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Transient Downregulation of Nanog and Oct4 Induced by DETA/NO Exposure in Mouse Embryonic Stem Cells Leads to Mesodermal/Endodermal Lineage Differentiation

The function of pluripotency genes in differentiation is a matter of investigation. We report here that Nanog and Oct4 are reexpressed in two mouse embryonic stem cell (mESC) lines following exposure to the differentiating agent DETA/NO. Both cell lines express a battery of both endoderm and mesoder...

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Autores principales: Mora-Castilla, Sergio, Tejedo, Juan R., Tapia-Limonchi, Rafael, Díaz, Irene, Hitos, Ana B., Cahuana, Gladys M., Hmadcha, Abdelkrim, Martín, Franz, Soria, Bernat, Bedoya, Francisco J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4269087/
https://www.ncbi.nlm.nih.gov/pubmed/25544848
http://dx.doi.org/10.1155/2014/379678
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author Mora-Castilla, Sergio
Tejedo, Juan R.
Tapia-Limonchi, Rafael
Díaz, Irene
Hitos, Ana B.
Cahuana, Gladys M.
Hmadcha, Abdelkrim
Martín, Franz
Soria, Bernat
Bedoya, Francisco J.
author_facet Mora-Castilla, Sergio
Tejedo, Juan R.
Tapia-Limonchi, Rafael
Díaz, Irene
Hitos, Ana B.
Cahuana, Gladys M.
Hmadcha, Abdelkrim
Martín, Franz
Soria, Bernat
Bedoya, Francisco J.
author_sort Mora-Castilla, Sergio
collection PubMed
description The function of pluripotency genes in differentiation is a matter of investigation. We report here that Nanog and Oct4 are reexpressed in two mouse embryonic stem cell (mESC) lines following exposure to the differentiating agent DETA/NO. Both cell lines express a battery of both endoderm and mesoderm markers following induction of differentiation with DETA/NO-based protocols. Confocal analysis of cells undergoing directed differentiation shows that the majority of cells expressing Nanog express also endoderm genes such as Gata4 and FoxA2 (75.4% and 96.2%, resp.). Simultaneously, mRNA of mesodermal markers Flk1 and Mef2c are also regulated by the treatment. Acetylated histone H3 occupancy at the promoter of Nanog is involved in the process of reexpression. Furthermore, Nanog binding to the promoter of Brachyury leads to repression of this gene, thus disrupting mesendoderm transition.
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spelling pubmed-42690872014-12-28 Transient Downregulation of Nanog and Oct4 Induced by DETA/NO Exposure in Mouse Embryonic Stem Cells Leads to Mesodermal/Endodermal Lineage Differentiation Mora-Castilla, Sergio Tejedo, Juan R. Tapia-Limonchi, Rafael Díaz, Irene Hitos, Ana B. Cahuana, Gladys M. Hmadcha, Abdelkrim Martín, Franz Soria, Bernat Bedoya, Francisco J. Stem Cells Int Research Article The function of pluripotency genes in differentiation is a matter of investigation. We report here that Nanog and Oct4 are reexpressed in two mouse embryonic stem cell (mESC) lines following exposure to the differentiating agent DETA/NO. Both cell lines express a battery of both endoderm and mesoderm markers following induction of differentiation with DETA/NO-based protocols. Confocal analysis of cells undergoing directed differentiation shows that the majority of cells expressing Nanog express also endoderm genes such as Gata4 and FoxA2 (75.4% and 96.2%, resp.). Simultaneously, mRNA of mesodermal markers Flk1 and Mef2c are also regulated by the treatment. Acetylated histone H3 occupancy at the promoter of Nanog is involved in the process of reexpression. Furthermore, Nanog binding to the promoter of Brachyury leads to repression of this gene, thus disrupting mesendoderm transition. Hindawi Publishing Corporation 2014 2014-12-03 /pmc/articles/PMC4269087/ /pubmed/25544848 http://dx.doi.org/10.1155/2014/379678 Text en Copyright © 2014 Sergio Mora-Castilla et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Mora-Castilla, Sergio
Tejedo, Juan R.
Tapia-Limonchi, Rafael
Díaz, Irene
Hitos, Ana B.
Cahuana, Gladys M.
Hmadcha, Abdelkrim
Martín, Franz
Soria, Bernat
Bedoya, Francisco J.
Transient Downregulation of Nanog and Oct4 Induced by DETA/NO Exposure in Mouse Embryonic Stem Cells Leads to Mesodermal/Endodermal Lineage Differentiation
title Transient Downregulation of Nanog and Oct4 Induced by DETA/NO Exposure in Mouse Embryonic Stem Cells Leads to Mesodermal/Endodermal Lineage Differentiation
title_full Transient Downregulation of Nanog and Oct4 Induced by DETA/NO Exposure in Mouse Embryonic Stem Cells Leads to Mesodermal/Endodermal Lineage Differentiation
title_fullStr Transient Downregulation of Nanog and Oct4 Induced by DETA/NO Exposure in Mouse Embryonic Stem Cells Leads to Mesodermal/Endodermal Lineage Differentiation
title_full_unstemmed Transient Downregulation of Nanog and Oct4 Induced by DETA/NO Exposure in Mouse Embryonic Stem Cells Leads to Mesodermal/Endodermal Lineage Differentiation
title_short Transient Downregulation of Nanog and Oct4 Induced by DETA/NO Exposure in Mouse Embryonic Stem Cells Leads to Mesodermal/Endodermal Lineage Differentiation
title_sort transient downregulation of nanog and oct4 induced by deta/no exposure in mouse embryonic stem cells leads to mesodermal/endodermal lineage differentiation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4269087/
https://www.ncbi.nlm.nih.gov/pubmed/25544848
http://dx.doi.org/10.1155/2014/379678
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