A Coumarin-Based Fluorescent Probe as a Central Nervous System Disease Biomarker

Homocysteine and methylmalonic acid are important biomarkers for diseases associated with an impaired central nervous system (CNS). A new chemoassay utilizing coumarin-based fluorescent probe 1 to detect the levels of homocysteine is successfully implemented using Parkinson's disease (PD) patie...

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Autores principales: Yap, Ann-Chee, Mahamad, Ummi Affah, Lim, Shen-Yang, Kim, Hae-Jo, Choo, Yeun-Mun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4279527/
https://www.ncbi.nlm.nih.gov/pubmed/25390405
http://dx.doi.org/10.3390/s141121140
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author Yap, Ann-Chee
Mahamad, Ummi Affah
Lim, Shen-Yang
Kim, Hae-Jo
Choo, Yeun-Mun
author_facet Yap, Ann-Chee
Mahamad, Ummi Affah
Lim, Shen-Yang
Kim, Hae-Jo
Choo, Yeun-Mun
author_sort Yap, Ann-Chee
collection PubMed
description Homocysteine and methylmalonic acid are important biomarkers for diseases associated with an impaired central nervous system (CNS). A new chemoassay utilizing coumarin-based fluorescent probe 1 to detect the levels of homocysteine is successfully implemented using Parkinson's disease (PD) patients' blood serum. In addition, a rapid identification of homocysteine and methylmalonic acid levels in blood serum of PD patients was also performed using the liquid chromatography-mass spectrometry (LC-MS). The results obtained from both analyses were in agreement. The new chemoassay utilizing coumarin-based fluorescent probe 1 offers a cost- and time-effective method to identify the biomarkers in CNS patients.
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spelling pubmed-42795272015-01-15 A Coumarin-Based Fluorescent Probe as a Central Nervous System Disease Biomarker Yap, Ann-Chee Mahamad, Ummi Affah Lim, Shen-Yang Kim, Hae-Jo Choo, Yeun-Mun Sensors (Basel) Article Homocysteine and methylmalonic acid are important biomarkers for diseases associated with an impaired central nervous system (CNS). A new chemoassay utilizing coumarin-based fluorescent probe 1 to detect the levels of homocysteine is successfully implemented using Parkinson's disease (PD) patients' blood serum. In addition, a rapid identification of homocysteine and methylmalonic acid levels in blood serum of PD patients was also performed using the liquid chromatography-mass spectrometry (LC-MS). The results obtained from both analyses were in agreement. The new chemoassay utilizing coumarin-based fluorescent probe 1 offers a cost- and time-effective method to identify the biomarkers in CNS patients. MDPI 2014-11-10 /pmc/articles/PMC4279527/ /pubmed/25390405 http://dx.doi.org/10.3390/s141121140 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Yap, Ann-Chee
Mahamad, Ummi Affah
Lim, Shen-Yang
Kim, Hae-Jo
Choo, Yeun-Mun
A Coumarin-Based Fluorescent Probe as a Central Nervous System Disease Biomarker
title A Coumarin-Based Fluorescent Probe as a Central Nervous System Disease Biomarker
title_full A Coumarin-Based Fluorescent Probe as a Central Nervous System Disease Biomarker
title_fullStr A Coumarin-Based Fluorescent Probe as a Central Nervous System Disease Biomarker
title_full_unstemmed A Coumarin-Based Fluorescent Probe as a Central Nervous System Disease Biomarker
title_short A Coumarin-Based Fluorescent Probe as a Central Nervous System Disease Biomarker
title_sort coumarin-based fluorescent probe as a central nervous system disease biomarker
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4279527/
https://www.ncbi.nlm.nih.gov/pubmed/25390405
http://dx.doi.org/10.3390/s141121140
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