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Protective Effects of Lithospermum erythrorhizon Against Cerulein-Induced Acute Pancreatitis

OBJECTIVES: We aimed to evaluate the anti-inflammatory and inhibitory effects of Lithospermum erythrorhizon (LE) on cerulein-induced acute pancreatitis (AP) in a mouse model. METHODS: Acute pancreatitis was induced via intraperitoneal injection of cerulein (50 μg/kg) every hour for 6 times. In the L...

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Autores principales: Choi, Sun Bok, Bae, Gi-Sang, Jo, Il-Joo, Seo, Seung-Hee, Kim, Dong-Goo, Shin, Joon-Yeon, Hong, Seung-Heon, Choi, Byung-Min, Park, Sang-Hyun, Song, Ho-Joon, Park, Sung-Joo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4272226/
https://www.ncbi.nlm.nih.gov/pubmed/25102438
http://dx.doi.org/10.1097/MPA.0000000000000195
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author Choi, Sun Bok
Bae, Gi-Sang
Jo, Il-Joo
Seo, Seung-Hee
Kim, Dong-Goo
Shin, Joon-Yeon
Hong, Seung-Heon
Choi, Byung-Min
Park, Sang-Hyun
Song, Ho-Joon
Park, Sung-Joo
author_facet Choi, Sun Bok
Bae, Gi-Sang
Jo, Il-Joo
Seo, Seung-Hee
Kim, Dong-Goo
Shin, Joon-Yeon
Hong, Seung-Heon
Choi, Byung-Min
Park, Sang-Hyun
Song, Ho-Joon
Park, Sung-Joo
author_sort Choi, Sun Bok
collection PubMed
description OBJECTIVES: We aimed to evaluate the anti-inflammatory and inhibitory effects of Lithospermum erythrorhizon (LE) on cerulein-induced acute pancreatitis (AP) in a mouse model. METHODS: Acute pancreatitis was induced via intraperitoneal injection of cerulein (50 μg/kg) every hour for 6 times. In the LE, water extract (100, 250, or 500 mg/kg) was administered intraperitoneally 1 hour before the first injection of cerulein. Six hours after AP, blood, the pancreas, and the lung were harvested for further examination. In addition, pancreatic acinar cells were isolated using a collagenase method, and then, we investigated the acinar cell viability and cytokine productions. RESULTS: Treatment with LE reduced pancreatic damage and AP-associated lung injury and attenuated the severity of AP, as evidenced by the reduction in neutrophil infiltration, serum amylase and lipase levels, trypsin activity, and proinflammatory cytokine expression. In addition, treatment with LE inhibited high mobility group box 1 expression in the pancreas during AP. In accordance with in vivo data, LE inhibited the cerulein-induced acinar cell death, cytokine productions, and high-mobility group box 1 expression. Furthermore, LE also inhibited the activation of p38 mitogen-activated protein kinases. CONCLUSIONS: These results suggest that LE plays a protective role during the development of AP by inhibiting the activation of p38.
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spelling pubmed-42722262014-12-23 Protective Effects of Lithospermum erythrorhizon Against Cerulein-Induced Acute Pancreatitis Choi, Sun Bok Bae, Gi-Sang Jo, Il-Joo Seo, Seung-Hee Kim, Dong-Goo Shin, Joon-Yeon Hong, Seung-Heon Choi, Byung-Min Park, Sang-Hyun Song, Ho-Joon Park, Sung-Joo Pancreas Original Articles OBJECTIVES: We aimed to evaluate the anti-inflammatory and inhibitory effects of Lithospermum erythrorhizon (LE) on cerulein-induced acute pancreatitis (AP) in a mouse model. METHODS: Acute pancreatitis was induced via intraperitoneal injection of cerulein (50 μg/kg) every hour for 6 times. In the LE, water extract (100, 250, or 500 mg/kg) was administered intraperitoneally 1 hour before the first injection of cerulein. Six hours after AP, blood, the pancreas, and the lung were harvested for further examination. In addition, pancreatic acinar cells were isolated using a collagenase method, and then, we investigated the acinar cell viability and cytokine productions. RESULTS: Treatment with LE reduced pancreatic damage and AP-associated lung injury and attenuated the severity of AP, as evidenced by the reduction in neutrophil infiltration, serum amylase and lipase levels, trypsin activity, and proinflammatory cytokine expression. In addition, treatment with LE inhibited high mobility group box 1 expression in the pancreas during AP. In accordance with in vivo data, LE inhibited the cerulein-induced acinar cell death, cytokine productions, and high-mobility group box 1 expression. Furthermore, LE also inhibited the activation of p38 mitogen-activated protein kinases. CONCLUSIONS: These results suggest that LE plays a protective role during the development of AP by inhibiting the activation of p38. Lippincott Williams & Wilkins 2015-01 2014-12-11 /pmc/articles/PMC4272226/ /pubmed/25102438 http://dx.doi.org/10.1097/MPA.0000000000000195 Text en Copyright © 2014 by Lippincott Williams & Wilkins This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License, where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially.
spellingShingle Original Articles
Choi, Sun Bok
Bae, Gi-Sang
Jo, Il-Joo
Seo, Seung-Hee
Kim, Dong-Goo
Shin, Joon-Yeon
Hong, Seung-Heon
Choi, Byung-Min
Park, Sang-Hyun
Song, Ho-Joon
Park, Sung-Joo
Protective Effects of Lithospermum erythrorhizon Against Cerulein-Induced Acute Pancreatitis
title Protective Effects of Lithospermum erythrorhizon Against Cerulein-Induced Acute Pancreatitis
title_full Protective Effects of Lithospermum erythrorhizon Against Cerulein-Induced Acute Pancreatitis
title_fullStr Protective Effects of Lithospermum erythrorhizon Against Cerulein-Induced Acute Pancreatitis
title_full_unstemmed Protective Effects of Lithospermum erythrorhizon Against Cerulein-Induced Acute Pancreatitis
title_short Protective Effects of Lithospermum erythrorhizon Against Cerulein-Induced Acute Pancreatitis
title_sort protective effects of lithospermum erythrorhizon against cerulein-induced acute pancreatitis
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4272226/
https://www.ncbi.nlm.nih.gov/pubmed/25102438
http://dx.doi.org/10.1097/MPA.0000000000000195
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