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Molecular determination of abundance of infection with Sarcocystis species in slaughtered sheep of Urmia, Iran

Sarcocystis is one of the most prevalent parasites of domestic ruminants worldwide. This study was aimed to determine prevalence of Sarcocystis infection and molecular discrimination of Sarcocystis gigantea and Sarcocystis medusiformis infecting domestic sheep. Tissue samples from 638 sheep slaughte...

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Autores principales: Farhang-Pajuh, Farhad, Yakhchali, Mohammad, Mardani, Karim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Urmia University Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4279651/
https://www.ncbi.nlm.nih.gov/pubmed/25568716
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author Farhang-Pajuh, Farhad
Yakhchali, Mohammad
Mardani, Karim
author_facet Farhang-Pajuh, Farhad
Yakhchali, Mohammad
Mardani, Karim
author_sort Farhang-Pajuh, Farhad
collection PubMed
description Sarcocystis is one of the most prevalent parasites of domestic ruminants worldwide. This study was aimed to determine prevalence of Sarcocystis infection and molecular discrimination of Sarcocystis gigantea and Sarcocystis medusiformis infecting domestic sheep. Tissue samples from 638 sheep slaughtered at Urmia abattoir were randomly collected from February 2011 to January 2012. Genomic DNA extraction and polymerase chain reaction (PCR) was performed to amplify a 964 bp fragment of nuclear 18S rRNA gene. The PCR products were subjected to digestion with endonuclease MboII and/or MvaI for discriminating S. medusiformis and S. gigantea. Results indicated that the overall prevalence of Sarcocystis unspecified species was 36.83% (235/638) in which male (7.63%, 38/498) and female (35.00%, 49/140) sheep over 4 years-old had the highest prevalence. There was no significant difference between prevalence of macrosarcocysts and sex. Two macrosarcocysts forms were found as fat (27.90%, 178/638) and thin (8.93%, 57/638) in striated muscles. There was significant difference between frequency of macrosarcocysts and body distribution. Mixed infection with both fat and thin macrosarcocysts was also found in 11.13% (71/638) of infected sheep. There was no significant difference regarding the prevalence of mixed infection in both age classes. The PCR-RFLP patterns showed that fat sarcocysts were S. gigantea (29.31%, 187/638) and thin sarcocysts were S. medusiformis (7.52%, 48/638). It was concluded that ovine Sarcocystis infection was prevalent in Urmia and a combination of conventional methods and molecular study for sheep sarcocysts could be informative.
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spelling pubmed-42796512015-01-07 Molecular determination of abundance of infection with Sarcocystis species in slaughtered sheep of Urmia, Iran Farhang-Pajuh, Farhad Yakhchali, Mohammad Mardani, Karim Vet Res Forum Original Article Sarcocystis is one of the most prevalent parasites of domestic ruminants worldwide. This study was aimed to determine prevalence of Sarcocystis infection and molecular discrimination of Sarcocystis gigantea and Sarcocystis medusiformis infecting domestic sheep. Tissue samples from 638 sheep slaughtered at Urmia abattoir were randomly collected from February 2011 to January 2012. Genomic DNA extraction and polymerase chain reaction (PCR) was performed to amplify a 964 bp fragment of nuclear 18S rRNA gene. The PCR products were subjected to digestion with endonuclease MboII and/or MvaI for discriminating S. medusiformis and S. gigantea. Results indicated that the overall prevalence of Sarcocystis unspecified species was 36.83% (235/638) in which male (7.63%, 38/498) and female (35.00%, 49/140) sheep over 4 years-old had the highest prevalence. There was no significant difference between prevalence of macrosarcocysts and sex. Two macrosarcocysts forms were found as fat (27.90%, 178/638) and thin (8.93%, 57/638) in striated muscles. There was significant difference between frequency of macrosarcocysts and body distribution. Mixed infection with both fat and thin macrosarcocysts was also found in 11.13% (71/638) of infected sheep. There was no significant difference regarding the prevalence of mixed infection in both age classes. The PCR-RFLP patterns showed that fat sarcocysts were S. gigantea (29.31%, 187/638) and thin sarcocysts were S. medusiformis (7.52%, 48/638). It was concluded that ovine Sarcocystis infection was prevalent in Urmia and a combination of conventional methods and molecular study for sheep sarcocysts could be informative. Urmia University Press 2014 /pmc/articles/PMC4279651/ /pubmed/25568716 Text en © 2014 Urmia University. All rights reserved. This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Farhang-Pajuh, Farhad
Yakhchali, Mohammad
Mardani, Karim
Molecular determination of abundance of infection with Sarcocystis species in slaughtered sheep of Urmia, Iran
title Molecular determination of abundance of infection with Sarcocystis species in slaughtered sheep of Urmia, Iran
title_full Molecular determination of abundance of infection with Sarcocystis species in slaughtered sheep of Urmia, Iran
title_fullStr Molecular determination of abundance of infection with Sarcocystis species in slaughtered sheep of Urmia, Iran
title_full_unstemmed Molecular determination of abundance of infection with Sarcocystis species in slaughtered sheep of Urmia, Iran
title_short Molecular determination of abundance of infection with Sarcocystis species in slaughtered sheep of Urmia, Iran
title_sort molecular determination of abundance of infection with sarcocystis species in slaughtered sheep of urmia, iran
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4279651/
https://www.ncbi.nlm.nih.gov/pubmed/25568716
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