Influenza pH1N1 Virus Accumulated H275Y Mutation in Neuraminidase during Propagation in MDCK Cells

Here, we sequenced the genome of the influenza A/Finland/741 M/2014(H1N1) virus and found that the virus accumulated oseltamivir resistance mutation H275Y in its neuraminidase during propagation in cell culture. This indicates that propagation in cell culture modifies virus genomes. The instability...

Descripción completa

Detalles Bibliográficos
Autores principales: Mishel, Polina, Bychkov, Dmitrii, Kallio-Kokko, Hannimari, Valkonen, Miia, Kantele, Anu, Mattila, Pirkko, Almusa, Henrikki, Jalovaara, Petri, Kainov, Denis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2014
Materias:
Viruses
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4276832/
https://www.ncbi.nlm.nih.gov/pubmed/25540354
http://dx.doi.org/10.1128/genomeA.01349-14
_version_ 1782350307039117312
author Mishel, Polina
Bychkov, Dmitrii
Kallio-Kokko, Hannimari
Valkonen, Miia
Kantele, Anu
Mattila, Pirkko
Almusa, Henrikki
Jalovaara, Petri
Kainov, Denis
author_facet Mishel, Polina
Bychkov, Dmitrii
Kallio-Kokko, Hannimari
Valkonen, Miia
Kantele, Anu
Mattila, Pirkko
Almusa, Henrikki
Jalovaara, Petri
Kainov, Denis
author_sort Mishel, Polina
collection PubMed
description Here, we sequenced the genome of the influenza A/Finland/741 M/2014(H1N1) virus and found that the virus accumulated oseltamivir resistance mutation H275Y in its neuraminidase during propagation in cell culture. This indicates that propagation in cell culture modifies virus genomes. The instability of influenza genomes should be taken into consideration during drug-sensitivity studies.
format Online
Article
Text
id pubmed-4276832
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher American Society for Microbiology
record_format MEDLINE/PubMed
spelling pubmed-42768322014-12-31 Influenza pH1N1 Virus Accumulated H275Y Mutation in Neuraminidase during Propagation in MDCK Cells Mishel, Polina Bychkov, Dmitrii Kallio-Kokko, Hannimari Valkonen, Miia Kantele, Anu Mattila, Pirkko Almusa, Henrikki Jalovaara, Petri Kainov, Denis Genome Announc Viruses Here, we sequenced the genome of the influenza A/Finland/741 M/2014(H1N1) virus and found that the virus accumulated oseltamivir resistance mutation H275Y in its neuraminidase during propagation in cell culture. This indicates that propagation in cell culture modifies virus genomes. The instability of influenza genomes should be taken into consideration during drug-sensitivity studies. American Society for Microbiology 2014-12-24 /pmc/articles/PMC4276832/ /pubmed/25540354 http://dx.doi.org/10.1128/genomeA.01349-14 Text en Copyright © 2014 Mishel et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license (http://creativecommons.org/licenses/by/3.0/) .
spellingShingle Viruses
Mishel, Polina
Bychkov, Dmitrii
Kallio-Kokko, Hannimari
Valkonen, Miia
Kantele, Anu
Mattila, Pirkko
Almusa, Henrikki
Jalovaara, Petri
Kainov, Denis
Influenza pH1N1 Virus Accumulated H275Y Mutation in Neuraminidase during Propagation in MDCK Cells
title Influenza pH1N1 Virus Accumulated H275Y Mutation in Neuraminidase during Propagation in MDCK Cells
title_full Influenza pH1N1 Virus Accumulated H275Y Mutation in Neuraminidase during Propagation in MDCK Cells
title_fullStr Influenza pH1N1 Virus Accumulated H275Y Mutation in Neuraminidase during Propagation in MDCK Cells
title_full_unstemmed Influenza pH1N1 Virus Accumulated H275Y Mutation in Neuraminidase during Propagation in MDCK Cells
title_short Influenza pH1N1 Virus Accumulated H275Y Mutation in Neuraminidase during Propagation in MDCK Cells
title_sort influenza ph1n1 virus accumulated h275y mutation in neuraminidase during propagation in mdck cells
topic Viruses
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4276832/
https://www.ncbi.nlm.nih.gov/pubmed/25540354
http://dx.doi.org/10.1128/genomeA.01349-14
work_keys_str_mv AT mishelpolina influenzaph1n1virusaccumulatedh275ymutationinneuraminidaseduringpropagationinmdckcells
AT bychkovdmitrii influenzaph1n1virusaccumulatedh275ymutationinneuraminidaseduringpropagationinmdckcells
AT kalliokokkohannimari influenzaph1n1virusaccumulatedh275ymutationinneuraminidaseduringpropagationinmdckcells
AT valkonenmiia influenzaph1n1virusaccumulatedh275ymutationinneuraminidaseduringpropagationinmdckcells
AT kanteleanu influenzaph1n1virusaccumulatedh275ymutationinneuraminidaseduringpropagationinmdckcells
AT mattilapirkko influenzaph1n1virusaccumulatedh275ymutationinneuraminidaseduringpropagationinmdckcells
AT almusahenrikki influenzaph1n1virusaccumulatedh275ymutationinneuraminidaseduringpropagationinmdckcells
AT jalovaarapetri influenzaph1n1virusaccumulatedh275ymutationinneuraminidaseduringpropagationinmdckcells
AT kainovdenis influenzaph1n1virusaccumulatedh275ymutationinneuraminidaseduringpropagationinmdckcells

Ejemplares similares