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The contractile ring coordinates curvature-dependent septum assembly during fission yeast cytokinesis
The functions of the actin-myosin–based contractile ring in cytokinesis remain to be elucidated. Recent findings show that in the fission yeast Schizosaccharomyces pombe, cleavage furrow ingression is driven by polymerization of cell wall fibers outside the plasma membrane, not by the contractile ri...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4279231/ https://www.ncbi.nlm.nih.gov/pubmed/25355954 http://dx.doi.org/10.1091/mbc.E14-10-1441 |
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author | Zhou, Zhou Munteanu, Emilia Laura He, Jun Ursell, Tristan Bathe, Mark Huang, Kerwyn Casey Chang, Fred |
author_facet | Zhou, Zhou Munteanu, Emilia Laura He, Jun Ursell, Tristan Bathe, Mark Huang, Kerwyn Casey Chang, Fred |
author_sort | Zhou, Zhou |
collection | PubMed |
description | The functions of the actin-myosin–based contractile ring in cytokinesis remain to be elucidated. Recent findings show that in the fission yeast Schizosaccharomyces pombe, cleavage furrow ingression is driven by polymerization of cell wall fibers outside the plasma membrane, not by the contractile ring. Here we show that one function of the ring is to spatially coordinate septum cell wall assembly. We develop an improved method for live-cell imaging of the division apparatus by orienting the rod-shaped cells vertically using microfabricated wells. We observe that the septum hole and ring are circular and centered in wild-type cells and that in the absence of a functional ring, the septum continues to ingress but in a disorganized and asymmetric manner. By manipulating the cleavage furrow into different shapes, we show that the ring promotes local septum growth in a curvature-dependent manner, allowing even a misshapen septum to grow into a more regular shape. This curvature-dependent growth suggests a model in which contractile forces of the ring shape the septum cell wall by stimulating the cell wall machinery in a mechanosensitive manner. Mechanical regulation of the cell wall assembly may have general relevance to the morphogenesis of walled cells. |
format | Online Article Text |
id | pubmed-4279231 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-42792312015-03-16 The contractile ring coordinates curvature-dependent septum assembly during fission yeast cytokinesis Zhou, Zhou Munteanu, Emilia Laura He, Jun Ursell, Tristan Bathe, Mark Huang, Kerwyn Casey Chang, Fred Mol Biol Cell Articles The functions of the actin-myosin–based contractile ring in cytokinesis remain to be elucidated. Recent findings show that in the fission yeast Schizosaccharomyces pombe, cleavage furrow ingression is driven by polymerization of cell wall fibers outside the plasma membrane, not by the contractile ring. Here we show that one function of the ring is to spatially coordinate septum cell wall assembly. We develop an improved method for live-cell imaging of the division apparatus by orienting the rod-shaped cells vertically using microfabricated wells. We observe that the septum hole and ring are circular and centered in wild-type cells and that in the absence of a functional ring, the septum continues to ingress but in a disorganized and asymmetric manner. By manipulating the cleavage furrow into different shapes, we show that the ring promotes local septum growth in a curvature-dependent manner, allowing even a misshapen septum to grow into a more regular shape. This curvature-dependent growth suggests a model in which contractile forces of the ring shape the septum cell wall by stimulating the cell wall machinery in a mechanosensitive manner. Mechanical regulation of the cell wall assembly may have general relevance to the morphogenesis of walled cells. The American Society for Cell Biology 2015-01-01 /pmc/articles/PMC4279231/ /pubmed/25355954 http://dx.doi.org/10.1091/mbc.E14-10-1441 Text en © 2015 Zhou et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. |
spellingShingle | Articles Zhou, Zhou Munteanu, Emilia Laura He, Jun Ursell, Tristan Bathe, Mark Huang, Kerwyn Casey Chang, Fred The contractile ring coordinates curvature-dependent septum assembly during fission yeast cytokinesis |
title | The contractile ring coordinates curvature-dependent septum assembly during fission yeast cytokinesis |
title_full | The contractile ring coordinates curvature-dependent septum assembly during fission yeast cytokinesis |
title_fullStr | The contractile ring coordinates curvature-dependent septum assembly during fission yeast cytokinesis |
title_full_unstemmed | The contractile ring coordinates curvature-dependent septum assembly during fission yeast cytokinesis |
title_short | The contractile ring coordinates curvature-dependent septum assembly during fission yeast cytokinesis |
title_sort | contractile ring coordinates curvature-dependent septum assembly during fission yeast cytokinesis |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4279231/ https://www.ncbi.nlm.nih.gov/pubmed/25355954 http://dx.doi.org/10.1091/mbc.E14-10-1441 |
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