Cargando…
Genetic analysis and comparative virulence of infectious salmon anemia virus (ISAV) types HPR7a and HPR7b from recent field outbreaks in Chile
BACKGROUND: Infectious salmon anemia (ISA) is a serious disease of marine farmed Atlantic salmon, Salmo salar L. caused by ISA virus (ISAV). ISAV genomic segments 5 and 6 encode surface glycoproteins hemagglutinin-esterase (HE) and F protein important for the pathogenicity of ISAV. In this study, we...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4272776/ https://www.ncbi.nlm.nih.gov/pubmed/25472899 http://dx.doi.org/10.1186/s12985-014-0204-1 |
_version_ | 1782349747071221760 |
---|---|
author | Godoy, Marcos G Suarez, Rudy Lazo, Eduardo S Llegues, Katerina O Kibenge, Molly JT Wang, Yingwei Kibenge, Frederick SB |
author_facet | Godoy, Marcos G Suarez, Rudy Lazo, Eduardo S Llegues, Katerina O Kibenge, Molly JT Wang, Yingwei Kibenge, Frederick SB |
author_sort | Godoy, Marcos G |
collection | PubMed |
description | BACKGROUND: Infectious salmon anemia (ISA) is a serious disease of marine farmed Atlantic salmon, Salmo salar L. caused by ISA virus (ISAV). ISAV genomic segments 5 and 6 encode surface glycoproteins hemagglutinin-esterase (HE) and F protein important for the pathogenicity of ISAV. In this study, we describe the genetic characteristics and relationship between ISAV-HPR7a and ISAV-HPR7b strains that caused the ISA outbreaks in Chile in 2013 and 2014, respectively, and the evolution of the ISAV clades since 2009 based on segment 5 and 6 sequences. METHODS: The study material included samples from six ISA cases in Chile. RNA was extracted from salmon tissues and ISAV isolated from cell culture; segments 5 and 6 were amplified by RT-PCR and compared by alignment with ISAV sequences from the GenBank database. RESULTS: ISAV-HPR7a and ISAV-HPR7b belong to the European Genotype I strains only found in Europe and Chile, and in both cases, show high similarity in segments 5 and 6 with identity between 95–96%. Our data confirm the hypothesis that the original virus was introduced to Chile in 1996. Compared to the 2007 ISAV-HPR7b isolate, the 2014 ISAV-HPR7b does not have an insertion in segment 5 and was associated with low mortality, which suggests that ISAV virulence was attenuated by the absence of the insertion in segment 5. In contrast, the highly virulent ISAV-HPR14 from April 2013 outbreak did not have the insertion in segment 5 either. CONCLUSION: Variability in the ISAV virulence markers supports the quasispecies theory that multiple evolution forces are likely to shape ISAV genetic diversity. Our findings provide evidence of continuing evolution of ISAV in the Chilean aquaculture industry. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12985-014-0204-1) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4272776 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-42727762014-12-22 Genetic analysis and comparative virulence of infectious salmon anemia virus (ISAV) types HPR7a and HPR7b from recent field outbreaks in Chile Godoy, Marcos G Suarez, Rudy Lazo, Eduardo S Llegues, Katerina O Kibenge, Molly JT Wang, Yingwei Kibenge, Frederick SB Virol J Research BACKGROUND: Infectious salmon anemia (ISA) is a serious disease of marine farmed Atlantic salmon, Salmo salar L. caused by ISA virus (ISAV). ISAV genomic segments 5 and 6 encode surface glycoproteins hemagglutinin-esterase (HE) and F protein important for the pathogenicity of ISAV. In this study, we describe the genetic characteristics and relationship between ISAV-HPR7a and ISAV-HPR7b strains that caused the ISA outbreaks in Chile in 2013 and 2014, respectively, and the evolution of the ISAV clades since 2009 based on segment 5 and 6 sequences. METHODS: The study material included samples from six ISA cases in Chile. RNA