Synthesis of a Cu(2+)-Selective Probe Derived from Rhodamine and Its Application in Cell Imaging

A new fluorescent probe P based on rhodamine for Cu(2+) was synthesized and characterized. The new probe P showed high selectivity to Cu(2+) over other tested metal ions. With optimal conditions, the proposed probe P worked in a wide linear range of 1.0 × 10(−6)−1.0 × 10(−5) M with a detection limit...

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Detalles Bibliográficos
Autores principales: Yu, Chunwei, Wen, Yingying, Zhang, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4279538/
https://www.ncbi.nlm.nih.gov/pubmed/25397918
http://dx.doi.org/10.3390/s141121375
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author Yu, Chunwei
Wen, Yingying
Zhang, Jun
author_facet Yu, Chunwei
Wen, Yingying
Zhang, Jun
author_sort Yu, Chunwei
collection PubMed
description A new fluorescent probe P based on rhodamine for Cu(2+) was synthesized and characterized. The new probe P showed high selectivity to Cu(2+) over other tested metal ions. With optimal conditions, the proposed probe P worked in a wide linear range of 1.0 × 10(−6)−1.0 × 10(−5) M with a detection limit of 3.3 × 10(−7) M Cu(2+) in ethanol-water solution (9:1, v:v, 20 mM HEPES, pH 7.0). Furthermore, it has been used for imaging of Cu(2+) in living cells with satisfying results.
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spelling pubmed-42795382015-01-15 Synthesis of a Cu(2+)-Selective Probe Derived from Rhodamine and Its Application in Cell Imaging Yu, Chunwei Wen, Yingying Zhang, Jun Sensors (Basel) Article A new fluorescent probe P based on rhodamine for Cu(2+) was synthesized and characterized. The new probe P showed high selectivity to Cu(2+) over other tested metal ions. With optimal conditions, the proposed probe P worked in a wide linear range of 1.0 × 10(−6)−1.0 × 10(−5) M with a detection limit of 3.3 × 10(−7) M Cu(2+) in ethanol-water solution (9:1, v:v, 20 mM HEPES, pH 7.0). Furthermore, it has been used for imaging of Cu(2+) in living cells with satisfying results. MDPI 2014-11-12 /pmc/articles/PMC4279538/ /pubmed/25397918 http://dx.doi.org/10.3390/s141121375 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Yu, Chunwei
Wen, Yingying
Zhang, Jun
Synthesis of a Cu(2+)-Selective Probe Derived from Rhodamine and Its Application in Cell Imaging
title Synthesis of a Cu(2+)-Selective Probe Derived from Rhodamine and Its Application in Cell Imaging
title_full Synthesis of a Cu(2+)-Selective Probe Derived from Rhodamine and Its Application in Cell Imaging
title_fullStr Synthesis of a Cu(2+)-Selective Probe Derived from Rhodamine and Its Application in Cell Imaging
title_full_unstemmed Synthesis of a Cu(2+)-Selective Probe Derived from Rhodamine and Its Application in Cell Imaging
title_short Synthesis of a Cu(2+)-Selective Probe Derived from Rhodamine and Its Application in Cell Imaging
title_sort synthesis of a cu(2+)-selective probe derived from rhodamine and its application in cell imaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4279538/
https://www.ncbi.nlm.nih.gov/pubmed/25397918
http://dx.doi.org/10.3390/s141121375
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AT wenyingying synthesisofacu2selectiveprobederivedfromrhodamineanditsapplicationincellimaging
AT zhangjun synthesisofacu2selectiveprobederivedfromrhodamineanditsapplicationincellimaging

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