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Neurochemical Effects of Chronic Administration of Calcitriol in Rats

Despite accumulating data showing the various neurological actions of vitamin D (VD), its effects on brain neurochemistry are still far from fully understood. To further investigate the neurochemical influence of VD, we assessed neurotransmitter systems in the brain of rats following 6-week calcitri...

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Autores principales: Jiang, Pei, Zhang, Li-Hong, Cai, Hua-Lin, Li, Huan-De, Liu, Yi-Ping, Tang, Mi-Mi, Dang, Rui-Li, Zhu, Wen-Ye, Xue, Ying, He, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4277014/
https://www.ncbi.nlm.nih.gov/pubmed/25533012
http://dx.doi.org/10.3390/nu6126048
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author Jiang, Pei
Zhang, Li-Hong
Cai, Hua-Lin
Li, Huan-De
Liu, Yi-Ping
Tang, Mi-Mi
Dang, Rui-Li
Zhu, Wen-Ye
Xue, Ying
He, Xin
author_facet Jiang, Pei
Zhang, Li-Hong
Cai, Hua-Lin
Li, Huan-De
Liu, Yi-Ping
Tang, Mi-Mi
Dang, Rui-Li
Zhu, Wen-Ye
Xue, Ying
He, Xin
author_sort Jiang, Pei
collection PubMed
description Despite accumulating data showing the various neurological actions of vitamin D (VD), its effects on brain neurochemistry are still far from fully understood. To further investigate the neurochemical influence of VD, we assessed neurotransmitter systems in the brain of rats following 6-week calcitriol (1,25-dihydroxyvitamin D) administration (50 ng/kg/day or 100 ng/kg/day). Both the two doses of calcitriol enhanced VDR protein level without affecting serum calcium and phosphate status. Rats treated with calcitriol, especially with the higher dose, exhibited elevated γ-aminobutyric acid (GABA) status. Correspondingly, the mRNA expression of glutamate decarboxylase (GAD) 67 was increased. 100 ng/kg of calcitriol administration also increased glutamate and glutamine levels in the prefrontal cortex, but did not alter glutamine synthetase (GS) expression. Additionally, calcitriol treatment promoted tyrosine hydroxylase (TH) and tryptophan hydroxylase 2 (TPH2) expression without changing dopamine and serotonin status. However, the concentrations of the metabolites of dopamine and serotonin were increased and the drug use also resulted in a significant rise of monoamine oxidase A (MAO(A)) expression, which might be responsible to maintain the homeostasis of dopaminergic and serotonergic neurotransmission. Collectively, the present study firstly showed the effects of calcitriol in the major neurotransmitter systems, providing new evidence for the role of VD in brain function.
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spelling pubmed-42770142015-01-15 Neurochemical Effects of Chronic Administration of Calcitriol in Rats Jiang, Pei Zhang, Li-Hong Cai, Hua-Lin Li, Huan-De Liu, Yi-Ping Tang, Mi-Mi Dang, Rui-Li Zhu, Wen-Ye Xue, Ying He, Xin Nutrients Article Despite accumulating data showing the various neurological actions of vitamin D (VD), its effects on brain neurochemistry are still far from fully understood. To further investigate the neurochemical influence of VD, we assessed neurotransmitter systems in the brain of rats following 6-week calcitriol (1,25-dihydroxyvitamin D) administration (50 ng/kg/day or 100 ng/kg/day). Both the two doses of calcitriol enhanced VDR protein level without affecting serum calcium and phosphate status. Rats treated with calcitriol, especially with the higher dose, exhibited elevated γ-aminobutyric acid (GABA) status. Correspondingly, the mRNA expression of glutamate decarboxylase (GAD) 67 was increased. 100 ng/kg of calcitriol administration also increased glutamate and glutamine levels in the prefrontal cortex, but did not alter glutamine synthetase (GS) expression. Additionally, calcitriol treatment promoted tyrosine hydroxylase (TH) and tryptophan hydroxylase 2 (TPH2) expression without changing dopamine and serotonin status. However, the concentrations of the metabolites of dopamine and serotonin were increased and the drug use also resulted in a significant rise of monoamine oxidase A (MAO(A)) expression, which might be responsible to maintain the homeostasis of dopaminergic and serotonergic neurotransmission. Collectively, the present study firstly showed the effects of calcitriol in the major neurotransmitter systems, providing new evidence for the role of VD in brain function. MDPI 2014-12-22 /pmc/articles/PMC4277014/ /pubmed/25533012 http://dx.doi.org/10.3390/nu6126048 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jiang, Pei
Zhang, Li-Hong
Cai, Hua-Lin
Li, Huan-De
Liu, Yi-Ping
Tang, Mi-Mi
Dang, Rui-Li
Zhu, Wen-Ye
Xue, Ying
He, Xin
Neurochemical Effects of Chronic Administration of Calcitriol in Rats
title Neurochemical Effects of Chronic Administration of Calcitriol in Rats
title_full Neurochemical Effects of Chronic Administration of Calcitriol in Rats
title_fullStr Neurochemical Effects of Chronic Administration of Calcitriol in Rats
title_full_unstemmed Neurochemical Effects of Chronic Administration of Calcitriol in Rats
title_short Neurochemical Effects of Chronic Administration of Calcitriol in Rats
title_sort neurochemical effects of chronic administration of calcitriol in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4277014/
https://www.ncbi.nlm.nih.gov/pubmed/25533012
http://dx.doi.org/10.3390/nu6126048
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