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A post-mortem stereological study of striatal cell number in human obesity
OBJECTIVE: Neuroimaging studies have revealed abnormalities in brain structure, including the striatum, in obese people. We aimed to investigate the cellular and parenchymal basis for these findings in post-mortem brain tissue. DESIGN AND METHODS: Design-based (unbiased) stereology combined with his...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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2014
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4276484/ https://www.ncbi.nlm.nih.gov/pubmed/25234737 http://dx.doi.org/10.1002/oby.20897 |
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author | Weise, Christopher M. Mouton, Peter R Eschbacher, Jennifer Coons, Stephen W. Krakoff, Jonathan |
author_facet | Weise, Christopher M. Mouton, Peter R Eschbacher, Jennifer Coons, Stephen W. Krakoff, Jonathan |
author_sort | Weise, Christopher M. |
collection | PubMed |
description | OBJECTIVE: Neuroimaging studies have revealed abnormalities in brain structure, including the striatum, in obese people. We aimed to investigate the cellular and parenchymal basis for these findings in post-mortem brain tissue. DESIGN AND METHODS: Design-based (unbiased) stereology combined with histochemical and immunocytochemical staining were used to quantify total number of neurons and astrocytes in post-mortem striatal brain samples from 9 obese (BMI 40.2±6.1 kg*m(−2)) and 8 lean (BMI 24.4±1.0 kg*m(−2)) donors. Total numbers of Nissl-stained neurons and GFAP-immunopositive astrocytes were counted in ten systematic-random sections starting from the frontal pole of the striatum. RESULTS: There were no differences in mean total numbers of neurons (obese: 7.60E+06; SD 2.50E+06; lean: 7.85E+06; SD 8.26E+05; p<0.78) or astrocytes (obese: 7.42E+06; SD 2.27E+06; lean: 7.43E+06; SD 2.50E+06; p<0.99). A higher variance was found for number of neurons (p<0.007) but not astrocytes (p<0.72) in the obese group. Neuron/glia ratios were similar in both groups (obese: 1.07; SD 0.39; lean: 1.15; SD 0.37; p<0.70) with an overall striatal neuron/glia ratio of 1.11 (SD 0.37) across the entire study population (n=17). CONCLUSION: We found no difference in the average numbers of neurons and astrocytes in the anterior striatum between lean and obese people. The morphological basis for structural brain changes in obesity requires further investigation. |
format | Online Article Text |
id | pubmed-4276484 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
record_format | MEDLINE/PubMed |
spelling | pubmed-42764842016-01-01 A post-mortem stereological study of striatal cell number in human obesity Weise, Christopher M. Mouton, Peter R Eschbacher, Jennifer Coons, Stephen W. Krakoff, Jonathan Obesity (Silver Spring) Article OBJECTIVE: Neuroimaging studies have revealed abnormalities in brain structure, including the striatum, in obese people. We aimed to investigate the cellular and parenchymal basis for these findings in post-mortem brain tissue. DESIGN AND METHODS: Design-based (unbiased) stereology combined with histochemical and immunocytochemical staining were used to quantify total number of neurons and astrocytes in post-mortem striatal brain samples from 9 obese (BMI 40.2±6.1 kg*m(−2)) and 8 lean (BMI 24.4±1.0 kg*m(−2)) donors. Total numbers of Nissl-stained neurons and GFAP-immunopositive astrocytes were counted in ten systematic-random sections starting from the frontal pole of the striatum. RESULTS: There were no differences in mean total numbers of neurons (obese: 7.60E+06; SD 2.50E+06; lean: 7.85E+06; SD 8.26E+05; p<0.78) or astrocytes (obese: 7.42E+06; SD 2.27E+06; lean: 7.43E+06; SD 2.50E+06; p<0.99). A higher variance was found for number of neurons (p<0.007) but not astrocytes (p<0.72) in the obese group. Neuron/glia ratios were similar in both groups (obese: 1.07; SD 0.39; lean: 1.15; SD 0.37; p<0.70) with an overall striatal neuron/glia ratio of 1.11 (SD 0.37) across the entire study population (n=17). CONCLUSION: We found no difference in the average numbers of neurons and astrocytes in the anterior striatum between lean and obese people. The morphological basis for structural brain changes in obesity requires further investigation. 2014-09-19 2015-01 /pmc/articles/PMC4276484/ /pubmed/25234737 http://dx.doi.org/10.1002/oby.20897 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Weise, Christopher M. Mouton, Peter R Eschbacher, Jennifer Coons, Stephen W. Krakoff, Jonathan A post-mortem stereological study of striatal cell number in human obesity |
title | A post-mortem stereological study of striatal cell number in human obesity |
title_full | A post-mortem stereological study of striatal cell number in human obesity |
title_fullStr | A post-mortem stereological study of striatal cell number in human obesity |
title_full_unstemmed | A post-mortem stereological study of striatal cell number in human obesity |
title_short | A post-mortem stereological study of striatal cell number in human obesity |
title_sort | post-mortem stereological study of striatal cell number in human obesity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4276484/ https://www.ncbi.nlm.nih.gov/pubmed/25234737 http://dx.doi.org/10.1002/oby.20897 |
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