Specificity Study on Concentration of Monoamine Transmitters at Acupoint and Effect of Acupuncture on Its Distribution

To investigate the distribution of monoamine transmitters at acupoints and effect of acupuncture on it. Take this experiment by means of microdialysis. Twenty rabbits were randomly assigned to two groups (Group A: acupuncture Guanyuan (RN4), Group B: acupuncture nonacupoint which is besides Guanyuan (RN4) 1 cm). Before and after acupuncture was taken, tissue fluids both at Zhongwan (RN12) which is on the same meridian as Guanyuan and at a nonacupoint 1 cm away from Zhongwan were collected through microdialysis, respectively. The collected samples were analyzed to determine concentrations of monoamine transmitters. Epinephrine and 5-HT were detected. An unknown substance was found. Its concentration at acupoint was significantly higher than that at nonacupoint and decreased after acupuncture. Its significant specificity at acupoints suggests that it may play an important role in meridian's activity.