Cargando…
Chemical strategies for the covalent modification of filamentous phage
Historically filamentous bacteriophage have been known to be the workhorse of phage display due to their ability to link genotype to phenotype. More recently, the filamentous phage scaffold has proven to be powerful outside the realm of phage display technology in fields such as molecular imaging, c...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4274979/ https://www.ncbi.nlm.nih.gov/pubmed/25566240 http://dx.doi.org/10.3389/fmicb.2014.00734 |
_version_ | 1782350069506244608 |
---|---|
author | Bernard, Jenna M. L. Francis, Matthew B. |
author_facet | Bernard, Jenna M. L. Francis, Matthew B. |
author_sort | Bernard, Jenna M. L. |
collection | PubMed |
description | Historically filamentous bacteriophage have been known to be the workhorse of phage display due to their ability to link genotype to phenotype. More recently, the filamentous phage scaffold has proven to be powerful outside the realm of phage display technology in fields such as molecular imaging, cancer research and materials, and vaccine development. The ability of the virion to serve as a platform for a variety of applications heavily relies on the functionalization of the phage coat proteins with a wide variety of functionalities. Genetic modification of the coat proteins has been the most widely used strategy for functionalizing the virion; however, complementary chemical modification strategies can help to diversify the range of materials that can be developed. This review emphasizes the recent advances that have been made in the chemical modification of filamentous phage as well as some of the challenges that are involved in functionalizing the virion. |
format | Online Article Text |
id | pubmed-4274979 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-42749792015-01-06 Chemical strategies for the covalent modification of filamentous phage Bernard, Jenna M. L. Francis, Matthew B. Front Microbiol Microbiology Historically filamentous bacteriophage have been known to be the workhorse of phage display due to their ability to link genotype to phenotype. More recently, the filamentous phage scaffold has proven to be powerful outside the realm of phage display technology in fields such as molecular imaging, cancer research and materials, and vaccine development. The ability of the virion to serve as a platform for a variety of applications heavily relies on the functionalization of the phage coat proteins with a wide variety of functionalities. Genetic modification of the coat proteins has been the most widely used strategy for functionalizing the virion; however, complementary chemical modification strategies can help to diversify the range of materials that can be developed. This review emphasizes the recent advances that have been made in the chemical modification of filamentous phage as well as some of the challenges that are involved in functionalizing the virion. Frontiers Media S.A. 2014-12-23 /pmc/articles/PMC4274979/ /pubmed/25566240 http://dx.doi.org/10.3389/fmicb.2014.00734 Text en Copyright © 2014 Bernard and Francis. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Bernard, Jenna M. L. Francis, Matthew B. Chemical strategies for the covalent modification of filamentous phage |
title | Chemical strategies for the covalent modification of filamentous phage |
title_full | Chemical strategies for the covalent modification of filamentous phage |
title_fullStr | Chemical strategies for the covalent modification of filamentous phage |
title_full_unstemmed | Chemical strategies for the covalent modification of filamentous phage |
title_short | Chemical strategies for the covalent modification of filamentous phage |
title_sort | chemical strategies for the covalent modification of filamentous phage |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4274979/ https://www.ncbi.nlm.nih.gov/pubmed/25566240 http://dx.doi.org/10.3389/fmicb.2014.00734 |
work_keys_str_mv | AT bernardjennaml chemicalstrategiesforthecovalentmodificationoffilamentousphage AT francismatthewb chemicalstrategiesforthecovalentmodificationoffilamentousphage |