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Transient Downregulation of Nanog and Oct4 Induced by DETA/NO Exposure in Mouse Embryonic Stem Cells Leads to Mesodermal/Endodermal Lineage Differentiation
The function of pluripotency genes in differentiation is a matter of investigation. We report here that Nanog and Oct4 are reexpressed in two mouse embryonic stem cell (mESC) lines following exposure to the differentiating agent DETA/NO. Both cell lines express a battery of both endoderm and mesoder...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4269087/ https://www.ncbi.nlm.nih.gov/pubmed/25544848 http://dx.doi.org/10.1155/2014/379678 |
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author | Mora-Castilla, Sergio Tejedo, Juan R. Tapia-Limonchi, Rafael Díaz, Irene Hitos, Ana B. Cahuana, Gladys M. Hmadcha, Abdelkrim Martín, Franz Soria, Bernat Bedoya, Francisco J. |
author_facet | Mora-Castilla, Sergio Tejedo, Juan R. Tapia-Limonchi, Rafael Díaz, Irene Hitos, Ana B. Cahuana, Gladys M. Hmadcha, Abdelkrim Martín, Franz Soria, Bernat Bedoya, Francisco J. |
author_sort | Mora-Castilla, Sergio |
collection | PubMed |
description | The function of pluripotency genes in differentiation is a matter of investigation. We report here that Nanog and Oct4 are reexpressed in two mouse embryonic stem cell (mESC) lines following exposure to the differentiating agent DETA/NO. Both cell lines express a battery of both endoderm and mesoderm markers following induction of differentiation with DETA/NO-based protocols. Confocal analysis of cells undergoing directed differentiation shows that the majority of cells expressing Nanog express also endoderm genes such as Gata4 and FoxA2 (75.4% and 96.2%, resp.). Simultaneously, mRNA of mesodermal markers Flk1 and Mef2c are also regulated by the treatment. Acetylated histone H3 occupancy at the promoter of Nanog is involved in the process of reexpression. Furthermore, Nanog binding to the promoter of Brachyury leads to repression of this gene, thus disrupting mesendoderm transition. |
format | Online Article Text |
id | pubmed-4269087 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-42690872014-12-28 Transient Downregulation of Nanog and Oct4 Induced by DETA/NO Exposure in Mouse Embryonic Stem Cells Leads to Mesodermal/Endodermal Lineage Differentiation Mora-Castilla, Sergio Tejedo, Juan R. Tapia-Limonchi, Rafael Díaz, Irene Hitos, Ana B. Cahuana, Gladys M. Hmadcha, Abdelkrim Martín, Franz Soria, Bernat Bedoya, Francisco J. Stem Cells Int Research Article The function of pluripotency genes in differentiation is a matter of investigation. We report here that Nanog and Oct4 are reexpressed in two mouse embryonic stem cell (mESC) lines following exposure to the differentiating agent DETA/NO. Both cell lines express a battery of both endoderm and mesoderm markers following induction of differentiation with DETA/NO-based protocols. Confocal analysis of cells undergoing directed differentiation shows that the majority of cells expressing Nanog express also endoderm genes such as Gata4 and FoxA2 (75.4% and 96.2%, resp.). Simultaneously, mRNA of mesodermal markers Flk1 and Mef2c are also regulated by the treatment. Acetylated histone H3 occupancy at the promoter of Nanog is involved in the process of reexpression. Furthermore, Nanog binding to the promoter of Brachyury leads to repression of this gene, thus disrupting mesendoderm transition. Hindawi Publishing Corporation 2014 2014-12-03 /pmc/articles/PMC4269087/ /pubmed/25544848 http://dx.doi.org/10.1155/2014/379678 Text en Copyright © 2014 Sergio Mora-Castilla et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Mora-Castilla, Sergio Tejedo, Juan R. Tapia-Limonchi, Rafael Díaz, Irene Hitos, Ana B. Cahuana, Gladys M. Hmadcha, Abdelkrim Martín, Franz Soria, Bernat Bedoya, Francisco J. Transient Downregulation of Nanog and Oct4 Induced by DETA/NO Exposure in Mouse Embryonic Stem Cells Leads to Mesodermal/Endodermal Lineage Differentiation |
title | Transient Downregulation of Nanog and Oct4 Induced by DETA/NO Exposure in Mouse Embryonic Stem Cells Leads to Mesodermal/Endodermal Lineage Differentiation |
title_full | Transient Downregulation of Nanog and Oct4 Induced by DETA/NO Exposure in Mouse Embryonic Stem Cells Leads to Mesodermal/Endodermal Lineage Differentiation |
title_fullStr | Transient Downregulation of Nanog and Oct4 Induced by DETA/NO Exposure in Mouse Embryonic Stem Cells Leads to Mesodermal/Endodermal Lineage Differentiation |
title_full_unstemmed | Transient Downregulation of Nanog and Oct4 Induced by DETA/NO Exposure in Mouse Embryonic Stem Cells Leads to Mesodermal/Endodermal Lineage Differentiation |
title_short | Transient Downregulation of Nanog and Oct4 Induced by DETA/NO Exposure in Mouse Embryonic Stem Cells Leads to Mesodermal/Endodermal Lineage Differentiation |
title_sort | transient downregulation of nanog and oct4 induced by deta/no exposure in mouse embryonic stem cells leads to mesodermal/endodermal lineage differentiation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4269087/ https://www.ncbi.nlm.nih.gov/pubmed/25544848 http://dx.doi.org/10.1155/2014/379678 |
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