Optimized DPPH assay in a detergent-based buffer system for measuring antioxidant activity of proteins

The free radical method using 1,1-diphenyl-2-picryl-hydrazyl (DPPH) is a well established assay for the in vitro • Non-ionic detergents were added to keep the DPPH radical soluble and to provide a mild and non-denaturing environment for the antioxidant protein. • Maximal concentration of DPPH was li...

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Detalles Bibliográficos
Autores principales: Nicklisch, Sascha C.T., Waite, J. Herbert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4268772/
https://www.ncbi.nlm.nih.gov/pubmed/25530949
http://dx.doi.org/10.1016/j.mex.2014.10.004
Descripción
Sumario:The free radical method using 1,1-diphenyl-2-picryl-hydrazyl (DPPH) is a well established assay for the in vitro • Non-ionic detergents were added to keep the DPPH radical soluble and to provide a mild and non-denaturing environment for the antioxidant protein. • Maximal concentration of DPPH was limited to 100 μM to stay within the sensitivity range of the detector at the given wavelength (515 nm) and to increase the dynamic range of the assay. • 0.1 M citrate phosphate buffer was introduced to prevent experimental artifacts due to changing buffer compositions at different pHs.

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