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Anthelmintic effect of carob pods and sainfoin hay when fed to lambs after experimental trickle infections with Haemonchus contortus and Trichostrongylus colubriformis

The aim of the study was to compare the in vivo anthelmintic activity of sainfoin hay (Onobrychis viciifolia) and carob pod meal (Ceratonia siliqua) against gastrointestinal nematodes. Seven days before infection, 64 naive lambs were assigned to four different groups: Group S received sainfoin hay a...

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Autores principales: Arroyo-Lopez, Celia, Manolaraki, Foteini, Saratsis, Anastasios, Saratsi, Katerina, Stefanakis, Alexandros, Skampardonis, Vasileios, Voutzourakis, Nikolaos, Hoste, Hervé, Sotiraki, Smaragda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4271655/
https://www.ncbi.nlm.nih.gov/pubmed/25526546
http://dx.doi.org/10.1051/parasite/2014074
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author Arroyo-Lopez, Celia
Manolaraki, Foteini
Saratsis, Anastasios
Saratsi, Katerina
Stefanakis, Alexandros
Skampardonis, Vasileios
Voutzourakis, Nikolaos
Hoste, Hervé
Sotiraki, Smaragda
author_facet Arroyo-Lopez, Celia
Manolaraki, Foteini
Saratsis, Anastasios
Saratsi, Katerina
Stefanakis, Alexandros
Skampardonis, Vasileios
Voutzourakis, Nikolaos
Hoste, Hervé
Sotiraki, Smaragda
author_sort Arroyo-Lopez, Celia
collection PubMed
description The aim of the study was to compare the in vivo anthelmintic activity of sainfoin hay (Onobrychis viciifolia) and carob pod meal (Ceratonia siliqua) against gastrointestinal nematodes. Seven days before infection, 64 naive lambs were assigned to four different groups: Group S received sainfoin hay and group CAR was fed with carob pods. The remaining lambs received lucerne hay (Medicago sativa) and were assigned to positive (non-treated, NT) and negative (treated, T) control groups (treatment with albendazole). On day 0, lambs were artificially trickle infected for 6 weeks, with a mixture of infective larvae of Haemonchus contortus and Trichostrongylus colubriformis. Parasitological and pathophysiological parameters were measured repeatedly during the 2-month study. Compared to the NT group, decreases in egg excretion were observed in the CAR and S groups with significant differences only found for sainfoin (p < 0.05). At necropsy, group S showed decreases in the total worm numbers of both nematode species with significant differences for H. contortus. In contrast, no differences were noticed for the CAR group. Compared to the NT group, lower values for fecundity of female H. contortus were found in the S and CAR groups, however differences were non-significant. No differences in body weight gains were found between groups. Consistent results were found showing significantly higher packed cell volume (PCV) values in the T and S groups compared to NT and CAR groups. Overall, these results confirm a positive effect associated with the feeding of lambs with tanniniferous resources on host resilience (PCV values) and against gastrointestinal parasitic nematodes by affecting some biological traits of worm populations (e.g. eggs per gram of faeces and worm numbers). However, the anthelmintic effects differed between the two tannin-containing resources, which might be associated with the quantity and/or quality of secondary metabolites (condensed tannins and/or other polyphenols).
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spelling pubmed-42716552014-12-29 Anthelmintic effect of carob pods and sainfoin hay when fed to lambs after experimental trickle infections with Haemonchus contortus and Trichostrongylus colubriformis Arroyo-Lopez, Celia Manolaraki, Foteini Saratsis, Anastasios Saratsi, Katerina Stefanakis, Alexandros Skampardonis, Vasileios Voutzourakis, Nikolaos Hoste, Hervé Sotiraki, Smaragda Parasite Research Article The aim of the study was to compare the in vivo anthelmintic activity of sainfoin hay (Onobrychis viciifolia) and carob pod meal (Ceratonia siliqua) against gastrointestinal nematodes. Seven days before infection, 64 naive lambs were assigned to four different groups: Group S received sainfoin hay and group CAR was fed with carob pods. The remaining lambs received lucerne hay (Medicago sativa) and were assigned to positive (non-treated, NT) and negative (treated, T) control groups (treatment with albendazole). On day 0, lambs were artificially trickle infected for 6 weeks, with a mixture of infective larvae of Haemonchus contortus and Trichostrongylus colubriformis. Parasitological and pathophysiological parameters were measured repeatedly during the 2-month study. Compared to the NT group, decreases in egg excretion were observed in the CAR and S groups with significant differences only found for sainfoin (p < 0.05). At necropsy, group S showed decreases in the total worm numbers of both nematode species with significant differences for H. contortus. In contrast, no differences were noticed for the CAR group. Compared to the NT group, lower values for fecundity of female H. contortus were found in the S and CAR groups, however differences were non-significant. No differences in body weight gains were found between groups. Consistent results were found showing significantly higher packed cell volume (PCV) values in the T and S groups compared to NT and CAR groups. Overall, these results confirm a positive effect associated with the feeding of lambs with tanniniferous resources on host resilience (PCV values) and against gastrointestinal parasitic nematodes by affecting some biological traits of worm populations (e.g. eggs per gram of faeces and worm numbers). However, the anthelmintic effects differed between the two tannin-containing resources, which might be associated with the quantity and/or quality of secondary metabolites (condensed tannins and/or other polyphenols). EDP Sciences 2014 2014-12-22 /pmc/articles/PMC4271655/ /pubmed/25526546 http://dx.doi.org/10.1051/parasite/2014074 Text en © C. Arroyo-Lopez et al., published by EDP Sciences, 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Arroyo-Lopez, Celia
Manolaraki, Foteini
Saratsis, Anastasios
Saratsi, Katerina
Stefanakis, Alexandros
Skampardonis, Vasileios
Voutzourakis, Nikolaos
Hoste, Hervé
Sotiraki, Smaragda
Anthelmintic effect of carob pods and sainfoin hay when fed to lambs after experimental trickle infections with Haemonchus contortus and Trichostrongylus colubriformis
title Anthelmintic effect of carob pods and sainfoin hay when fed to lambs after experimental trickle infections with Haemonchus contortus and Trichostrongylus colubriformis
title_full Anthelmintic effect of carob pods and sainfoin hay when fed to lambs after experimental trickle infections with Haemonchus contortus and Trichostrongylus colubriformis
title_fullStr Anthelmintic effect of carob pods and sainfoin hay when fed to lambs after experimental trickle infections with Haemonchus contortus and Trichostrongylus colubriformis
title_full_unstemmed Anthelmintic effect of carob pods and sainfoin hay when fed to lambs after experimental trickle infections with Haemonchus contortus and Trichostrongylus colubriformis
title_short Anthelmintic effect of carob pods and sainfoin hay when fed to lambs after experimental trickle infections with Haemonchus contortus and Trichostrongylus colubriformis
title_sort anthelmintic effect of carob pods and sainfoin hay when fed to lambs after experimental trickle infections with haemonchus contortus and trichostrongylus colubriformis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4271655/
https://www.ncbi.nlm.nih.gov/pubmed/25526546
http://dx.doi.org/10.1051/parasite/2014074
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