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Prostaglandin E(2) Reverses Curcumin-Induced Inhibition of Survival Signal Pathways in Human Colorectal Carcinoma (HCT-15) Cell Lines

Prostaglandin E(2) (PGE(2)) promotes tumor-persistent inflammation, frequently resulting in cancer. Curcumin is a diphenolic turmeric that inhibits carcinogenesis and induces apoptosis. PGE(2) inhibits curcumin-induced apoptosis; however, the underlying inhibitory mechanisms in colon cancer cells re...

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Autores principales: Shehzad, Adeeb, Islam, Salman Ul, Lee, Jaetae, Lee, Young Sup
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Molecular and Cellular Biology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4275707/
https://www.ncbi.nlm.nih.gov/pubmed/25431425
http://dx.doi.org/10.14348/molcells.2014.0212
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author Shehzad, Adeeb
Islam, Salman Ul
Lee, Jaetae
Lee, Young Sup
author_facet Shehzad, Adeeb
Islam, Salman Ul
Lee, Jaetae
Lee, Young Sup
author_sort Shehzad, Adeeb
collection PubMed
description Prostaglandin E(2) (PGE(2)) promotes tumor-persistent inflammation, frequently resulting in cancer. Curcumin is a diphenolic turmeric that inhibits carcinogenesis and induces apoptosis. PGE(2) inhibits curcumin-induced apoptosis; however, the underlying inhibitory mechanisms in colon cancer cells remain unknown. The aim of the present study is to investigate the survival role of PGE(2) and whether addition of exogenous PGE(2) affects curcumin-induced cell death. HCT-15 cells were treated with curcumin and PGE(2), and protein expression levels were investigated via Western blot. Reactive oxygen species (ROS) generation, lipid peroxidation, and intracellular glutathione (GSH) levels were confirmed using specific dyes. The nuclear factor-kappa B (NF-κB) DNA-binding was measured by electrophoretic mobility shift assay (EMSA). PGE(2) inhibited curcumin-induced apoptosis by suppressing oxidative stress and degradation of PARP and lamin B. However, exposure of cells to the EP2 receptor antagonist, AH6809, and the PKA inhibitor, H89, before treatment with PGE(2) or curcumin abolished the protective effect of PGE(2) and enhanced curcumin-induced cell death. PGE(2) activates PKA, which is required for cAMP-mediated transcriptional activation of CREB. PGE(2) also activated the Ras/Raf/Erk pathway, and pretreatment with PD98059 abolished the protective effect of PGE(2). Furthermore, curcumin treatment greatly reduced phosphorylation of CREB, followed by a concomitant reduction of NF-κB (p50 and p65) subunit activation. PGE(2) markedly activated nuclear translocation of NF-κB. EMSA confirmed the DNA-binding activities of NF-κB subunits. These results suggest that inhibition of curcumin-induced apoptosis by PGE(2) through activation of PKA, Ras, and NF-κB signaling pathways may provide a molecular basis for the reversal of curcumin-induced colon carcinoma cell death.
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spelling pubmed-42757072014-12-24 Prostaglandin E(2) Reverses Curcumin-Induced Inhibition of Survival Signal Pathways in Human Colorectal Carcinoma (HCT-15) Cell Lines Shehzad, Adeeb Islam, Salman Ul Lee, Jaetae Lee, Young Sup Mol Cells Article Prostaglandin E(2) (PGE(2)) promotes tumor-persistent inflammation, frequently resulting in cancer. Curcumin is a diphenolic turmeric that inhibits carcinogenesis and induces apoptosis. PGE(2) inhibits curcumin-induced apoptosis; however, the underlying inhibitory mechanisms in colon cancer cells remain unknown. The aim of the present study is to investigate the survival role of PGE(2) and whether addition of exogenous PGE(2) affects curcumin-induced cell death. HCT-15 cells were treated with curcumin and PGE(2), and protein expression levels were investigated via Western blot. Reactive oxygen species (ROS) generation, lipid peroxidation, and intracellular glutathione (GSH) levels were confirmed using specific dyes. The nuclear factor-kappa B (NF-κB) DNA-binding was measured by electrophoretic mobility shift assay (EMSA). PGE(2) inhibited curcumin-induced apoptosis by suppressing oxidative stress and degradation of PARP and lamin B. However, exposure of cells to the EP2 receptor antagonist, AH6809, and the PKA inhibitor, H89, before treatment with PGE(2) or curcumin abolished the protective effect of PGE(2) and enhanced curcumin-induced cell death. PGE(2) activates PKA, which is required for cAMP-mediated transcriptional activation of CREB. PGE(2) also activated the Ras/Raf/Erk pathway, and pretreatment with PD98059 abolished the protective effect of PGE(2). Furthermore, curcumin treatment greatly reduced phosphorylation of CREB, followed by a concomitant reduction of NF-κB (p50 and p65) subunit activation. PGE(2) markedly activated nuclear translocation of NF-κB. EMSA confirmed the DNA-binding activities of NF-κB subunits. These results suggest that inhibition of curcumin-induced apoptosis by PGE(2) through activation of PKA, Ras, and NF-κB signaling pathways may provide a molecular basis for the reversal of curcumin-induced colon carcinoma cell death. Korean Society for Molecular and Cellular Biology 2014-12-31 2014-11-26 /pmc/articles/PMC4275707/ /pubmed/25431425 http://dx.doi.org/10.14348/molcells.2014.0212 Text en © The Korean Society for Molecular and Cellular Biology. All rights reserved. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
spellingShingle Article
Shehzad, Adeeb
Islam, Salman Ul
Lee, Jaetae
Lee, Young Sup
Prostaglandin E(2) Reverses Curcumin-Induced Inhibition of Survival Signal Pathways in Human Colorectal Carcinoma (HCT-15) Cell Lines
title Prostaglandin E(2) Reverses Curcumin-Induced Inhibition of Survival Signal Pathways in Human Colorectal Carcinoma (HCT-15) Cell Lines
title_full Prostaglandin E(2) Reverses Curcumin-Induced Inhibition of Survival Signal Pathways in Human Colorectal Carcinoma (HCT-15) Cell Lines
title_fullStr Prostaglandin E(2) Reverses Curcumin-Induced Inhibition of Survival Signal Pathways in Human Colorectal Carcinoma (HCT-15) Cell Lines
title_full_unstemmed Prostaglandin E(2) Reverses Curcumin-Induced Inhibition of Survival Signal Pathways in Human Colorectal Carcinoma (HCT-15) Cell Lines
title_short Prostaglandin E(2) Reverses Curcumin-Induced Inhibition of Survival Signal Pathways in Human Colorectal Carcinoma (HCT-15) Cell Lines
title_sort prostaglandin e(2) reverses curcumin-induced inhibition of survival signal pathways in human colorectal carcinoma (hct-15) cell lines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4275707/
https://www.ncbi.nlm.nih.gov/pubmed/25431425
http://dx.doi.org/10.14348/molcells.2014.0212
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