A Recombinant Avian Leukosis Virus Subgroup J for Directly Monitoring Viral Infection and the Selection of Neutralizing Antibodies

Avian leukosis virus subgroup J (ALV-J) has induced serious clinical outbreaks and has become a serious infectious disease of chickens in China. We describe here the creation of a recombinant ALV-J tagged with the enhanced green fluorescent protein (named rHPRS-103EGFP). We successfully utilize the...

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Detalles Bibliográficos
Autores principales: Wang, Qi, Li, Xiaofei, Ji, Xiaolin, Wang, Jingfei, Shen, Nan, Gao, Yulong, Qi, Xiaole, Wang, Yongqiang, Gao, Honglei, Zhang, Shide, Wang, Xiaomei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4270768/
https://www.ncbi.nlm.nih.gov/pubmed/25522008
http://dx.doi.org/10.1371/journal.pone.0115422
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author Wang, Qi
Li, Xiaofei
Ji, Xiaolin
Wang, Jingfei
Shen, Nan
Gao, Yulong
Qi, Xiaole
Wang, Yongqiang
Gao, Honglei
Zhang, Shide
Wang, Xiaomei
author_facet Wang, Qi
Li, Xiaofei
Ji, Xiaolin
Wang, Jingfei
Shen, Nan
Gao, Yulong
Qi, Xiaole
Wang, Yongqiang
Gao, Honglei
Zhang, Shide
Wang, Xiaomei
author_sort Wang, Qi
collection PubMed
description Avian leukosis virus subgroup J (ALV-J) has induced serious clinical outbreaks and has become a serious infectious disease of chickens in China. We describe here the creation of a recombinant ALV-J tagged with the enhanced green fluorescent protein (named rHPRS-103EGFP). We successfully utilize the rHPRS-103EGFP to visualize viral infection and for development of a simplified serum-neutralization test.
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spelling pubmed-42707682014-12-26 A Recombinant Avian Leukosis Virus Subgroup J for Directly Monitoring Viral Infection and the Selection of Neutralizing Antibodies Wang, Qi Li, Xiaofei Ji, Xiaolin Wang, Jingfei Shen, Nan Gao, Yulong Qi, Xiaole Wang, Yongqiang Gao, Honglei Zhang, Shide Wang, Xiaomei PLoS One Research Article Avian leukosis virus subgroup J (ALV-J) has induced serious clinical outbreaks and has become a serious infectious disease of chickens in China. We describe here the creation of a recombinant ALV-J tagged with the enhanced green fluorescent protein (named rHPRS-103EGFP). We successfully utilize the rHPRS-103EGFP to visualize viral infection and for development of a simplified serum-neutralization test. Public Library of Science 2014-12-18 /pmc/articles/PMC4270768/ /pubmed/25522008 http://dx.doi.org/10.1371/journal.pone.0115422 Text en © 2014 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wang, Qi
Li, Xiaofei
Ji, Xiaolin
Wang, Jingfei
Shen, Nan
Gao, Yulong
Qi, Xiaole
Wang, Yongqiang
Gao, Honglei
Zhang, Shide
Wang, Xiaomei
A Recombinant Avian Leukosis Virus Subgroup J for Directly Monitoring Viral Infection and the Selection of Neutralizing Antibodies
title A Recombinant Avian Leukosis Virus Subgroup J for Directly Monitoring Viral Infection and the Selection of Neutralizing Antibodies
title_full A Recombinant Avian Leukosis Virus Subgroup J for Directly Monitoring Viral Infection and the Selection of Neutralizing Antibodies
title_fullStr A Recombinant Avian Leukosis Virus Subgroup J for Directly Monitoring Viral Infection and the Selection of Neutralizing Antibodies
title_full_unstemmed A Recombinant Avian Leukosis Virus Subgroup J for Directly Monitoring Viral Infection and the Selection of Neutralizing Antibodies
title_short A Recombinant Avian Leukosis Virus Subgroup J for Directly Monitoring Viral Infection and the Selection of Neutralizing Antibodies
title_sort recombinant avian leukosis virus subgroup j for directly monitoring viral infection and the selection of neutralizing antibodies
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4270768/
https://www.ncbi.nlm.nih.gov/pubmed/25522008
http://dx.doi.org/10.1371/journal.pone.0115422
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