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Toxicity of 100 nm zinc oxide nanoparticles: a report of 90-day repeated oral administration in Sprague Dawley rats

Nanoparticles (NPs) are used commercially in health and fitness fields, but information about the toxicity and mechanisms underlying the toxic effects of NPs is still very limited. The aim of this study is to investigate the toxic effect(s) of 100 nm negatively (ZnO(AE100[−])) or positively (ZnO(AE1...

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Autores principales: Kim, Yu-Ri, Park, Jong-Il, Lee, Eun Jeong, Park, Sung Ha, Seong, Nak-won, Kim, Jun-Ho, Kim, Geon-Yong, Meang, Eun-Ho, Hong, Jeong-Sup, Kim, Su-Hyon, Koh, Sang-Bum, Kim, Min-Seok, Kim, Cheol-Su, Kim, Soo-Ki, Son, Sang Wook, Seo, Young Rok, Kang, Boo Hyon, Han, Beom Seok, An, Seong Soo A, Yun, Hyo-In, Kim, Meyoung-Kon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4279774/
https://www.ncbi.nlm.nih.gov/pubmed/25565830
http://dx.doi.org/10.2147/IJN.S57928
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author Kim, Yu-Ri
Park, Jong-Il
Lee, Eun Jeong
Park, Sung Ha
Seong, Nak-won
Kim, Jun-Ho
Kim, Geon-Yong
Meang, Eun-Ho
Hong, Jeong-Sup
Kim, Su-Hyon
Koh, Sang-Bum
Kim, Min-Seok
Kim, Cheol-Su
Kim, Soo-Ki
Son, Sang Wook
Seo, Young Rok
Kang, Boo Hyon
Han, Beom Seok
An, Seong Soo A
Yun, Hyo-In
Kim, Meyoung-Kon
author_facet Kim, Yu-Ri
Park, Jong-Il
Lee, Eun Jeong
Park, Sung Ha
Seong, Nak-won
Kim, Jun-Ho
Kim, Geon-Yong
Meang, Eun-Ho
Hong, Jeong-Sup
Kim, Su-Hyon
Koh, Sang-Bum
Kim, Min-Seok
Kim, Cheol-Su
Kim, Soo-Ki
Son, Sang Wook
Seo, Young Rok
Kang, Boo Hyon
Han, Beom Seok
An, Seong Soo A
Yun, Hyo-In
Kim, Meyoung-Kon
author_sort Kim, Yu-Ri
collection PubMed
description Nanoparticles (NPs) are used commercially in health and fitness fields, but information about the toxicity and mechanisms underlying the toxic effects of NPs is still very limited. The aim of this study is to investigate the toxic effect(s) of 100 nm negatively (ZnO(AE100[−])) or positively (ZnO(AE100[+])) charged zinc oxide (ZnO) NPs administered by gavage in Sprague Dawley rats, to establish a no observed adverse effect level, and to identify target organ(s). After verification of the primary particle size, morphology, hydrodynamic size, and zeta potential of each test article, we performed a 90-day study according to Organisation for Economic Co-operation and Development test guideline 408. For the 90-day study, the high dose was set at 500 mg/kg and the middle and low doses were set at 125 mg/kg and 31.25 mg/kg, respectively. Both ZnO NPs had significant changes in hematological and blood biochemical analysis, which could correlate with anemia-related parameters, in the 500 mg/kg groups of both sexes. Histopathological examination showed significant adverse effects (by both test articles) in the stomach, pancreas, eye, and prostate gland tissues, but the particle charge did not affect the tendency or the degree of the lesions. We speculate that this inflammatory damage might result from continuous irritation caused by both test articles. Therefore, the target organs for both ZnO(AE100(−)) and ZnO(AE100(+)) are considered to be the stomach, pancreas, eye, and prostate gland. Also, the no observed adverse effect level for both test articles was identified as 31.25 mg/kg for both sexes, because the adverse effects were observed at all doses greater than 125 mg/kg.
