An Improved Model for the hTERT Promoter Quadruplex

Mutations occur at four specific sites in the hTERT promoter in >75% of glioblastomas and melanomas, but the mechanism by which the mutations affect gene expression remains unexplained. We report biophysical computational studies that show that the hTERT promoter sequence forms a novel G-quadrupl...

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Detalles Bibliográficos
Autores principales: Chaires, Jonathan B., Trent, John O., Gray, Robert D., Dean, William L., Buscaglia, Robert, Thomas, Shelia D., Miller, Donald M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4272262/
https://www.ncbi.nlm.nih.gov/pubmed/25526084
http://dx.doi.org/10.1371/journal.pone.0115580
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author Chaires, Jonathan B.
Trent, John O.
Gray, Robert D.
Dean, William L.
Buscaglia, Robert
Thomas, Shelia D.
Miller, Donald M.
author_facet Chaires, Jonathan B.
Trent, John O.
Gray, Robert D.
Dean, William L.
Buscaglia, Robert
Thomas, Shelia D.
Miller, Donald M.
author_sort Chaires, Jonathan B.
collection PubMed
description Mutations occur at four specific sites in the hTERT promoter in >75% of glioblastomas and melanomas, but the mechanism by which the mutations affect gene expression remains unexplained. We report biophysical computational studies that show that the hTERT promoter sequence forms a novel G-quadruplex structure consisting of three contiguous, stacked parallel quadruplexes. The reported hTERT mutations map to the central quadruplex within this structure, and lead to an alteration of its hydrodynamic properties and stability.
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spelling pubmed-42722622014-12-26 An Improved Model for the hTERT Promoter Quadruplex Chaires, Jonathan B. Trent, John O. Gray, Robert D. Dean, William L. Buscaglia, Robert Thomas, Shelia D. Miller, Donald M. PLoS One Research Article Mutations occur at four specific sites in the hTERT promoter in >75% of glioblastomas and melanomas, but the mechanism by which the mutations affect gene expression remains unexplained. We report biophysical computational studies that show that the hTERT promoter sequence forms a novel G-quadruplex structure consisting of three contiguous, stacked parallel quadruplexes. The reported hTERT mutations map to the central quadruplex within this structure, and lead to an alteration of its hydrodynamic properties and stability. Public Library of Science 2014-12-19 /pmc/articles/PMC4272262/ /pubmed/25526084 http://dx.doi.org/10.1371/journal.pone.0115580 Text en © 2014 Chaires et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Chaires, Jonathan B.
Trent, John O.
Gray, Robert D.
Dean, William L.
Buscaglia, Robert
Thomas, Shelia D.
Miller, Donald M.
An Improved Model for the hTERT Promoter Quadruplex
title An Improved Model for the hTERT Promoter Quadruplex
title_full An Improved Model for the hTERT Promoter Quadruplex
title_fullStr An Improved Model for the hTERT Promoter Quadruplex
title_full_unstemmed An Improved Model for the hTERT Promoter Quadruplex
title_short An Improved Model for the hTERT Promoter Quadruplex
title_sort improved model for the htert promoter quadruplex
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4272262/
https://www.ncbi.nlm.nih.gov/pubmed/25526084
http://dx.doi.org/10.1371/journal.pone.0115580
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