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A post-mortem stereological study of striatal cell number in human obesity

OBJECTIVE: Neuroimaging studies have revealed abnormalities in brain structure, including the striatum, in obese people. We aimed to investigate the cellular and parenchymal basis for these findings in post-mortem brain tissue. DESIGN AND METHODS: Design-based (unbiased) stereology combined with his...

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Autores principales: Weise, Christopher M., Mouton, Peter R, Eschbacher, Jennifer, Coons, Stephen W., Krakoff, Jonathan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4276484/
https://www.ncbi.nlm.nih.gov/pubmed/25234737
http://dx.doi.org/10.1002/oby.20897
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author Weise, Christopher M.
Mouton, Peter R
Eschbacher, Jennifer
Coons, Stephen W.
Krakoff, Jonathan
author_facet Weise, Christopher M.
Mouton, Peter R
Eschbacher, Jennifer
Coons, Stephen W.
Krakoff, Jonathan
author_sort Weise, Christopher M.
collection PubMed
description OBJECTIVE: Neuroimaging studies have revealed abnormalities in brain structure, including the striatum, in obese people. We aimed to investigate the cellular and parenchymal basis for these findings in post-mortem brain tissue. DESIGN AND METHODS: Design-based (unbiased) stereology combined with histochemical and immunocytochemical staining were used to quantify total number of neurons and astrocytes in post-mortem striatal brain samples from 9 obese (BMI 40.2±6.1 kg*m(−2)) and 8 lean (BMI 24.4±1.0 kg*m(−2)) donors. Total numbers of Nissl-stained neurons and GFAP-immunopositive astrocytes were counted in ten systematic-random sections starting from the frontal pole of the striatum. RESULTS: There were no differences in mean total numbers of neurons (obese: 7.60E+06; SD 2.50E+06; lean: 7.85E+06; SD 8.26E+05; p<0.78) or astrocytes (obese: 7.42E+06; SD 2.27E+06; lean: 7.43E+06; SD 2.50E+06; p<0.99). A higher variance was found for number of neurons (p<0.007) but not astrocytes (p<0.72) in the obese group. Neuron/glia ratios were similar in both groups (obese: 1.07; SD 0.39; lean: 1.15; SD 0.37; p<0.70) with an overall striatal neuron/glia ratio of 1.11 (SD 0.37) across the entire study population (n=17). CONCLUSION: We found no difference in the average numbers of neurons and astrocytes in the anterior striatum between lean and obese people. The morphological basis for structural brain changes in obesity requires further investigation.
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spelling pubmed-42764842016-01-01 A post-mortem stereological study of striatal cell number in human obesity Weise, Christopher M. Mouton, Peter R Eschbacher, Jennifer Coons, Stephen W. Krakoff, Jonathan Obesity (Silver Spring) Article OBJECTIVE: Neuroimaging studies have revealed abnormalities in brain structure, including the striatum, in obese people. We aimed to investigate the cellular and parenchymal basis for these findings in post-mortem brain tissue. DESIGN AND METHODS: Design-based (unbiased) stereology combined with histochemical and immunocytochemical staining were used to quantify total number of neurons and astrocytes in post-mortem striatal brain samples from 9 obese (BMI 40.2±6.1 kg*m(−2)) and 8 lean (BMI 24.4±1.0 kg*m(−2)) donors. Total numbers of Nissl-stained neurons and GFAP-immunopositive astrocytes were counted in ten systematic-random sections starting from the frontal pole of the striatum. RESULTS: There were no differences in mean total numbers of neurons (obese: 7.60E+06; SD 2.50E+06; lean: 7.85E+06; SD 8.26E+05; p<0.78) or astrocytes (obese: 7.42E+06; SD 2.27E+06; lean: 7.43E+06; SD 2.50E+06; p<0.99). A higher variance was found for number of neurons (p<0.007) but not astrocytes (p<0.72) in the obese group. Neuron/glia ratios were similar in both groups (obese: 1.07; SD 0.39; lean: 1.15; SD 0.37; p<0.70) with an overall striatal neuron/glia ratio of 1.11 (SD 0.37) across the entire study population (n=17). CONCLUSION: We found no difference in the average numbers of neurons and astrocytes in the anterior striatum between lean and obese people. The morphological basis for structural brain changes in obesity requires further investigation. 2014-09-19 2015-01 /pmc/articles/PMC4276484/ /pubmed/25234737 http://dx.doi.org/10.1002/oby.20897 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Weise, Christopher M.
Mouton, Peter R
Eschbacher, Jennifer
Coons, Stephen W.
Krakoff, Jonathan
A post-mortem stereological study of striatal cell number in human obesity
title A post-mortem stereological study of striatal cell number in human obesity
title_full A post-mortem stereological study of striatal cell number in human obesity
title_fullStr A post-mortem stereological study of striatal cell number in human obesity
title_full_unstemmed A post-mortem stereological study of striatal cell number in human obesity
title_short A post-mortem stereological study of striatal cell number in human obesity
title_sort post-mortem stereological study of striatal cell number in human obesity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4276484/
https://www.ncbi.nlm.nih.gov/pubmed/25234737
http://dx.doi.org/10.1002/oby.20897
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