Cargando…
Downregulation of microRNA-100 protects apoptosis and promotes neuronal growth in retinal ganglion cells
BACKGROUND: Retinal ganglion cells (RGCs) are preferentially lost in glaucoma or optic neuritis. In the present study, we investigated the protective effect of mircoRNA 100 (miR-100) against oxidative stress induced apoptosis in RGC-5 cells. RESULTS: Rat RGC-5 cells were cultured in plates and H(2)O...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4271342/ https://www.ncbi.nlm.nih.gov/pubmed/25406880 http://dx.doi.org/10.1186/s12867-014-0025-1 |
_version_ | 1782349586461884416 |
---|---|
author | Kong, Ning Lu, Xiaohe Li, Bin |
author_facet | Kong, Ning Lu, Xiaohe Li, Bin |
author_sort | Kong, Ning |
collection | PubMed |
description | BACKGROUND: Retinal ganglion cells (RGCs) are preferentially lost in glaucoma or optic neuritis. In the present study, we investigated the protective effect of mircoRNA 100 (miR-100) against oxidative stress induced apoptosis in RGC-5 cells. RESULTS: Rat RGC-5 cells were cultured in plates and H(2)O(2) was added to induce oxidative stress. TUNEL assay and qRT-PCR showed H(2)O(2) induced apoptosis and up-regulated miR-100 in a dose-dependent manner in RGC-5 cells. Conversely, lentiviral-mediated miR-100 down-regulation protected H(2)O(2) induced apoptosis, promoted neurite growth and activated AKT/ERK and TrkB pathways through phosphorylation. Luciferase assay confirmed that IGF1R was directly regulated by miR-100 in RGC-5 cells, and siRNA-mediated IGF1R knockdown activated AKT protein through phosphorylation, down-regulated miR-100, therefore exerted a protective effect on RGC-5 apoptosis. CONCLUSION: Down-regulating miR-100 is an effective method to protect H(2)O(2) induced apoptosis in RGC-5 cells, possible associated with IGF1R regulation. |
format | Online Article Text |
id | pubmed-4271342 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-42713422014-12-20 Downregulation of microRNA-100 protects apoptosis and promotes neuronal growth in retinal ganglion cells Kong, Ning Lu, Xiaohe Li, Bin BMC Mol Biol Research Article BACKGROUND: Retinal ganglion cells (RGCs) are preferentially lost in glaucoma or optic neuritis. In the present study, we investigated the protective effect of mircoRNA 100 (miR-100) against oxidative stress induced apoptosis in RGC-5 cells. RESULTS: Rat RGC-5 cells were cultured in plates and H(2)O(2) was added to induce oxidative stress. TUNEL assay and qRT-PCR showed H(2)O(2) induced apoptosis and up-regulated miR-100 in a dose-dependent manner in RGC-5 cells. Conversely, lentiviral-mediated miR-100 down-regulation protected H(2)O(2) induced apoptosis, promoted neurite growth and activated AKT/ERK and TrkB pathways through phosphorylation. Luciferase assay confirmed that IGF1R was directly regulated by miR-100 in RGC-5 cells, and siRNA-mediated IGF1R knockdown activated AKT protein through phosphorylation, down-regulated miR-100, therefore exerted a protective effect on RGC-5 apoptosis. CONCLUSION: Down-regulating miR-100 is an effective method to protect H(2)O(2) induced apoptosis in RGC-5 cells, possible associated with IGF1R regulation. BioMed Central 2014-11-18 /pmc/articles/PMC4271342/ /pubmed/25406880 http://dx.doi.org/10.1186/s12867-014-0025-1 Text en © Kong et al.; licensee BioMed Central Ltd. 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Kong, Ning Lu, Xiaohe Li, Bin Downregulation of microRNA-100 protects apoptosis and promotes neuronal growth in retinal ganglion cells |
title | Downregulation of microRNA-100 protects apoptosis and promotes neuronal growth in retinal ganglion cells |
title_full | Downregulation of microRNA-100 protects apoptosis and promotes neuronal growth in retinal ganglion cells |
title_fullStr | Downregulation of microRNA-100 protects apoptosis and promotes neuronal growth in retinal ganglion cells |
title_full_unstemmed | Downregulation of microRNA-100 protects apoptosis and promotes neuronal growth in retinal ganglion cells |
title_short | Downregulation of microRNA-100 protects apoptosis and promotes neuronal growth in retinal ganglion cells |
title_sort | downregulation of microrna-100 protects apoptosis and promotes neuronal growth in retinal ganglion cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4271342/ https://www.ncbi.nlm.nih.gov/pubmed/25406880 http://dx.doi.org/10.1186/s12867-014-0025-1 |
work_keys_str_mv | AT kongning downregulationofmicrorna100protectsapoptosisandpromotesneuronalgrowthinretinalganglioncells AT luxiaohe downregulationofmicrorna100protectsapoptosisandpromotesneuronalgrowthinretinalganglioncells AT libin downregulationofmicrorna100protectsapoptosisandpromotesneuronalgrowthinretinalganglioncells |