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SPONTANEOUS MUTATION ACCUMULATION IN MULTIPLE STRAINS OF THE GREEN ALGA, CHLAMYDOMONAS REINHARDTII

Estimates of mutational parameters, such as the average fitness effect of a new mutation and the rate at which new genetic variation for fitness is created by mutation, are important for the understanding of many biological processes. However, the causes of interspecific variation in mutational para...

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Autores principales: Morgan, Andrew D, Ness, Rob W, Keightley, Peter D, Colegrave, Nick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wiley Periodicals, Inc 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4277324/
https://www.ncbi.nlm.nih.gov/pubmed/24826801
http://dx.doi.org/10.1111/evo.12448
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author Morgan, Andrew D
Ness, Rob W
Keightley, Peter D
Colegrave, Nick
author_facet Morgan, Andrew D
Ness, Rob W
Keightley, Peter D
Colegrave, Nick
author_sort Morgan, Andrew D
collection PubMed
description Estimates of mutational parameters, such as the average fitness effect of a new mutation and the rate at which new genetic variation for fitness is created by mutation, are important for the understanding of many biological processes. However, the causes of interspecific variation in mutational parameters and the extent to which they vary within species remain largely unknown. We maintained multiple strains of the unicellular eukaryote Chlamydomonas reinhardtii, for approximately 1000 generations under relaxed selection by transferring a single cell every ∼10 generations. Mean fitness of the lines tended to decline with generations of mutation accumulation whereas mutational variance increased. We did not find any evidence for differences among strains in any of the mutational parameters estimated. The overall change in mean fitness per cell division and rate of input of mutational variance per cell division were more similar to values observed in multicellular organisms than to those in other single-celled microbes. However, after taking into account differences in genome size among species, estimates from multicellular organisms and microbes, including our new estimates from C. reinhardtii, become substantially more similar. Thus, we suggest that variation in genome size is an important determinant of interspecific variation in mutational parameters.
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spelling pubmed-42773242014-12-29 SPONTANEOUS MUTATION ACCUMULATION IN MULTIPLE STRAINS OF THE GREEN ALGA, CHLAMYDOMONAS REINHARDTII Morgan, Andrew D Ness, Rob W Keightley, Peter D Colegrave, Nick Evolution Original Articles Estimates of mutational parameters, such as the average fitness effect of a new mutation and the rate at which new genetic variation for fitness is created by mutation, are important for the understanding of many biological processes. However, the causes of interspecific variation in mutational parameters and the extent to which they vary within species remain largely unknown. We maintained multiple strains of the unicellular eukaryote Chlamydomonas reinhardtii, for approximately 1000 generations under relaxed selection by transferring a single cell every ∼10 generations. Mean fitness of the lines tended to decline with generations of mutation accumulation whereas mutational variance increased. We did not find any evidence for differences among strains in any of the mutational parameters estimated. The overall change in mean fitness per cell division and rate of input of mutational variance per cell division were more similar to values observed in multicellular organisms than to those in other single-celled microbes. However, after taking into account differences in genome size among species, estimates from multicellular organisms and microbes, including our new estimates from C. reinhardtii, become substantially more similar. Thus, we suggest that variation in genome size is an important determinant of interspecific variation in mutational parameters. Wiley Periodicals, Inc 2014-09 2014-07-09 /pmc/articles/PMC4277324/ /pubmed/24826801 http://dx.doi.org/10.1111/evo.12448 Text en © 2014 The Authors. Evolution published by Wiley Periodicals, Inc. on behalf of The Society for the Study of Evolution. http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Morgan, Andrew D
Ness, Rob W
Keightley, Peter D
Colegrave, Nick
SPONTANEOUS MUTATION ACCUMULATION IN MULTIPLE STRAINS OF THE GREEN ALGA, CHLAMYDOMONAS REINHARDTII
title SPONTANEOUS MUTATION ACCUMULATION IN MULTIPLE STRAINS OF THE GREEN ALGA, CHLAMYDOMONAS REINHARDTII
title_full SPONTANEOUS MUTATION ACCUMULATION IN MULTIPLE STRAINS OF THE GREEN ALGA, CHLAMYDOMONAS REINHARDTII
title_fullStr SPONTANEOUS MUTATION ACCUMULATION IN MULTIPLE STRAINS OF THE GREEN ALGA, CHLAMYDOMONAS REINHARDTII
title_full_unstemmed SPONTANEOUS MUTATION ACCUMULATION IN MULTIPLE STRAINS OF THE GREEN ALGA, CHLAMYDOMONAS REINHARDTII
title_short SPONTANEOUS MUTATION ACCUMULATION IN MULTIPLE STRAINS OF THE GREEN ALGA, CHLAMYDOMONAS REINHARDTII
title_sort spontaneous mutation accumulation in multiple strains of the green alga, chlamydomonas reinhardtii
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4277324/
https://www.ncbi.nlm.nih.gov/pubmed/24826801
http://dx.doi.org/10.1111/evo.12448
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