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Remodeling of secretory compartments creates CUPS during nutrient starvation

Upon starvation, Grh1, a peripheral membrane protein located at endoplasmic reticulum (ER) exit sites and early Golgi in Saccharomyces cerevisiae under growth conditions, relocates to a compartment called compartment for unconventional protein secretion (CUPS). Here we report that CUPS lack Golgi en...

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Autores principales: Cruz-Garcia, David, Curwin, Amy J., Popoff, Jean-François, Bruns, Caroline, Duran, Juan M., Malhotra, Vivek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4274258/
https://www.ncbi.nlm.nih.gov/pubmed/25512390
http://dx.doi.org/10.1083/jcb.201407119
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author Cruz-Garcia, David
Curwin, Amy J.
Popoff, Jean-François
Bruns, Caroline
Duran, Juan M.
Malhotra, Vivek
author_facet Cruz-Garcia, David
Curwin, Amy J.
Popoff, Jean-François
Bruns, Caroline
Duran, Juan M.
Malhotra, Vivek
author_sort Cruz-Garcia, David
collection PubMed
description Upon starvation, Grh1, a peripheral membrane protein located at endoplasmic reticulum (ER) exit sites and early Golgi in Saccharomyces cerevisiae under growth conditions, relocates to a compartment called compartment for unconventional protein secretion (CUPS). Here we report that CUPS lack Golgi enzymes, but contain the coat protein complex II (COPII) vesicle tethering protein Uso1 and the Golgi t-SNARE Sed5. Interestingly, CUPS biogenesis is independent of COPII- and COPI-mediated membrane transport. Pik1- and Sec7-mediated membrane export from the late Golgi is required for complete assembly of CUPS, and Vps34 is needed for their maintenance. CUPS formation is triggered by glucose, but not nitrogen starvation. Moreover, upon return to growth conditions, CUPS are absorbed into the ER, and not the vacuole. Altogether our findings indicate that CUPS are not specialized autophagosomes as suggested previously. We suggest that starvation triggers relocation of secretory and endosomal membranes, but not their enzymes, to generate CUPS to sort and secrete proteins that do not enter, or are not processed by enzymes of the ER–Golgi pathway of secretion.
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spelling pubmed-42742582015-06-22 Remodeling of secretory compartments creates CUPS during nutrient starvation Cruz-Garcia, David Curwin, Amy J. Popoff, Jean-François Bruns, Caroline Duran, Juan M. Malhotra, Vivek J Cell Biol Research Articles Upon starvation, Grh1, a peripheral membrane protein located at endoplasmic reticulum (ER) exit sites and early Golgi in Saccharomyces cerevisiae under growth conditions, relocates to a compartment called compartment for unconventional protein secretion (CUPS). Here we report that CUPS lack Golgi enzymes, but contain the coat protein complex II (COPII) vesicle tethering protein Uso1 and the Golgi t-SNARE Sed5. Interestingly, CUPS biogenesis is independent of COPII- and COPI-mediated membrane transport. Pik1- and Sec7-mediated membrane export from the late Golgi is required for complete assembly of CUPS, and Vps34 is needed for their maintenance. CUPS formation is triggered by glucose, but not nitrogen starvation. Moreover, upon return to growth conditions, CUPS are absorbed into the ER, and not the vacuole. Altogether our findings indicate that CUPS are not specialized autophagosomes as suggested previously. We suggest that starvation triggers relocation of secretory and endosomal membranes, but not their enzymes, to generate CUPS to sort and secrete proteins that do not enter, or are not processed by enzymes of the ER–Golgi pathway of secretion. The Rockefeller University Press 2014-12-22 /pmc/articles/PMC4274258/ /pubmed/25512390 http://dx.doi.org/10.1083/jcb.201407119 Text en © 2014 Cruz-Garcia et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Cruz-Garcia, David
Curwin, Amy J.
Popoff, Jean-François
Bruns, Caroline
Duran, Juan M.
Malhotra, Vivek
Remodeling of secretory compartments creates CUPS during nutrient starvation
title Remodeling of secretory compartments creates CUPS during nutrient starvation
title_full Remodeling of secretory compartments creates CUPS during nutrient starvation
title_fullStr Remodeling of secretory compartments creates CUPS during nutrient starvation
title_full_unstemmed Remodeling of secretory compartments creates CUPS during nutrient starvation
title_short Remodeling of secretory compartments creates CUPS during nutrient starvation
title_sort remodeling of secretory compartments creates cups during nutrient starvation
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4274258/
https://www.ncbi.nlm.nih.gov/pubmed/25512390
http://dx.doi.org/10.1083/jcb.201407119
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