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Sesamol: A Natural Phenolic Compound with Promising Anticandidal Potential

We investigated the antifungal effects of sesamol (Ses), a natural phenolic compound, and exemplified that it could be mediated through disruption of calcineurin signaling pathway in C. albicans, a human fungal pathogen. The repertoire of antifungal activity not only was limited to C. albicans and i...

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Detalles Bibliográficos
Autores principales: Ansari, Moiz A., Fatima, Zeeshan, Hameed, Saif
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4276329/
https://www.ncbi.nlm.nih.gov/pubmed/25574401
http://dx.doi.org/10.1155/2014/895193
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author Ansari, Moiz A.
Fatima, Zeeshan
Hameed, Saif
author_facet Ansari, Moiz A.
Fatima, Zeeshan
Hameed, Saif
author_sort Ansari, Moiz A.
collection PubMed
description We investigated the antifungal effects of sesamol (Ses), a natural phenolic compound, and exemplified that it could be mediated through disruption of calcineurin signaling pathway in C. albicans, a human fungal pathogen. The repertoire of antifungal activity not only was limited to C. albicans and its six clinical isolates tested but also was against non-albicans species of Candida. Interestingly, the antifungal effect of Ses affects neither the MDR efflux transporter activity nor passive diffusion of drug. We found that C. albicans treated with Ses copies the phenotype displayed by cells having defect in calcineurin signaling leading to sensitivity against alkaline pH, ionic, membrane, salinity, endoplasmic reticulum, and serum stresses but remained resistant to thermal stress. Furthermore, the ergosterol levels were significantly decreased by 63% confirming membrane perturbations in response to Ses as also visualized through transmission electron micrographs. Despite the fact that Ses treatment mimics the phenotype of compromised calcineurin signaling, it was independent of cell wall integrity pathway as revealed by spot assays and the scanning electron micrographs. Taken together, the data procured from this study clearly ascertains that Ses is an effectual antifungal agent that could be competently employed in treating Candida infections.
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spelling pubmed-42763292015-01-08 Sesamol: A Natural Phenolic Compound with Promising Anticandidal Potential Ansari, Moiz A. Fatima, Zeeshan Hameed, Saif J Pathog Research Article We investigated the antifungal effects of sesamol (Ses), a natural phenolic compound, and exemplified that it could be mediated through disruption of calcineurin signaling pathway in C. albicans, a human fungal pathogen. The repertoire of antifungal activity not only was limited to C. albicans and its six clinical isolates tested but also was against non-albicans species of Candida. Interestingly, the antifungal effect of Ses affects neither the MDR efflux transporter activity nor passive diffusion of drug. We found that C. albicans treated with Ses copies the phenotype displayed by cells having defect in calcineurin signaling leading to sensitivity against alkaline pH, ionic, membrane, salinity, endoplasmic reticulum, and serum stresses but remained resistant to thermal stress. Furthermore, the ergosterol levels were significantly decreased by 63% confirming membrane perturbations in response to Ses as also visualized through transmission electron micrographs. Despite the fact that Ses treatment mimics the phenotype of compromised calcineurin signaling, it was independent of cell wall integrity pathway as revealed by spot assays and the scanning electron micrographs. Taken together, the data procured from this study clearly ascertains that Ses is an effectual antifungal agent that could be competently employed in treating Candida infections. Hindawi Publishing Corporation 2014 2014-12-09 /pmc/articles/PMC4276329/ /pubmed/25574401 http://dx.doi.org/10.1155/2014/895193 Text en Copyright © 2014 Moiz A. Ansari et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ansari, Moiz A.
Fatima, Zeeshan
Hameed, Saif
Sesamol: A Natural Phenolic Compound with Promising Anticandidal Potential
title Sesamol: A Natural Phenolic Compound with Promising Anticandidal Potential
title_full Sesamol: A Natural Phenolic Compound with Promising Anticandidal Potential
title_fullStr Sesamol: A Natural Phenolic Compound with Promising Anticandidal Potential
title_full_unstemmed Sesamol: A Natural Phenolic Compound with Promising Anticandidal Potential
title_short Sesamol: A Natural Phenolic Compound with Promising Anticandidal Potential
title_sort sesamol: a natural phenolic compound with promising anticandidal potential
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4276329/
https://www.ncbi.nlm.nih.gov/pubmed/25574401
http://dx.doi.org/10.1155/2014/895193
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