MicroRNA-33b Suppresses Migration and Invasion by Targeting c-Myc in Osteosarcoma Cells

MicroRNAs have emerged as fundamental regulators in gene expression through silencing gene expression at the post-transcriptional and translational levels. Osteosarcoma is the most common type of primary malignant bone tumor and is characterized by complex genetic changes and resistance to conventio...

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Autores principales: Xu, Ning, Li, Zimu, Yu, Zhange, Yan, Feng, Liu, Yang, Lu, Xiaofeng, Yang, Weiliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4278906/
https://www.ncbi.nlm.nih.gov/pubmed/25546234
http://dx.doi.org/10.1371/journal.pone.0115300
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author Xu, Ning
Li, Zimu
Yu, Zhange
Yan, Feng
Liu, Yang
Lu, Xiaofeng
Yang, Weiliang
author_facet Xu, Ning
Li, Zimu
Yu, Zhange
Yan, Feng
Liu, Yang
Lu, Xiaofeng
Yang, Weiliang
author_sort Xu, Ning
collection PubMed
description MicroRNAs have emerged as fundamental regulators in gene expression through silencing gene expression at the post-transcriptional and translational levels. Osteosarcoma is the most common type of primary malignant bone tumor and is characterized by complex genetic changes and resistance to conventional treatments. In our study, the role of miR-33b in the progression and metastasis of osteosarcoma was investigated. Our results showed that miR-33b was significantly downregulated in osteosarcoma tissue and cell lines. Overexpression of miR-33b significantly inhibited cell proliferation, migration, and invasion in the MG-63 osteosarcoma cell line. Moreover, we also showed that c-Myc was negatively regulated by miR-33b at the posttranscriptional level, via a specific target site within the 3′UTR. Overexpression of c-Myc impaired miR-33b-induced inhibition of proliferation and invasion in osteosarcoma cells. The expression of c-Myc was frequently downregulated in osteosarcoma tumors and cell lines and was inversely correlated with miR-33b expression. Thus, our findings suggest that miR-33b inhibits osteosarcoma cells migration and invasion by targeting the c-Myc gene, acting as tumor suppressor. The findings of this study contribute to current understanding of the functions of miR-33b in osteosarcoma.
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spelling pubmed-42789062015-01-05 MicroRNA-33b Suppresses Migration and Invasion by Targeting c-Myc in Osteosarcoma Cells Xu, Ning Li, Zimu Yu, Zhange Yan, Feng Liu, Yang Lu, Xiaofeng Yang, Weiliang PLoS One Research Article MicroRNAs have emerged as fundamental regulators in gene expression through silencing gene expression at the post-transcriptional and translational levels. Osteosarcoma is the most common type of primary malignant bone tumor and is characterized by complex genetic changes and resistance to conventional treatments. In our study, the role of miR-33b in the progression and metastasis of osteosarcoma was investigated. Our results showed that miR-33b was significantly downregulated in osteosarcoma tissue and cell lines. Overexpression of miR-33b significantly inhibited cell proliferation, migration, and invasion in the MG-63 osteosarcoma cell line. Moreover, we also showed that c-Myc was negatively regulated by miR-33b at the posttranscriptional level, via a specific target site within the 3′UTR. Overexpression of c-Myc impaired miR-33b-induced inhibition of proliferation and invasion in osteosarcoma cells. The expression of c-Myc was frequently downregulated in osteosarcoma tumors and cell lines and was inversely correlated with miR-33b expression. Thus, our findings suggest that miR-33b inhibits osteosarcoma cells migration and invasion by targeting the c-Myc gene, acting as tumor suppressor. The findings of this study contribute to current understanding of the functions of miR-33b in osteosarcoma. Public Library of Science 2014-12-29 /pmc/articles/PMC4278906/ /pubmed/25546234 http://dx.doi.org/10.1371/journal.pone.0115300 Text en © 2014 Xu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Xu, Ning
Li, Zimu
Yu, Zhange
Yan, Feng
Liu, Yang
Lu, Xiaofeng
Yang, Weiliang
MicroRNA-33b Suppresses Migration and Invasion by Targeting c-Myc in Osteosarcoma Cells
title MicroRNA-33b Suppresses Migration and Invasion by Targeting c-Myc in Osteosarcoma Cells
title_full MicroRNA-33b Suppresses Migration and Invasion by Targeting c-Myc in Osteosarcoma Cells
title_fullStr MicroRNA-33b Suppresses Migration and Invasion by Targeting c-Myc in Osteosarcoma Cells
title_full_unstemmed MicroRNA-33b Suppresses Migration and Invasion by Targeting c-Myc in Osteosarcoma Cells
title_short MicroRNA-33b Suppresses Migration and Invasion by Targeting c-Myc in Osteosarcoma Cells
title_sort microrna-33b suppresses migration and invasion by targeting c-myc in osteosarcoma cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4278906/
https://www.ncbi.nlm.nih.gov/pubmed/25546234
http://dx.doi.org/10.1371/journal.pone.0115300
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