Detection of Influenza Virus Infection Using Two PCR Methods

Rapid, accurate, and cost-effective methods to identify the cause of respiratory tract infections are needed to maximize clinical benefit. Outpatients with acute respiratory illness were tested for influenza using a singleplex reverse transcriptase polymerase chain reaction (SRT-PCR) method. A multi...

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Autores principales: Zimmerman, Richard K., Rinaldo, Charles R., Nowalk, Mary Patricia, Balasubramani, G. K., Thompson, Mark G., Bullotta, Arlene, Susick, Michael, Wisniewski, Stephen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4276355/
https://www.ncbi.nlm.nih.gov/pubmed/25574169
http://dx.doi.org/10.1155/2014/274679
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author Zimmerman, Richard K.
Rinaldo, Charles R.
Nowalk, Mary Patricia
Balasubramani, G. K.
Thompson, Mark G.
Bullotta, Arlene
Susick, Michael
Wisniewski, Stephen
author_facet Zimmerman, Richard K.
Rinaldo, Charles R.
Nowalk, Mary Patricia
Balasubramani, G. K.
Thompson, Mark G.
Bullotta, Arlene
Susick, Michael
Wisniewski, Stephen
author_sort Zimmerman, Richard K.
collection PubMed
description Rapid, accurate, and cost-effective methods to identify the cause of respiratory tract infections are needed to maximize clinical benefit. Outpatients with acute respiratory illness were tested for influenza using a singleplex reverse transcriptase polymerase chain reaction (SRT-PCR) method. A multiplex RT-PCR (MRT-PCR) method tested for influenza and 17 other viruses and was compared with SRT-PCR using chi-square tests. Among 935 patients, 335 (36%) tested positive for influenza A and influenza B using SRT-PCR. Using MRT-PCR, 320 (34.2%) tested positive for influenza A and influenza B. This study supports MRT-PCR as a comparable method for detecting influenza among patients seeking outpatient care for acute respiratory illnesses.
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spelling pubmed-42763552015-01-08 Detection of Influenza Virus Infection Using Two PCR Methods Zimmerman, Richard K. Rinaldo, Charles R. Nowalk, Mary Patricia Balasubramani, G. K. Thompson, Mark G. Bullotta, Arlene Susick, Michael Wisniewski, Stephen Adv Virol Research Article Rapid, accurate, and cost-effective methods to identify the cause of respiratory tract infections are needed to maximize clinical benefit. Outpatients with acute respiratory illness were tested for influenza using a singleplex reverse transcriptase polymerase chain reaction (SRT-PCR) method. A multiplex RT-PCR (MRT-PCR) method tested for influenza and 17 other viruses and was compared with SRT-PCR using chi-square tests. Among 935 patients, 335 (36%) tested positive for influenza A and influenza B using SRT-PCR. Using MRT-PCR, 320 (34.2%) tested positive for influenza A and influenza B. This study supports MRT-PCR as a comparable method for detecting influenza among patients seeking outpatient care for acute respiratory illnesses. Hindawi Publishing Corporation 2014 2014-12-09 /pmc/articles/PMC4276355/ /pubmed/25574169 http://dx.doi.org/10.1155/2014/274679 Text en Copyright © 2014 Richard K. Zimmerman et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zimmerman, Richard K.
Rinaldo, Charles R.
Nowalk, Mary Patricia
Balasubramani, G. K.
Thompson, Mark G.
Bullotta, Arlene
Susick, Michael
Wisniewski, Stephen
Detection of Influenza Virus Infection Using Two PCR Methods
title Detection of Influenza Virus Infection Using Two PCR Methods
title_full Detection of Influenza Virus Infection Using Two PCR Methods
title_fullStr Detection of Influenza Virus Infection Using Two PCR Methods
title_full_unstemmed Detection of Influenza Virus Infection Using Two PCR Methods
title_short Detection of Influenza Virus Infection Using Two PCR Methods
title_sort detection of influenza virus infection using two pcr methods
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4276355/
https://www.ncbi.nlm.nih.gov/pubmed/25574169
http://dx.doi.org/10.1155/2014/274679
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