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spelling pubmed-42797742015-01-06 Toxicity of 100 nm zinc oxide nanoparticles: a report of 90-day repeated oral administration in Sprague Dawley rats Kim, Yu-Ri Park, Jong-Il Lee, Eun Jeong Park, Sung Ha Seong, Nak-won Kim, Jun-Ho Kim, Geon-Yong Meang, Eun-Ho Hong, Jeong-Sup Kim, Su-Hyon Koh, Sang-Bum Kim, Min-Seok Kim, Cheol-Su Kim, Soo-Ki Son, Sang Wook Seo, Young Rok Kang, Boo Hyon Han, Beom Seok An, Seong Soo A Yun, Hyo-In Kim, Meyoung-Kon Int J Nanomedicine Original Research Nanoparticles (NPs) are used commercially in health and fitness fields, but information about the toxicity and mechanisms underlying the toxic effects of NPs is still very limited. The aim of this study is to investigate the toxic effect(s) of 100 nm negatively (ZnO(AE100[−])) or positively (ZnO(AE100[+])) charged zinc oxide (ZnO) NPs administered by gavage in Sprague Dawley rats, to establish a no observed adverse effect level, and to identify target organ(s). After verification of the primary particle size, morphology, hydrodynamic size, and zeta potential of each test article, we performed a 90-day study according to Organisation for Economic Co-operation and Development test guideline 408. For the 90-day study, the high dose was set at 500 mg/kg and the middle and low doses were set at 125 mg/kg and 31.25 mg/kg, respectively. Both ZnO NPs had significant changes in hematological and blood biochemical analysis, which could correlate with anemia-related parameters, in the 500 mg/kg groups of both sexes. Histopathological examination showed significant adverse effects (by both test articles) in the stomach, pancreas, eye, and prostate gland tissues, but the particle charge did not affect the tendency or the degree of the lesions. We speculate that this inflammatory damage might result from continuous irritation caused by both test articles. Therefore, the target organs for both ZnO(AE100(−)) and ZnO(AE100(+)) are considered to be the stomach, pancreas, eye, and prostate gland. Also, the no observed adverse effect level for both test articles was identified as 31.25 mg/kg for both sexes, because the adverse effects were observed at all doses greater than 125 mg/kg. Dove Medical Press 2014-12-15 /pmc/articles/PMC4279774/ /pubmed/25565830 http://dx.doi.org/10.2147/IJN.S57928 Text en © 2014 Kim et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Kim, Yu-Ri
Park, Jong-Il
Lee, Eun Jeong
Park, Sung Ha
Seong, Nak-won
Kim, Jun-Ho
Kim, Geon-Yong
Meang, Eun-Ho
Hong, Jeong-Sup
Kim, Su-Hyon
Koh, Sang-Bum
Kim, Min-Seok
Kim, Cheol-Su
Kim, Soo-Ki
Son, Sang Wook
Seo, Young Rok
Kang, Boo Hyon
Han, Beom Seok
An, Seong Soo A
Yun, Hyo-In
Kim, Meyoung-Kon
Toxicity of 100 nm zinc oxide nanoparticles: a report of 90-day repeated oral administration in Sprague Dawley rats
title Toxicity of 100 nm zinc oxide nanoparticles: a report of 90-day repeated oral administration in Sprague Dawley rats
title_full Toxicity of 100 nm zinc oxide nanoparticles: a report of 90-day repeated oral administration in Sprague Dawley rats
title_fullStr Toxicity of 100 nm zinc oxide nanoparticles: a report of 90-day repeated oral administration in Sprague Dawley rats
title_full_unstemmed Toxicity of 100 nm zinc oxide nanoparticles: a report of 90-day repeated oral administration in Sprague Dawley rats
title_short Toxicity of 100 nm zinc oxide nanoparticles: a report of 90-day repeated oral administration in Sprague Dawley rats
title_sort toxicity of 100 nm zinc oxide nanoparticles: a report of 90-day repeated oral administration in sprague dawley rats
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4279774/
https://www.ncbi.nlm.nih.gov/pubmed/25565830
http://dx.doi.org/10.2147/IJN.S57928
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