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The role of microRNA-133b and its target gene FSCN1 in gastric cancer
BACKGROUND: Increasing evidences have documented that microRNAs (miRNAs) act as oncogenes or tumor suppressors in gastric cancer (GC). In this study, we aimed to investigate the expression of miR-133b in a large number of GC samples and elucidate its role in GC carcinogenesis and the detailed mechan...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4272783/ https://www.ncbi.nlm.nih.gov/pubmed/25433493 http://dx.doi.org/10.1186/s13046-014-0099-0 |
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author | Guo, Lihua Bai, Hua Zou, Dongling Hong, Tao Liu, Jie Huang, Jiaqiang He, Pengfei Zhou, Qi He, Jinsheng |
author_facet | Guo, Lihua Bai, Hua Zou, Dongling Hong, Tao Liu, Jie Huang, Jiaqiang He, Pengfei Zhou, Qi He, Jinsheng |
author_sort | Guo, Lihua |
collection | PubMed |
description | BACKGROUND: Increasing evidences have documented that microRNAs (miRNAs) act as oncogenes or tumor suppressors in gastric cancer (GC). In this study, we aimed to investigate the expression of miR-133b in a large number of GC samples and elucidate its role in GC carcinogenesis and the detailed mechanism. METHODS: We used Taqman probe stem-loop real-time PCR to accurately measure the levels of miR-133b in 100 pairs of gastric cancer tissues and the adjacent non-neoplastic tissues. miR-133b mimics were overexpressed in GC cell lines, miR-133b inhibitors were also introduced in GES cells to investigate its role on regulating cell proliferation, cell migration and cell invasion. The target of miR-133b was identified by luciferase reporter assay and western blot. Fascin actin-bundling protein 1 (FSCN1) siRNA was used to achieve the knockdown of FSCN1 in GC cells and to investigate its role on modulating GC cell proliferation and invasion. RESULTS: miR-133b was significantly down-regulated in GC cell lines and in GC tissues compared with adjacent normal tissues. Moreover, lower-level of miR-133b was also associated with venous invasion and a more aggressive tumor phenotype. Re-introduction of miR-133b in GC cells can inhibit cell proliferation, cell migration and invasion. In contrary, knockdown of miR-133b in GES cells can promote cell proliferation and invasion. Further investigation indicated that miR-133b targeted FSCN1 in GC cells and knockdown of FSCN1 can also inhibit GC cell growth and invasion. CONCLUSION: Our findings demonstrated that miR-133b was significantly down-regulated in GC tissues and exerted its tumor suppressor role in GC cells. The investigation of the detailed mechanism showed that miR-133b directly targeted FSCN1 which functioned as an oncogenic gene in GC cells. These results suggested that miR-133b can be developed as a new diagnostic marker or therapeutic target for GC. |
format | Online Article Text |
id | pubmed-4272783 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-42727832014-12-22 The role of microRNA-133b and its target gene FSCN1 in gastric cancer Guo, Lihua Bai, Hua Zou, Dongling Hong, Tao Liu, Jie Huang, Jiaqiang He, Pengfei Zhou, Qi He, Jinsheng J Exp Clin Cancer Res Research BACKGROUND: Increasing evidences have documented that microRNAs (miRNAs) act as oncogenes or tumor suppressors in gastric cancer (GC). In this study, we aimed to investigate the expression of miR-133b in a large number of GC samples and elucidate its role in GC carcinogenesis and the detailed mechanism. METHODS: We used Taqman probe stem-loop real-time PCR to accurately measure the levels of miR-133b in 100 pairs of gastric cancer tissues and the adjacent non-neoplastic tissues. miR-133b mimics were overexpressed in GC cell lines, miR-133b inhibitors were also introduced in GES cells to investigate its role on regulating cell proliferation, cell migration and cell invasion. The target of miR-133b was identified by luciferase reporter assay and western blot. Fascin actin-bundling protein 1 (FSCN1) siRNA was used to achieve the knockdown of FSCN1 in GC cells and to investigate its role on modulating GC cell proliferation and invasion. RESULTS: miR-133b was significantly down-regulated in GC cell lines and in GC tissues compared with adjacent normal tissues. Moreover, lower-level of miR-133b was also associated with venous invasion and a more aggressive tumor phenotype. Re-introduction of miR-133b in GC cells can inhibit cell proliferation, cell migration and invasion. In contrary, knockdown of miR-133b in GES cells can promote cell proliferation and invasion. Further investigation indicated that miR-133b targeted FSCN1 in GC cells and knockdown of FSCN1 can also inhibit GC cell growth and invasion. CONCLUSION: Our findings demonstrated that miR-133b was significantly down-regulated in GC tissues and exerted its tumor suppressor role in GC cells. The investigation of the detailed mechanism showed that miR-133b directly targeted FSCN1 which functioned as an oncogenic gene in GC cells. These results suggested that miR-133b can be developed as a new diagnostic marker or therapeutic target for GC. BioMed Central 2014-11-30 /pmc/articles/PMC4272783/ /pubmed/25433493 http://dx.doi.org/10.1186/s13046-014-0099-0 Text en © Guo et al.; licensee BioMed Central Ltd. 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Guo, Lihua Bai, Hua Zou, Dongling Hong, Tao Liu, Jie Huang, Jiaqiang He, Pengfei Zhou, Qi He, Jinsheng The role of microRNA-133b and its target gene FSCN1 in gastric cancer |
title | The role of microRNA-133b and its target gene FSCN1 in gastric cancer |
title_full | The role of microRNA-133b and its target gene FSCN1 in gastric cancer |
title_fullStr | The role of microRNA-133b and its target gene FSCN1 in gastric cancer |
title_full_unstemmed | The role of microRNA-133b and its target gene FSCN1 in gastric cancer |
title_short | The role of microRNA-133b and its target gene FSCN1 in gastric cancer |
title_sort | role of microrna-133b and its target gene fscn1 in gastric cancer |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4272783/ https://www.ncbi.nlm.nih.gov/pubmed/25433493 http://dx.doi.org/10.1186/s13046-014-0099-0 |
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