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Uncovering the cathepsin system in heart failure patients submitted to Left Ventricular Assist Device (LVAD) implantation

BACKGROUND: In end-stage heart failure (HF), the implantation of a left ventricular assist device (LVAD) is able to induce reverse remodeling. Cellular proteases, such as cathepsins, are involved in the progression of HF. The aim of this study was to evaluate the role of cathepsin system in HF patie...

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Autores principales: D’Amico, Andrea, Ragusa, Rosetta, Caruso, Raffaele, Prescimone, Tommaso, Nonini, Sandra, Cabiati, Manuela, Del Ry, Silvia, Trivella, Maria Giovanna, Giannessi, Daniela, Caselli, Chiara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4274696/
https://www.ncbi.nlm.nih.gov/pubmed/25496327
http://dx.doi.org/10.1186/s12967-014-0350-7
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author D’Amico, Andrea
Ragusa, Rosetta
Caruso, Raffaele
Prescimone, Tommaso
Nonini, Sandra
Cabiati, Manuela
Del Ry, Silvia
Trivella, Maria Giovanna
Giannessi, Daniela
Caselli, Chiara
author_facet D’Amico, Andrea
Ragusa, Rosetta
Caruso, Raffaele
Prescimone, Tommaso
Nonini, Sandra
Cabiati, Manuela
Del Ry, Silvia
Trivella, Maria Giovanna
Giannessi, Daniela
Caselli, Chiara
author_sort D’Amico, Andrea
collection PubMed
description BACKGROUND: In end-stage heart failure (HF), the implantation of a left ventricular assist device (LVAD) is able to induce reverse remodeling. Cellular proteases, such as cathepsins, are involved in the progression of HF. The aim of this study was to evaluate the role of cathepsin system in HF patients supported by LVAD, in order to determine their involvement in cardiac remodeling. METHODS: The expression of cysteine (CatB, CatK, CatL, CatS) and serine cathepsin (CatG), and relative inhibitors (Cystatin B, C and SerpinA3, respectively) was determined in cardiac biopsies of 22 patients submitted to LVAD (pre-LVAD) and compared with: 1) control stable chronic HF patients on medical therapy at the moment of heart transplantation without prior LVAD (HT, n = 7); 2) patients supported by LVAD at the moment of transplantation (post-LVAD, n = 6). RESULTS: The expression of cathepsins and their inhibitors was significantly higher in pre-LVAD compared to the HT group and LVAD induced a further increase in the cathepsin system. Significant positive correlations were observed between cardiac expression of cathepsins and their inhibitors as well as inflammatory cytokines. In the pre-LVAD group, a relationship of cathepsins with dilatative etiology and length of hospitalization was found. CONCLUSIONS: A parallel activation of cathepsins and their inhibitors was observed after LVAD support. The possible clinical importance of these modifications is confirmed by their relation with patients’ outcome. A better discovery of these pathways could add more insights into the cardiac remodeling during HF.
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spelling pubmed-42746962014-12-24 Uncovering the cathepsin system in heart failure patients submitted to Left Ventricular Assist Device (LVAD) implantation D’Amico, Andrea Ragusa, Rosetta Caruso, Raffaele Prescimone, Tommaso Nonini, Sandra Cabiati, Manuela Del Ry, Silvia Trivella, Maria Giovanna Giannessi, Daniela Caselli, Chiara J Transl Med Research BACKGROUND: In end-stage heart failure (HF), the implantation of a left ventricular assist device (LVAD) is able to induce reverse remodeling. Cellular proteases, such as cathepsins, are involved in the progression of HF. The aim of this study was to evaluate the role of cathepsin system in HF patients supported by LVAD, in order to determine their involvement in cardiac remodeling. METHODS: The expression of cysteine (CatB, CatK, CatL, CatS) and serine cathepsin (CatG), and relative inhibitors (Cystatin B, C and SerpinA3, respectively) was determined in cardiac biopsies of 22 patients submitted to LVAD (pre-LVAD) and compared with: 1) control stable chronic HF patients on medical therapy at the moment of heart transplantation without prior LVAD (HT, n = 7); 2) patients supported by LVAD at the moment of transplantation (post-LVAD, n = 6). RESULTS: The expression of cathepsins and their inhibitors was significantly higher in pre-LVAD compared to the HT group and LVAD induced a further increase in the cathepsin system. Significant positive correlations were observed between cardiac expression of cathepsins and their inhibitors as well as inflammatory cytokines. In the pre-LVAD group, a relationship of cathepsins with dilatative etiology and length of hospitalization was found. CONCLUSIONS: A parallel activation of cathepsins and their inhibitors was observed after LVAD support. The possible clinical importance of these modifications is confirmed by their relation with patients’ outcome. A better discovery of these pathways could add more insights into the cardiac remodeling during HF. BioMed Central 2014-12-12 /pmc/articles/PMC4274696/ /pubmed/25496327 http://dx.doi.org/10.1186/s12967-014-0350-7 Text en © D'Amico et al.; licensee BioMed Central. 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
D’Amico, Andrea
Ragusa, Rosetta
Caruso, Raffaele
Prescimone, Tommaso
Nonini, Sandra
Cabiati, Manuela
Del Ry, Silvia
Trivella, Maria Giovanna
Giannessi, Daniela
Caselli, Chiara
Uncovering the cathepsin system in heart failure patients submitted to Left Ventricular Assist Device (LVAD) implantation
title Uncovering the cathepsin system in heart failure patients submitted to Left Ventricular Assist Device (LVAD) implantation
title_full Uncovering the cathepsin system in heart failure patients submitted to Left Ventricular Assist Device (LVAD) implantation
title_fullStr Uncovering the cathepsin system in heart failure patients submitted to Left Ventricular Assist Device (LVAD) implantation
title_full_unstemmed Uncovering the cathepsin system in heart failure patients submitted to Left Ventricular Assist Device (LVAD) implantation
title_short Uncovering the cathepsin system in heart failure patients submitted to Left Ventricular Assist Device (LVAD) implantation
title_sort uncovering the cathepsin system in heart failure patients submitted to left ventricular assist device (lvad) implantation
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4274696/
https://www.ncbi.nlm.nih.gov/pubmed/25496327
http://dx.doi.org/10.1186/s12967-014-0350-7
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