Cargando…

Overexpression of CD90 (Thy-1) in Pancreatic Adenocarcinoma Present in the Tumor Microenvironment

CD90 (Thy-1) plays important roles in oncogenesis and shows potential as a candidate marker for cancer stem cells (CSCs) in various malignancies. Herein, we investigated the expression of CD90 in pancreatic adenocarcinoma (PDAC), with a comparison to normal pancreas and non-malignant pancreatic dise...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhu, Jianhui, Thakolwiboon, Smathorn, Liu, Xinhua, Zhang, Min, Lubman, David M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4275230/
https://www.ncbi.nlm.nih.gov/pubmed/25536077
http://dx.doi.org/10.1371/journal.pone.0115507
_version_ 1782350100976107520
author Zhu, Jianhui
Thakolwiboon, Smathorn
Liu, Xinhua
Zhang, Min
Lubman, David M.
author_facet Zhu, Jianhui
Thakolwiboon, Smathorn
Liu, Xinhua
Zhang, Min
Lubman, David M.
author_sort Zhu, Jianhui
collection PubMed
description CD90 (Thy-1) plays important roles in oncogenesis and shows potential as a candidate marker for cancer stem cells (CSCs) in various malignancies. Herein, we investigated the expression of CD90 in pancreatic adenocarcinoma (PDAC), with a comparison to normal pancreas and non-malignant pancreatic disease, by immunohistochemical (IHC) analysis of tissue microarrays containing 183 clinical tissue specimens. Statistical analysis was performed to evaluate the correlation between CD90 expression and the major clinicopathological factors after adjustment of age and gender. The IHC data showed that CD90 was significantly overexpressed in PDAC and its metastatic cancers as compared to chronic pancreatitis and benign islet tumors, while it was negative in normal pancreas and 82.7% of adjacent normal pancreas tissues. The abundant CD90 expression was predominantly present in PDAC stroma, such as fibroblasts and vascular endothelial cells, which could serve as a promising marker to distinguish pancreatic adenocarcinoma from normal pancreas and non-malignant pancreatic diseases. Double immunostaining of CD90 with CD24, a CSC marker for PDAC, showed that there was little overlap between these two markers. However, CD90(+) fibroblast cells were clustered around CD24(+) malignant ducts, suggesting that CD90 may be involved in the tumor-stroma interactions and promote pancreatic cancer development. Furthermore, CD90 mostly overlapped with α-smooth muscle actin (αSMA, a marker of activated pancreatic stellate cells (PSCs)) in PDAC stroma, which demonstrated that CD90(+) stromal cells consist largely of activated PSCs. Double immunostaining of CD90 and a vascular endothelial cell marker CD31 demonstrated that CD90 expression on vascular endothelial cells was significantly increased in PDACs as compared to normal pancreas and non-malignant pancreatic diseases. Our findings suggest that CD90 could serve as a promising marker for pancreatic adenocarcinoma where desmoplastic stroma plays an important role in tumor growth and angiogenesis.
format Online
Article
Text
id pubmed-4275230
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-42752302014-12-31 Overexpression of CD90 (Thy-1) in Pancreatic Adenocarcinoma Present in the Tumor Microenvironment Zhu, Jianhui Thakolwiboon, Smathorn Liu, Xinhua Zhang, Min Lubman, David M. PLoS One Research Article CD90 (Thy-1) plays important roles in oncogenesis and shows potential as a candidate marker for cancer stem cells (CSCs) in various malignancies. Herein, we investigated the expression of CD90 in pancreatic adenocarcinoma (PDAC), with a comparison to normal pancreas and non-malignant pancreatic disease, by immunohistochemical (IHC) analysis of tissue microarrays containing 183 clinical tissue specimens. Statistical analysis was performed to evaluate the correlation between CD90 expression and the major clinicopathological factors after adjustment of age and gender. The IHC data showed that CD90 was significantly overexpressed in PDAC and its metastatic cancers as compared to chronic pancreatitis and benign islet tumors, while it was negative in normal pancreas and 82.7% of adjacent normal pancreas tissues. The abundant CD90 expression was predominantly present in PDAC stroma, such as fibroblasts and vascular endothelial cells, which could serve as a promising marker to distinguish pancreatic adenocarcinoma from normal pancreas and non-malignant pancreatic diseases. Double immunostaining of CD90 with CD24, a CSC marker for PDAC, showed that there was little overlap between these two markers. However, CD90(+) fibroblast cells were clustered around CD24(+) malignant ducts, suggesting that CD90 may be involved in the tumor-stroma interactions and promote pancreatic cancer development. Furthermore, CD90 mostly overlapped with α-smooth muscle actin (αSMA, a marker of activated pancreatic stellate cells (PSCs)) in PDAC stroma, which demonstrated that CD90(+) stromal cells consist largely of activated PSCs. Double immunostaining of CD90 and a vascular endothelial cell marker CD31 demonstrated that CD90 expression on vascular endothelial cells was significantly increased in PDACs as compared to normal pancreas and non-malignant pancreatic diseases. Our findings suggest that CD90 could serve as a promising marker for pancreatic adenocarcinoma where desmoplastic stroma plays an important role in tumor growth and angiogenesis. Public Library of Science 2014-12-23 /pmc/articles/PMC4275230/ /pubmed/25536077 http://dx.doi.org/10.1371/journal.pone.0115507 Text en © 2014 Zhu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zhu, Jianhui
Thakolwiboon, Smathorn
Liu, Xinhua
Zhang, Min
Lubman, David M.
Overexpression of CD90 (Thy-1) in Pancreatic Adenocarcinoma Present in the Tumor Microenvironment
title Overexpression of CD90 (Thy-1) in Pancreatic Adenocarcinoma Present in the Tumor Microenvironment
title_full Overexpression of CD90 (Thy-1) in Pancreatic Adenocarcinoma Present in the Tumor Microenvironment
title_fullStr Overexpression of CD90 (Thy-1) in Pancreatic Adenocarcinoma Present in the Tumor Microenvironment
title_full_unstemmed Overexpression of CD90 (Thy-1) in Pancreatic Adenocarcinoma Present in the Tumor Microenvironment
title_short Overexpression of CD90 (Thy-1) in Pancreatic Adenocarcinoma Present in the Tumor Microenvironment
title_sort overexpression of cd90 (thy-1) in pancreatic adenocarcinoma present in the tumor microenvironment
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4275230/
https://www.ncbi.nlm.nih.gov/pubmed/25536077
http://dx.doi.org/10.1371/journal.pone.0115507
work_keys_str_mv AT zhujianhui overexpressionofcd90thy1inpancreaticadenocarcinomapresentinthetumormicroenvironment
AT thakolwiboonsmathorn overexpressionofcd90thy1inpancreaticadenocarcinomapresentinthetumormicroenvironment
AT liuxinhua overexpressionofcd90thy1inpancreaticadenocarcinomapresentinthetumormicroenvironment
AT zhangmin overexpressionofcd90thy1inpancreaticadenocarcinomapresentinthetumormicroenvironment
AT lubmandavidm overexpressionofcd90thy1inpancreaticadenocarcinomapresentinthetumormicroenvironment