Cargando…
Correlation between SD-OCT, immunocytochemistry and functional findings in an animal model of retinal degeneration
Purpose: The P23H rhodopsin mutation is an autosomal dominant cause of retinitis pigmentosa (RP). The degeneration can be tracked using different anatomical and functional methods. In our case, we evaluated the anatomical changes using Spectral-Domain Optical Coherence Tomography (SD-OCT) and correl...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4273614/ https://www.ncbi.nlm.nih.gov/pubmed/25565976 http://dx.doi.org/10.3389/fnana.2014.00151 |
_version_ | 1782349864719351808 |
---|---|
author | Cuenca, Nicolás Fernández-Sánchez, Laura Sauvé, Yves Segura, Francisco J. Martínez-Navarrete, Gema Tamarit, José Manuel Fuentes-Broto, Lorena Sanchez-Cano, Ana Pinilla, Isabel |
author_facet | Cuenca, Nicolás Fernández-Sánchez, Laura Sauvé, Yves Segura, Francisco J. Martínez-Navarrete, Gema Tamarit, José Manuel Fuentes-Broto, Lorena Sanchez-Cano, Ana Pinilla, Isabel |
author_sort | Cuenca, Nicolás |
collection | PubMed |
description | Purpose: The P23H rhodopsin mutation is an autosomal dominant cause of retinitis pigmentosa (RP). The degeneration can be tracked using different anatomical and functional methods. In our case, we evaluated the anatomical changes using Spectral-Domain Optical Coherence Tomography (SD-OCT) and correlated the findings with retinal thickness values determined by immunocytochemistry.Methods: Pigmented rats heterozygous for the P23H mutation, with ages between P18 and P180 were studied. Function was assessed by means of optomotor testing and ERGs. Retinal thicknesses measurements, autofluorescence and fluorescein angiography were performed using Spectralis OCT. Retinas were studied by means of immunohistochemistry. Results: Between P30 and P180, visual acuity decreased from 0.500 to 0.182 cycles per degree (cyc/deg) and contrast sensitivity decreased from 54.56 to 2.98 for a spatial frequency of 0.089 cyc/deg. Only cone-driven b-wave responses reached developmental maturity. Flicker fusions were also comparable at P29 (42 Hz). Double flash-isolated rod-driven responses were already affected at P29. Photopic responses revealed deterioration after P29.A reduction in retinal thicknesses and morphological modifications were seen in OCT sections. Statistically significant differences were found in all evaluated thicknesses. Autofluorescence was seen in P23H rats as sparse dots. Immunocytochemistry showed a progressive decrease in the outer nuclear layer (ONL), and morphological changes. Although anatomical thickness measures were significantly lower than OCT values, there was a very strong correlation between the values measured by both techniques.Conclusions: In pigmented P23H rats, a progressive deterioration occurs in both retinal function and anatomy. Anatomical changes can be effectively evaluated using SD-OCT and immunocytochemistry, with a good correlation between their values, thus making SD-OCT an important tool for research in retinal degeneration. |
format | Online Article Text |
id | pubmed-4273614 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-42736142015-01-06 Correlation between SD-OCT, immunocytochemistry and functional findings in an animal model of retinal degeneration Cuenca, Nicolás Fernández-Sánchez, Laura Sauvé, Yves Segura, Francisco J. Martínez-Navarrete, Gema Tamarit, José Manuel Fuentes-Broto, Lorena Sanchez-Cano, Ana Pinilla, Isabel Front Neuroanat Neuroscience Purpose: The P23H rhodopsin mutation is an autosomal dominant cause of retinitis pigmentosa (RP). The degeneration can be tracked using different anatomical and functional methods. In our case, we evaluated the anatomical changes using Spectral-Domain Optical Coherence Tomography (SD-OCT) and correlated the findings with retinal thickness values determined by immunocytochemistry.Methods: Pigmented rats heterozygous for the P23H mutation, with ages between P18 and P180 were studied. Function was assessed by means of optomotor testing and ERGs. Retinal