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Fold or hold: experimental evolution in vitro

We introduce a system for experimental evolution consisting of populations of short oligonucleotides (Oli populations) evolving in a modified quantitative polymerase chain reaction (qPCR). It is tractable at the genetic, genomic, phenotypic and fitness levels. The Oli system uses DNA hairpins design...

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Detalles Bibliográficos
Autores principales: Collins, S, Rambaut, A, Bridgett, S J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4274015/
https://www.ncbi.nlm.nih.gov/pubmed/24003997
http://dx.doi.org/10.1111/jeb.12233
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author Collins, S
Rambaut, A
Bridgett, S J
author_facet Collins, S
Rambaut, A
Bridgett, S J
author_sort Collins, S
collection PubMed
description We introduce a system for experimental evolution consisting of populations of short oligonucleotides (Oli populations) evolving in a modified quantitative polymerase chain reaction (qPCR). It is tractable at the genetic, genomic, phenotypic and fitness levels. The Oli system uses DNA hairpins designed to form structures that self-prime under defined conditions. Selection acts on the phenotype of self-priming, after which differences in fitness are amplified and quantified using qPCR. We outline the methodological and bioinformatics tools for the Oli system here and demonstrate that it can be used as a conventional experimental evolution model system by test-driving it in an experiment investigating adaptive evolution under different rates of environmental change.
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spelling pubmed-42740152014-12-23 Fold or hold: experimental evolution in vitro Collins, S Rambaut, A Bridgett, S J J Evol Biol Research Papers We introduce a system for experimental evolution consisting of populations of short oligonucleotides (Oli populations) evolving in a modified quantitative polymerase chain reaction (qPCR). It is tractable at the genetic, genomic, phenotypic and fitness levels. The Oli system uses DNA hairpins designed to form structures that self-prime under defined conditions. Selection acts on the phenotype of self-priming, after which differences in fitness are amplified and quantified using qPCR. We outline the methodological and bioinformatics tools for the Oli system here and demonstrate that it can be used as a conventional experimental evolution model system by test-driving it in an experiment investigating adaptive evolution under different rates of environmental change. BlackWell Publishing Ltd 2013-10 2013-09-04 /pmc/articles/PMC4274015/ /pubmed/24003997 http://dx.doi.org/10.1111/jeb.12233 Text en © 2013 The Authors. Journal of Evolutionary Biology © 2013 European Society For Evolutionary Biology http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
Collins, S
Rambaut, A
Bridgett, S J
Fold or hold: experimental evolution in vitro
title Fold or hold: experimental evolution in vitro
title_full Fold or hold: experimental evolution in vitro
title_fullStr Fold or hold: experimental evolution in vitro
title_full_unstemmed Fold or hold: experimental evolution in vitro
title_short Fold or hold: experimental evolution in vitro
title_sort fold or hold: experimental evolution in vitro
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4274015/
https://www.ncbi.nlm.nih.gov/pubmed/24003997
http://dx.doi.org/10.1111/jeb.12233
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