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Paxillin regulates vascular endothelial growth factor A-induced in vitro angiogenesis of human umbilical vein endothelial cells

The purpose of the present study was to investigate the role of paxillin in the vascular endothelial growth factor A (VEGF-A)-induced adhesion, proliferation, migration and capillary formation of endothelial cells (ECs) in vitro. Human umbilical vein ECs (HUVECs) were used to evaluate these four pro...

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Autores principales: YANG, WAN-JU, YANG, YAN-NING, CAO, JIN, MAN, ZI-HUI, LI, YING, XING, YI-QIAO
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4270338/
https://www.ncbi.nlm.nih.gov/pubmed/25405379
http://dx.doi.org/10.3892/mmr.2014.2961
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author YANG, WAN-JU
YANG, YAN-NING
CAO, JIN
MAN, ZI-HUI
LI, YING
XING, YI-QIAO
author_facet YANG, WAN-JU
YANG, YAN-NING
CAO, JIN
MAN, ZI-HUI
LI, YING
XING, YI-QIAO
author_sort YANG, WAN-JU
collection PubMed
description The purpose of the present study was to investigate the role of paxillin in the vascular endothelial growth factor A (VEGF-A)-induced adhesion, proliferation, migration and capillary formation of endothelial cells (ECs) in vitro. Human umbilical vein ECs (HUVECs) were used to evaluate these four processes in vitro. The HUVECs were either mock-transfected (control), transfected with scramble small interference RNA (siRNA) or transfected with siRNA specifically targeting paxillin. VEGF-A (20 ng/ml) was used to stimulate angiogenesis. The VEGF-A treatment significantly increased the adhesion, proliferation, migration and tube formation of the HUVECs in the control and scramble siRNA groups, whereas the siRNA-mediated knockdown of paxillin inhibited these VEGF-A-induced effects. Paxillin is essential for VEGF-A-mediated angiogenesis in ECs and its inhibition may be a potential target for antiangiogenic therapies.
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spelling pubmed-42703382014-12-19 Paxillin regulates vascular endothelial growth factor A-induced in vitro angiogenesis of human umbilical vein endothelial cells YANG, WAN-JU YANG, YAN-NING CAO, JIN MAN, ZI-HUI LI, YING XING, YI-QIAO Mol Med Rep Articles The purpose of the present study was to investigate the role of paxillin in the vascular endothelial growth factor A (VEGF-A)-induced adhesion, proliferation, migration and capillary formation of endothelial cells (ECs) in vitro. Human umbilical vein ECs (HUVECs) were used to evaluate these four processes in vitro. The HUVECs were either mock-transfected (control), transfected with scramble small interference RNA (siRNA) or transfected with siRNA specifically targeting paxillin. VEGF-A (20 ng/ml) was used to stimulate angiogenesis. The VEGF-A treatment significantly increased the adhesion, proliferation, migration and tube formation of the HUVECs in the control and scramble siRNA groups, whereas the siRNA-mediated knockdown of paxillin inhibited these VEGF-A-induced effects. Paxillin is essential for VEGF-A-mediated angiogenesis in ECs and its inhibition may be a potential target for antiangiogenic therapies. D.A. Spandidos 2015-03 2014-11-17 /pmc/articles/PMC4270338/ /pubmed/25405379 http://dx.doi.org/10.3892/mmr.2014.2961 Text en Copyright © 2015, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
YANG, WAN-JU
YANG, YAN-NING
CAO, JIN
MAN, ZI-HUI
LI, YING
XING, YI-QIAO
Paxillin regulates vascular endothelial growth factor A-induced in vitro angiogenesis of human umbilical vein endothelial cells
title Paxillin regulates vascular endothelial growth factor A-induced in vitro angiogenesis of human umbilical vein endothelial cells
title_full Paxillin regulates vascular endothelial growth factor A-induced in vitro angiogenesis of human umbilical vein endothelial cells
title_fullStr Paxillin regulates vascular endothelial growth factor A-induced in vitro angiogenesis of human umbilical vein endothelial cells
title_full_unstemmed Paxillin regulates vascular endothelial growth factor A-induced in vitro angiogenesis of human umbilical vein endothelial cells
title_short Paxillin regulates vascular endothelial growth factor A-induced in vitro angiogenesis of human umbilical vein endothelial cells
title_sort paxillin regulates vascular endothelial growth factor a-induced in vitro angiogenesis of human umbilical vein endothelial cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4270338/
https://www.ncbi.nlm.nih.gov/pubmed/25405379
http://dx.doi.org/10.3892/mmr.2014.2961
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