Cargando…
Optimized DPPH assay in a detergent-based buffer system for measuring antioxidant activity of proteins
The free radical method using 1,1-diphenyl-2-picryl-hydrazyl (DPPH) is a well established assay for the in vitro • Non-ionic detergents were added to keep the DPPH radical soluble and to provide a mild and non-denaturing environment for the antioxidant protein. • Maximal concentration of DPPH was li...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4268772/ https://www.ncbi.nlm.nih.gov/pubmed/25530949 http://dx.doi.org/10.1016/j.mex.2014.10.004 |