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Characterisation of Non-Autoinducing Tropodithietic Acid (TDA) Production from Marine Sponge Pseudovibrio Species
The search for new antimicrobial compounds has gained added momentum in recent years, paralleled by the exponential rise in resistance to most known classes of current antibiotics. While modifications of existing drugs have brought some limited clinical success, there remains a critical need for new...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4278212/ https://www.ncbi.nlm.nih.gov/pubmed/25513851 http://dx.doi.org/10.3390/md12125960 |
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author | Harrington, Catriona Reen, F. Jerry Mooij, Marlies J. Stewart, Fiona A. Chabot, Jean-Baptiste Guerra, Antonio F. Glöckner, Frank O. Nielsen, Kristian F. Gram, Lone Dobson, Alan D. W. Adams, Claire O’Gara, Fergal |
author_facet | Harrington, Catriona Reen, F. Jerry Mooij, Marlies J. Stewart, Fiona A. Chabot, Jean-Baptiste Guerra, Antonio F. Glöckner, Frank O. Nielsen, Kristian F. Gram, Lone Dobson, Alan D. W. Adams, Claire O’Gara, Fergal |
author_sort | Harrington, Catriona |
collection | PubMed |
description | The search for new antimicrobial compounds has gained added momentum in recent years, paralleled by the exponential rise in resistance to most known classes of current antibiotics. While modifications of existing drugs have brought some limited clinical success, there remains a critical need for new classes of antimicrobial compound to which key clinical pathogens will be naive. This has provided the context and impetus to marine biodiscovery programmes that seek to isolate and characterize new activities from the aquatic ecosystem. One new antibiotic to emerge from these initiatives is the antibacterial compound tropodithietic acid (TDA). The aim of this study was to provide insight into the bioactivity of and the factors governing the production of TDA in marine Pseudovibrio isolates from a collection of marine sponges. The TDA produced by these Pseudovibrio isolates exhibited potent antimicrobial activity against a broad spectrum of clinical pathogens, while TDA tolerance was frequent in non-TDA producing marine isolates. Comparative genomics analysis suggested a high degree of conservation among the tda biosynthetic clusters while expression studies revealed coordinated regulation of TDA synthesis upon transition from log to stationary phase growth, which was not induced by TDA itself or by the presence of the C10-acyl homoserine lactone quorum sensing signal molecule. |
format | Online Article Text |
id | pubmed-4278212 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-42782122015-01-08 Characterisation of Non-Autoinducing Tropodithietic Acid (TDA) Production from Marine Sponge Pseudovibrio Species Harrington, Catriona Reen, F. Jerry Mooij, Marlies J. Stewart, Fiona A. Chabot, Jean-Baptiste Guerra, Antonio F. Glöckner, Frank O. Nielsen, Kristian F. Gram, Lone Dobson, Alan D. W. Adams, Claire O’Gara, Fergal Mar Drugs Article The search for new antimicrobial compounds has gained added momentum in recent years, paralleled by the exponential rise in resistance to most known classes of current antibiotics. While modifications of existing drugs have brought some limited clinical success, there remains a critical need for new classes of antimicrobial compound to which key clinical pathogens will be naive. This has provided the context and impetus to marine biodiscovery programmes that seek to isolate and characterize new activities from the aquatic ecosystem. One new antibiotic to emerge from these initiatives is the antibacterial compound tropodithietic acid (TDA). The aim of this study was to provide insight into the bioactivity of and the factors governing the production of TDA in marine Pseudovibrio isolates from a collection of marine sponges. The TDA produced by these Pseudovibrio isolates exhibited potent antimicrobial activity against a broad spectrum of clinical pathogens, while TDA tolerance was frequent in non-TDA producing marine isolates. Comparative genomics analysis suggested a high degree of conservation among the tda biosynthetic clusters while expression studies revealed coordinated regulation of TDA synthesis upon transition from log to stationary phase growth, which was not induced by TDA itself or by the presence of the C10-acyl homoserine lactone quorum sensing signal molecule. MDPI 2014-12-10 /pmc/articles/PMC4278212/ /pubmed/25513851 http://dx.doi.org/10.3390/md12125960 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Harrington, Catriona Reen, F. Jerry Mooij, Marlies J. Stewart, Fiona A. Chabot, Jean-Baptiste Guerra, Antonio F. Glöckner, Frank O. Nielsen, Kristian F. Gram, Lone Dobson, Alan D. W. Adams, Claire O’Gara, Fergal Characterisation of Non-Autoinducing Tropodithietic Acid (TDA) Production from Marine Sponge Pseudovibrio Species |
title | Characterisation of Non-Autoinducing Tropodithietic Acid (TDA) Production from Marine Sponge Pseudovibrio Species |
title_full | Characterisation of Non-Autoinducing Tropodithietic Acid (TDA) Production from Marine Sponge Pseudovibrio Species |
title_fullStr | Characterisation of Non-Autoinducing Tropodithietic Acid (TDA) Production from Marine Sponge Pseudovibrio Species |
title_full_unstemmed | Characterisation of Non-Autoinducing Tropodithietic Acid (TDA) Production from Marine Sponge Pseudovibrio Species |
title_short | Characterisation of Non-Autoinducing Tropodithietic Acid (TDA) Production from Marine Sponge Pseudovibrio Species |
title_sort | characterisation of non-autoinducing tropodithietic acid (tda) production from marine sponge pseudovibrio species |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4278212/ https://www.ncbi.nlm.nih.gov/pubmed/25513851 http://dx.doi.org/10.3390/md12125960 |
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