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Trichomonas vaginalis Metalloproteinase Induces mTOR Cleavage of SiHa Cells
Trichomonas vaginalis secretes a number of proteases which are suspected to be the cause of pathogenesis; however, little is understood how they manipulate host cells. The mammalian target of rapamycin (mTOR) regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis,...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society for Parasitology and Tropical Medicine
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4277021/ https://www.ncbi.nlm.nih.gov/pubmed/25548410 http://dx.doi.org/10.3347/kjp.2014.52.6.595 |
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author | Quan, Juan-Hua Choi, In-Wook Yang, Jung-Bo Zhou, Wei Cha, Guang-Ho Zhou, Yu Ryu, Jae-Sook Lee, Young-Ha |
author_facet | Quan, Juan-Hua Choi, In-Wook Yang, Jung-Bo Zhou, Wei Cha, Guang-Ho Zhou, Yu Ryu, Jae-Sook Lee, Young-Ha |
author_sort | Quan, Juan-Hua |
collection | PubMed |
description | Trichomonas vaginalis secretes a number of proteases which are suspected to be the cause of pathogenesis; however, little is understood how they manipulate host cells. The mammalian target of rapamycin (mTOR) regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis, and transcription. We detected various types of metalloproteinases including GP63 protein from T. vaginalis trophozoites, and T. vaginalis GP63 metalloproteinase was confirmed by sequencing and western blot. When SiHa cells were stimulated with live T. vaginalis, T. vaginalis excretory-secretory products (ESP) or T. vaginalis lysate, live T. vaginalis and T. vaginalis ESP induced the mTOR cleavage in both time- and parasite load-dependent manner, but T. vaginalis lysate did not. Pretreatment of T. vaginalis with a metalloproteinase inhibitor, 1,10-phenanthroline, completely disappeared the mTOR cleavage in SiHa cells. Collectively, T. vaginalis metallopeptidase induces host cell mTOR cleavage, which may be related to survival of the parasite. |
format | Online Article Text |
id | pubmed-4277021 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | The Korean Society for Parasitology and Tropical Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-42770212014-12-29 Trichomonas vaginalis Metalloproteinase Induces mTOR Cleavage of SiHa Cells Quan, Juan-Hua Choi, In-Wook Yang, Jung-Bo Zhou, Wei Cha, Guang-Ho Zhou, Yu Ryu, Jae-Sook Lee, Young-Ha Korean J Parasitol Original Article Trichomonas vaginalis secretes a number of proteases which are suspected to be the cause of pathogenesis; however, little is understood how they manipulate host cells. The mammalian target of rapamycin (mTOR) regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis, and transcription. We detected various types of metalloproteinases including GP63 protein from T. vaginalis trophozoites, and T. vaginalis GP63 metalloproteinase was confirmed by sequencing and western blot. When SiHa cells were stimulated with live T. vaginalis, T. vaginalis excretory-secretory products (ESP) or T. vaginalis lysate, live T. vaginalis and T. vaginalis ESP induced the mTOR cleavage in both time- and parasite load-dependent manner, but T. vaginalis lysate did not. Pretreatment of T. vaginalis with a metalloproteinase inhibitor, 1,10-phenanthroline, completely disappeared the mTOR cleavage in SiHa cells. Collectively, T. vaginalis metallopeptidase induces host cell mTOR cleavage, which may be related to survival of the parasite. The Korean Society for Parasitology and Tropical Medicine 2014-12 2014-12-23 /pmc/articles/PMC4277021/ /pubmed/25548410 http://dx.doi.org/10.3347/kjp.2014.52.6.595 Text en © 2014, Korean Society for Parasitology and Tropical Medicine http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Quan, Juan-Hua Choi, In-Wook Yang, Jung-Bo Zhou, Wei Cha, Guang-Ho Zhou, Yu Ryu, Jae-Sook Lee, Young-Ha Trichomonas vaginalis Metalloproteinase Induces mTOR Cleavage of SiHa Cells |
title | Trichomonas vaginalis Metalloproteinase Induces mTOR Cleavage of SiHa Cells |
title_full | Trichomonas vaginalis Metalloproteinase Induces mTOR Cleavage of SiHa Cells |
title_fullStr | Trichomonas vaginalis Metalloproteinase Induces mTOR Cleavage of SiHa Cells |
title_full_unstemmed | Trichomonas vaginalis Metalloproteinase Induces mTOR Cleavage of SiHa Cells |
title_short | Trichomonas vaginalis Metalloproteinase Induces mTOR Cleavage of SiHa Cells |
title_sort | trichomonas vaginalis metalloproteinase induces mtor cleavage of siha cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4277021/ https://www.ncbi.nlm.nih.gov/pubmed/25548410 http://dx.doi.org/10.3347/kjp.2014.52.6.595 |
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