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Detection of New Delhi metallo beta lactamase-1 (NDM-1) carbapenemase in Pseudomonas aeruginosa in a single centre in southern India

BACKGROUND & OBJECTIVES: New Delhi metallo β-lactamase-1 (NDM-1) producing Pseudomonas aeruginosa isolates are potential threat to human health. This study was conducted to detect the presence of bla(NDM-1) in carbapenem resistant P. aeruginosa in a tertiary care center in southern India. METHOD...

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Autores principales: Shanthi, Mariappan, Sekar, Uma, Kamalanathan, Arunagiri, Sekar, Balaraman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4277142/
https://www.ncbi.nlm.nih.gov/pubmed/25488450
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author Shanthi, Mariappan
Sekar, Uma
Kamalanathan, Arunagiri
Sekar, Balaraman
author_facet Shanthi, Mariappan
Sekar, Uma
Kamalanathan, Arunagiri
Sekar, Balaraman
author_sort Shanthi, Mariappan
collection PubMed
description BACKGROUND & OBJECTIVES: New Delhi metallo β-lactamase-1 (NDM-1) producing Pseudomonas aeruginosa isolates are potential threat to human health. This study was conducted to detect the presence of bla(NDM-1) in carbapenem resistant P. aeruginosa in a tertiary care center in southern India. METHODS: Sixty one carbapenem resistant clinical isolates of a total of 212 P. aeruginosa isolates cultured during the study period were screened for the presence of NDM-1by PCR. Clinical characteristics of the NDM-1 positive isolates were studied and outcome of the patients was followed up. RESULTS: Of the 61 isolates, NDM-1 was detected in four isolates only. These were isolated from patients in the intensive care units and chest medicine ward. The source specimens were pus, sputum, bronchoalveolar lavage and endotracheal aspirate. The NDM-1 producers were susceptible only to polymyxin B. Only one patient responded to polymyxin B therapy, while the others succumbed to the infection. CONCLUSION: These findings reveal that NDM-1 is not a major mechanism mediating carbapenem resistance in P. aeruginosa in this centre. However, continuous surveillance and screening are necessary to prevent their dissemination.
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spelling pubmed-42771422015-01-02 Detection of New Delhi metallo beta lactamase-1 (NDM-1) carbapenemase in Pseudomonas aeruginosa in a single centre in southern India Shanthi, Mariappan Sekar, Uma Kamalanathan, Arunagiri Sekar, Balaraman Indian J Med Res Original Article BACKGROUND & OBJECTIVES: New Delhi metallo β-lactamase-1 (NDM-1) producing Pseudomonas aeruginosa isolates are potential threat to human health. This study was conducted to detect the presence of bla(NDM-1) in carbapenem resistant P. aeruginosa in a tertiary care center in southern India. METHODS: Sixty one carbapenem resistant clinical isolates of a total of 212 P. aeruginosa isolates cultured during the study period were screened for the presence of NDM-1by PCR. Clinical characteristics of the NDM-1 positive isolates were studied and outcome of the patients was followed up. RESULTS: Of the 61 isolates, NDM-1 was detected in four isolates only. These were isolated from patients in the intensive care units and chest medicine ward. The source specimens were pus, sputum, bronchoalveolar lavage and endotracheal aspirate. The NDM-1 producers were susceptible only to polymyxin B. Only one patient responded to polymyxin B therapy, while the others succumbed to the infection. CONCLUSION: These findings reveal that NDM-1 is not a major mechanism mediating carbapenem resistance in P. aeruginosa in this centre. However, continuous surveillance and screening are necessary to prevent their dissemination. Medknow Publications & Media Pvt Ltd 2014-10 /pmc/articles/PMC4277142/ /pubmed/25488450 Text en Copyright: © Indian Journal of Medical Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Shanthi, Mariappan
Sekar, Uma
Kamalanathan, Arunagiri
Sekar, Balaraman
Detection of New Delhi metallo beta lactamase-1 (NDM-1) carbapenemase in Pseudomonas aeruginosa in a single centre in southern India
title Detection of New Delhi metallo beta lactamase-1 (NDM-1) carbapenemase in Pseudomonas aeruginosa in a single centre in southern India
title_full Detection of New Delhi metallo beta lactamase-1 (NDM-1) carbapenemase in Pseudomonas aeruginosa in a single centre in southern India
title_fullStr Detection of New Delhi metallo beta lactamase-1 (NDM-1) carbapenemase in Pseudomonas aeruginosa in a single centre in southern India
title_full_unstemmed Detection of New Delhi metallo beta lactamase-1 (NDM-1) carbapenemase in Pseudomonas aeruginosa in a single centre in southern India
title_short Detection of New Delhi metallo beta lactamase-1 (NDM-1) carbapenemase in Pseudomonas aeruginosa in a single centre in southern India
title_sort detection of new delhi metallo beta lactamase-1 (ndm-1) carbapenemase in pseudomonas aeruginosa in a single centre in southern india
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4277142/
https://www.ncbi.nlm.nih.gov/pubmed/25488450
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