MicroRNA-33b Suppresses Migration and Invasion by Targeting c-Myc in Osteosarcoma Cells
MicroRNAs have emerged as fundamental regulators in gene expression through silencing gene expression at the post-transcriptional and translational levels. Osteosarcoma is the most common type of primary malignant bone tumor and is characterized by complex genetic changes and resistance to conventio...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4278906/ https://www.ncbi.nlm.nih.gov/pubmed/25546234 http://dx.doi.org/10.1371/journal.pone.0115300 |
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author | Xu, Ning Li, Zimu Yu, Zhange Yan, Feng Liu, Yang Lu, Xiaofeng Yang, Weiliang |
author_facet | Xu, Ning Li, Zimu Yu, Zhange Yan, Feng Liu, Yang Lu, Xiaofeng Yang, Weiliang |
author_sort | Xu, Ning |
collection | PubMed |
description | MicroRNAs have emerged as fundamental regulators in gene expression through silencing gene expression at the post-transcriptional and translational levels. Osteosarcoma is the most common type of primary malignant bone tumor and is characterized by complex genetic changes and resistance to conventional treatments. In our study, the role of miR-33b in the progression and metastasis of osteosarcoma was investigated. Our results showed that miR-33b was significantly downregulated in osteosarcoma tissue and cell lines. Overexpression of miR-33b significantly inhibited cell proliferation, migration, and invasion in the MG-63 osteosarcoma cell line. Moreover, we also showed that c-Myc was negatively regulated by miR-33b at the posttranscriptional level, via a specific target site within the 3′UTR. Overexpression of c-Myc impaired miR-33b-induced inhibition of proliferation and invasion in osteosarcoma cells. The expression of c-Myc was frequently downregulated in osteosarcoma tumors and cell lines and was inversely correlated with miR-33b expression. Thus, our findings suggest that miR-33b inhibits osteosarcoma cells migration and invasion by targeting the c-Myc gene, acting as tumor suppressor. The findings of this study contribute to current understanding of the functions of miR-33b in osteosarcoma. |
format | Online Article Text |
id | pubmed-4278906 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-42789062015-01-05 MicroRNA-33b Suppresses Migration and Invasion by Targeting c-Myc in Osteosarcoma Cells Xu, Ning Li, Zimu Yu, Zhange Yan, Feng Liu, Yang Lu, Xiaofeng Yang, Weiliang PLoS One Research Article MicroRNAs have emerged as fundamental regulators in gene expression through silencing gene expression at the post-transcriptional and translational levels. Osteosarcoma is the most common type of primary malignant bone tumor and is characterized by complex genetic changes and resistance to conventional treatments. In our study, the role of miR-33b in the progression and metastasis of osteosarcoma was investigated. Our results showed that miR-33b was significantly downregulated in osteosarcoma tissue and cell lines. Overexpression of miR-33b significantly inhibited cell proliferation, migration, and invasion in the MG-63 osteosarcoma cell line. Moreover, we also showed that c-Myc was negatively regulated by miR-33b at the posttranscriptional level, via a specific target site within the 3′UTR. Overexpression of c-Myc impaired miR-33b-induced inhibition of proliferation and invasion in osteosarcoma cells. The expression of c-Myc was frequently downregulated in osteosarcoma tumors and cell lines and was inversely correlated with miR-33b expression. Thus, our findings suggest that miR-33b inhibits osteosarcoma cells migration and invasion by targeting the c-Myc gene, acting as tumor suppressor. The findings of this study contribute to current understanding of the functions of miR-33b in osteosarcoma. Public Library of Science 2014-12-29 /pmc/articles/PMC4278906/ /pubmed/25546234 http://dx.doi.org/10.1371/journal.pone.0115300 Text en © 2014 Xu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Xu, Ning Li, Zimu Yu, Zhange Yan, Feng Liu, Yang Lu, Xiaofeng Yang, Weiliang MicroRNA-33b Suppresses Migration and Invasion by Targeting c-Myc in Osteosarcoma Cells |
title | MicroRNA-33b Suppresses Migration and Invasion by Targeting c-Myc in Osteosarcoma Cells |
title_full | MicroRNA-33b Suppresses Migration and Invasion by Targeting c-Myc in Osteosarcoma Cells |
title_fullStr | MicroRNA-33b Suppresses Migration and Invasion by Targeting c-Myc in Osteosarcoma Cells |
title_full_unstemmed | MicroRNA-33b Suppresses Migration and Invasion by Targeting c-Myc in Osteosarcoma Cells |
title_short | MicroRNA-33b Suppresses Migration and Invasion by Targeting c-Myc in Osteosarcoma Cells |
title_sort | microrna-33b suppresses migration and invasion by targeting c-myc in osteosarcoma cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4278906/ https://www.ncbi.nlm.nih.gov/pubmed/25546234 http://dx.doi.org/10.1371/journal.pone.0115300 |
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