was extracted from salmon tissues and ISAV isolated from cell culture; segments 5 and 6 were amplified by RT-PCR and compared by alignment with ISAV sequences from the GenBank database. RESULTS: ISAV-HPR7a and ISAV-HPR7b belong to the European Genotype I strains only found in Europe and Chile, and in both cases, show high similarity in segments 5 and 6 with identity between 95–96%. Our data confirm the hypothesis that the original virus was introduced to Chile in 1996. Compared to the 2007 ISAV-HPR7b isolate, the 2014 ISAV-HPR7b does not have an insertion in segment 5 and was associated with low mortality, which suggests that ISAV virulence was attenuated by the absence of the insertion in segment 5. In contrast, the highly virulent ISAV-HPR14 from April 2013 outbreak did not have the insertion in segment 5 either. CONCLUSION: Variability in the ISAV virulence markers supports the quasispecies theory that multiple evolution forces are likely to shape ISAV genetic diversity. Our findings provide evidence of continuing evolution of ISAV in the Chilean aquaculture industry. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12985-014-0204-1) contains supplementary material, which is available to authorized users. BioMed Central 2014-11-29 /pmc/articles/PMC4272776/ /pubmed/25472899 http://dx.doi.org/10.1186/s12985-014-0204-1 Text en © Godoy et al.; licensee BioMed Central Ltd. 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Godoy, Marcos G Suarez, Rudy Lazo, Eduardo S Llegues, Katerina O Kibenge, Molly JT Wang, Yingwei Kibenge, Frederick SB Genetic analysis and comparative virulence of infectious salmon anemia virus (ISAV) types HPR7a and HPR7b from recent field outbreaks in Chile |
title | Genetic analysis and comparative virulence of infectious salmon anemia virus (ISAV) types HPR7a and HPR7b from recent field outbreaks in Chile |
title_full | Genetic analysis and comparative virulence of infectious salmon anemia virus (ISAV) types HPR7a and HPR7b from recent field outbreaks in Chile |
title_fullStr | Genetic analysis and comparative virulence of infectious salmon anemia virus (ISAV) types HPR7a and HPR7b from recent field outbreaks in Chile |
title_full_unstemmed | Genetic analysis and comparative virulence of infectious salmon anemia virus (ISAV) types HPR7a and HPR7b from recent field outbreaks in Chile |
title_short | Genetic analysis and comparative virulence of infectious salmon anemia virus (ISAV) types HPR7a and HPR7b from recent field outbreaks in Chile |
title_sort | genetic analysis and comparative virulence of infectious salmon anemia virus (isav) types hpr7a and hpr7b from recent field outbreaks in chile |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4272776/ https://www.ncbi.nlm.nih.gov/pubmed/25472899 http://dx.doi.org/10.1186/s12985-014-0204-1 |
work_keys_str_mv | AT godoymarcosg geneticanalysisandcomparativevirulenceofinfectioussalmonanemiavirusisavtypeshpr7aandhpr7bfromrecentfieldoutbreaksinchile AT suarezrudy geneticanalysisandcomparativevirulenceofinfectioussalmonanemiavirusisavtypeshpr7aandhpr7bfromrecentfieldoutbreaksinchile AT lazoeduardos geneticanalysisandcomparativevirulenceofinfectioussalmonanemiavirusisavtypeshpr7aandhpr7bfromrecentfieldoutbreaksinchile AT llegueskaterinao geneticanalysisandcomparativevirulenceofinfectioussalmonanemiavirusisavtypeshpr7aandhpr7bfromrecentfieldoutbreaksinchile AT kibengemollyjt geneticanalysisandcomparativevirulenceofinfectioussalmonanemiavirusisavtypeshpr7aandhpr7bfromrecentfieldoutbreaksinchile AT wangyingwei geneticanalysisandcomparativevirulenceofinfectioussalmonanemiavirusisavtypeshpr7aandhpr7bfromrecentfieldoutbreaksinchile AT kibengefredericksb geneticanalysisandcomparativevirulenceofinfectioussalmonanemiavirusisavtypeshpr7aandhpr7bfromrecentfieldoutbreaksinchile |