Optimized DPPH assay in a detergent-based buffer system for measuring antioxidant activity of proteins

The free radical method using 1,1-diphenyl-2-picryl-hydrazyl (DPPH) is a well established assay for the in vitro • Non-ionic detergents were added to keep the DPPH radical soluble and to provide a mild and non-denaturing environment for the antioxidant protein. • Maximal concentration of DPPH was li...

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Detalles Bibliográficos
Autores principales: Nicklisch, Sascha C.T., Waite, J. Herbert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4268772/
https://www.ncbi.nlm.nih.gov/pubmed/25530949
http://dx.doi.org/10.1016/j.mex.2014.10.004
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author Nicklisch, Sascha C.T.
Waite, J. Herbert
author_facet Nicklisch, Sascha C.T.
Waite, J. Herbert
author_sort Nicklisch, Sascha C.T.
collection PubMed
description The free radical method using 1,1-diphenyl-2-picryl-hydrazyl (DPPH) is a well established assay for the in vitro • Non-ionic detergents were added to keep the DPPH radical soluble and to provide a mild and non-denaturing environment for the antioxidant protein. • Maximal concentration of DPPH was limited to 100 μM to stay within the sensitivity range of the detector at the given wavelength (515 nm) and to increase the dynamic range of the assay. • 0.1 M citrate phosphate buffer was introduced to prevent experimental artifacts due to changing buffer compositions at different pHs.
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spelling pubmed-42687722015-01-01 Optimized DPPH assay in a detergent-based buffer system for measuring antioxidant activity of proteins Nicklisch, Sascha C.T. Waite, J. Herbert MethodsX Article The free radical method using 1,1-diphenyl-2-picryl-hydrazyl (DPPH) is a well established assay for the in vitro • Non-ionic detergents were added to keep the DPPH radical soluble and to provide a mild and non-denaturing environment for the antioxidant protein. • Maximal concentration of DPPH was limited to 100 μM to stay within the sensitivity range of the detector at the given wavelength (515 nm) and to increase the dynamic range of the assay. • 0.1 M citrate phosphate buffer was introduced to prevent experimental artifacts due to changing buffer compositions at different pHs. Elsevier 2014-10-22 /pmc/articles/PMC4268772/ /pubmed/25530949 http://dx.doi.org/10.1016/j.mex.2014.10.004 Text en © 2014 The Authors http://creativecommons.org/licenses/by/3.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Nicklisch, Sascha C.T.
Waite, J. Herbert
Optimized DPPH assay in a detergent-based buffer system for measuring antioxidant activity of proteins
title Optimized DPPH assay in a detergent-based buffer system for measuring antioxidant activity of proteins
title_full Optimized DPPH assay in a detergent-based buffer system for measuring antioxidant activity of proteins
title_fullStr Optimized DPPH assay in a detergent-based buffer system for measuring antioxidant activity of proteins
title_full_unstemmed Optimized DPPH assay in a detergent-based buffer system for measuring antioxidant activity of proteins
title_short Optimized DPPH assay in a detergent-based buffer system for measuring antioxidant activity of proteins
title_sort optimized dpph assay in a detergent-based buffer system for measuring antioxidant activity of proteins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4268772/
https://www.ncbi.nlm.nih.gov/pubmed/25530949
http://dx.doi.org/10.1016/j.mex.2014.10.004
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