thicknesses measurements, autofluorescence and fluorescein angiography were performed using Spectralis OCT. Retinas were studied by means of immunohistochemistry. Results: Between P30 and P180, visual acuity decreased from 0.500 to 0.182 cycles per degree (cyc/deg) and contrast sensitivity decreased from 54.56 to 2.98 for a spatial frequency of 0.089 cyc/deg. Only cone-driven b-wave responses reached developmental maturity. Flicker fusions were also comparable at P29 (42 Hz). Double flash-isolated rod-driven responses were already affected at P29. Photopic responses revealed deterioration after P29.A reduction in retinal thicknesses and morphological modifications were seen in OCT sections. Statistically significant differences were found in all evaluated thicknesses. Autofluorescence was seen in P23H rats as sparse dots. Immunocytochemistry showed a progressive decrease in the outer nuclear layer (ONL), and morphological changes. Although anatomical thickness measures were significantly lower than OCT values, there was a very strong correlation between the values measured by both techniques.Conclusions: In pigmented P23H rats, a progressive deterioration occurs in both retinal function and anatomy. Anatomical changes can be effectively evaluated using SD-OCT and immunocytochemistry, with a good correlation between their values, thus making SD-OCT an important tool for research in retinal degeneration. Frontiers Media S.A. 2014-12-22 /pmc/articles/PMC4273614/ /pubmed/25565976 http://dx.doi.org/10.3389/fnana.2014.00151 Text en Copyright © 2014 Cuenca, Fernández-Sánchez, Sauvé, Segura, Martínez-Navarrete, Tamarit, Fuentes-Broto, Sanchez-Cano and Pinilla. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Cuenca, Nicolás Fernández-Sánchez, Laura Sauvé, Yves Segura, Francisco J. Martínez-Navarrete, Gema Tamarit, José Manuel Fuentes-Broto, Lorena Sanchez-Cano, Ana Pinilla, Isabel Correlation between SD-OCT, immunocytochemistry and functional findings in an animal model of retinal degeneration |
title | Correlation between SD-OCT, immunocytochemistry and functional findings in an animal model of retinal degeneration |
title_full | Correlation between SD-OCT, immunocytochemistry and functional findings in an animal model of retinal degeneration |
title_fullStr | Correlation between SD-OCT, immunocytochemistry and functional findings in an animal model of retinal degeneration |
title_full_unstemmed | Correlation between SD-OCT, immunocytochemistry and functional findings in an animal model of retinal degeneration |
title_short | Correlation between SD-OCT, immunocytochemistry and functional findings in an animal model of retinal degeneration |
title_sort | correlation between sd-oct, immunocytochemistry and functional findings in an animal model of retinal degeneration |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4273614/ https://www.ncbi.nlm.nih.gov/pubmed/25565976 http://dx.doi.org/10.3389/fnana.2014.00151 |
work_keys_str_mv | AT cuencanicolas correlationbetweensdoctimmunocytochemistryandfunctionalfindingsinananimalmodelofretinaldegeneration AT fernandezsanchezlaura correlationbetweensdoctimmunocytochemistryandfunctionalfindingsinananimalmodelofretinaldegeneration AT sauveyves correlationbetweensdoctimmunocytochemistryandfunctionalfindingsinananimalmodelofretinaldegeneration AT segurafranciscoj correlationbetweensdoctimmunocytochemistryandfunctionalfindingsinananimalmodelofretinaldegeneration AT martineznavarretegema correlationbetweensdoctimmunocytochemistryandfunctionalfindingsinananimalmodelofretinaldegeneration AT tamaritjosemanuel correlationbetweensdoctimmunocytochemistryandfunctionalfindingsinananimalmodelofretinaldegeneration AT fuentesbrotolorena correlationbetweensdoctimmunocytochemistryandfunctionalfindingsinananimalmodelofretinaldegeneration AT sanchezcanoana correlationbetweensdoctimmunocytochemistryandfunctionalfindingsinananimalmodelofretinaldegeneration AT pinillaisabel correlationbetweensdoctimmunocytochemistryandfunctionalfindingsinananimalmodelofretinaldegeneration |