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Significant expression of a Chinese scorpion peptide, BmK1, in Escherichia coli through promoter engineering and gene dosage strategy

Heterologous expression is an efficient alternative to conventional extraction to produce a specific Buthus martensii Karsch (BmK) peptide. In this work, BmK1 was successfully expressed in Escherichia coli after genetic codon optimization, but BmK1 content was <6% of total cellular protein. To im...

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Detalles Bibliográficos
Autores principales:	Wang, Jianfeng, Xiong, Zhiqiang, Yang, Yingying, Zhao, Na, Wang, Yong
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BlackWell Publishing Ltd 2014
Materias:	Original Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4269186/ https://www.ncbi.nlm.nih.gov/pubmed/24372571 http://dx.doi.org/10.1002/bab.1194

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