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A host beetle pheromone regulates development and behavior in the nematode Pristionchus pacificus

Nematodes and insects are the two most speciose animal phyla and nematode–insect associations encompass widespread biological interactions. To dissect the chemical signals and the genes mediating this association, we investigated the effect of an oriental beetle sex pheromone on the development and...

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Autores principales: Cinkornpumin, Jessica K, Wisidagama, Dona R, Rapoport, Veronika, Go, James L, Dieterich, Christoph, Wang, Xiaoyue, Sommer, Ralf J, Hong, Ray L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4270288/
https://www.ncbi.nlm.nih.gov/pubmed/25317948
http://dx.doi.org/10.7554/eLife.03229
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author Cinkornpumin, Jessica K
Wisidagama, Dona R
Rapoport, Veronika
Go, James L
Dieterich, Christoph
Wang, Xiaoyue
Sommer, Ralf J
Hong, Ray L
author_facet Cinkornpumin, Jessica K
Wisidagama, Dona R
Rapoport, Veronika
Go, James L
Dieterich, Christoph
Wang, Xiaoyue
Sommer, Ralf J
Hong, Ray L
author_sort Cinkornpumin, Jessica K
collection PubMed
description Nematodes and insects are the two most speciose animal phyla and nematode–insect associations encompass widespread biological interactions. To dissect the chemical signals and the genes mediating this association, we investigated the effect of an oriental beetle sex pheromone on the development and behavior of the nematode Pristionchus pacificus. We found that while the beetle pheromone is attractive to P. pacificus adults, the pheromone arrests embryo development, paralyzes J2 larva, and inhibits exit of dauer larvae. To uncover the mechanism that regulates insect pheromone sensitivity, a newly identified mutant, Ppa-obi-1, is used to reveal the molecular links between altered attraction towards the beetle pheromone, as well as hypersensitivity to its paralyzing effects. Ppa-obi-1 encodes lipid-binding domains and reaches its highest expression in various cell types, including the amphid neuron sheath and excretory cells. Our data suggest that the beetle host pheromone may be a species-specific volatile synomone that co-evolved with necromeny. DOI: http://dx.doi.org/10.7554/eLife.03229.001
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spelling pubmed-42702882015-01-29 A host beetle pheromone regulates development and behavior in the nematode Pristionchus pacificus Cinkornpumin, Jessica K Wisidagama, Dona R Rapoport, Veronika Go, James L Dieterich, Christoph Wang, Xiaoyue Sommer, Ralf J Hong, Ray L eLife Developmental Biology and Stem Cells Nematodes and insects are the two most speciose animal phyla and nematode–insect associations encompass widespread biological interactions. To dissect the chemical signals and the genes mediating this association, we investigated the effect of an oriental beetle sex pheromone on the development and behavior of the nematode Pristionchus pacificus. We found that while the beetle pheromone is attractive to P. pacificus adults, the pheromone arrests embryo development, paralyzes J2 larva, and inhibits exit of dauer larvae. To uncover the mechanism that regulates insect pheromone sensitivity, a newly identified mutant, Ppa-obi-1, is used to reveal the molecular links between altered attraction towards the beetle pheromone, as well as hypersensitivity to its paralyzing effects. Ppa-obi-1 encodes lipid-binding domains and reaches its highest expression in various cell types, including the amphid neuron sheath and excretory cells. Our data suggest that the beetle host pheromone may be a species-specific volatile synomone that co-evolved with necromeny. DOI: http://dx.doi.org/10.7554/eLife.03229.001 eLife Sciences Publications, Ltd 2014-10-15 /pmc/articles/PMC4270288/ /pubmed/25317948 http://dx.doi.org/10.7554/eLife.03229 Text en © 2014, Cinkornpumin et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Developmental Biology and Stem Cells
Cinkornpumin, Jessica K
Wisidagama, Dona R
Rapoport, Veronika
Go, James L
Dieterich, Christoph
Wang, Xiaoyue
Sommer, Ralf J
Hong, Ray L
A host beetle pheromone regulates development and behavior in the nematode Pristionchus pacificus
title A host beetle pheromone regulates development and behavior in the nematode Pristionchus pacificus
title_full A host beetle pheromone regulates development and behavior in the nematode Pristionchus pacificus
title_fullStr A host beetle pheromone regulates development and behavior in the nematode Pristionchus pacificus
title_full_unstemmed A host beetle pheromone regulates development and behavior in the nematode Pristionchus pacificus
title_short A host beetle pheromone regulates development and behavior in the nematode Pristionchus pacificus
title_sort host beetle pheromone regulates development and behavior in the nematode pristionchus pacificus
topic Developmental Biology and Stem Cells
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4270288/
https://www.ncbi.nlm.nih.gov/pubmed/25317948
http://dx.doi.org/10.7554/eLife.03229
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