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Partial Optimization of the 5-Terminal Codon Increased a Recombination Porcine Pancreatic Lipase (opPPL) Expression in Pichia pastoris
Pancreatic lipase plays a key role in intestinal digestion of feed fat, and is often deficient in young animals such as weaning piglets. The objective of this study was to express and characterize a partial codon optimized porcine pancreatic lipase (opPPL). A 537 bp cDNA fragment encoding N-terminus...
Autores principales: | Zhao, Hua, Chen, Dan, Tang, Jiayong, Jia, Gang, Long, Dingbiao, Liu, Guangmang, Chen, Xiaoling, Shang, Haiying |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4278863/ https://www.ncbi.nlm.nih.gov/pubmed/25544987 http://dx.doi.org/10.1371/journal.pone.0114385 |
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