was significantly increased. The difference was noticeable for the high-dose point application group, which showed statistical difference compared with the low-dose point application group and the acupuncture group. Our experimental findings indicate that point application with Angong Niuhuang stickers promotes the expression of Bcl-2, and inhibits the expressions of Bax and p53 in the hippocampal CA1 area of rats after focal cerebral ischemia. Thus, point application of Angong Niuhang stickers protects brain tissues from cerebral ischemia. Medknow Publications & Media Pvt Ltd 2012-10-25 /pmc/articles/PMC4268739/ /pubmed/25538759 http://dx.doi.org/10.3969/j.issn.1673-5374.2012.30.004 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research and Report: Traditional Chinese Medicine and Neural Regeneration Zhang, Dongshu Fu, Maodong Song, Chenglong Wang, Caiyun Lin, Xiaochun Liu, Yuanliang Expressions of apoptosis-related proteins in rats with focal cerebral ischemia after Angong Niuhuang sticker point application☆ |
title | Expressions of apoptosis-related proteins in rats with focal cerebral ischemia after Angong Niuhuang sticker point application☆ |
title_full | Expressions of apoptosis-related proteins in rats with focal cerebral ischemia after Angong Niuhuang sticker point application☆ |
title_fullStr | Expressions of apoptosis-related proteins in rats with focal cerebral ischemia after Angong Niuhuang sticker point application☆ |
title_full_unstemmed | Expressions of apoptosis-related proteins in rats with focal cerebral ischemia after Angong Niuhuang sticker point application☆ |
title_short | Expressions of apoptosis-related proteins in rats with focal cerebral ischemia after Angong Niuhuang sticker point application☆ |
title_sort | expressions of apoptosis-related proteins in rats with focal cerebral ischemia after angong niuhuang sticker point application☆ |
topic | Research and Report: Traditional Chinese Medicine and Neural Regeneration |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4268739/ https://www.ncbi.nlm.nih.gov/pubmed/25538759 http://dx.doi.org/10.3969/j.issn.1673-5374.2012.30.004 |
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