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Optimized DPPH assay in a detergent-based buffer system for measuring antioxidant activity of proteins

The free radical method using 1,1-diphenyl-2-picryl-hydrazyl (DPPH) is a well established assay for the in vitro • Non-ionic detergents were added to keep the DPPH radical soluble and to provide a mild and non-denaturing environment for the antioxidant protein. • Maximal concentration of DPPH was li...

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Detalles Bibliográficos
Autores principales:	Nicklisch, Sascha C.T., Waite, J. Herbert
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2014
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4268772/ https://www.ncbi.nlm.nih.gov/pubmed/25530949 http://dx.doi.org/10.1016/j.mex.2014.10.004

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