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The Incidence of Sexually Dimorphic Gene Expression Varies Greatly between Tissues in the Rat
The sexually dimorphic expression of genes across 26 somatic rat tissues was using Affymetrix RAE-230 genechips. We considered probesets to be sexually dimorphically expressed (SDE) if they were measurably expressed above background in at least one sex, there was at least a two-fold difference in ex...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4280129/ https://www.ncbi.nlm.nih.gov/pubmed/25548914 http://dx.doi.org/10.1371/journal.pone.0115792 |
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author | Huby, Russell D. J. Glaves, Philip Jackson, Richard |
author_facet | Huby, Russell D. J. Glaves, Philip Jackson, Richard |
author_sort | Huby, Russell D. J. |
collection | PubMed |
description | The sexually dimorphic expression of genes across 26 somatic rat tissues was using Affymetrix RAE-230 genechips. We considered probesets to be sexually dimorphically expressed (SDE) if they were measurably expressed above background in at least one sex, there was at least a two-fold difference in expression (dimorphism) between the sexes, and the differences were statistically significant after correcting for false discovery. 14.5% of expressed probesets were SDE in at least one tissue, with higher expression nearly twice as prevalent in males compared to females. Most were SDE in a single tissue. Surprisingly, nearly half of the probesets that were (SDE) in multiple tissues were oppositely sex biased in different tissues, and most SDE probesets were also expressed without sex bias in other tissues. Two genes were widely SDE: Xist (female-only) and Eif2s3y (male-only). The frequency of SDE probesets varied widely between tissues, and was highest in the duodenum (6.2%), whilst less than 0.05% in over half of the surveyed tissues. The occurrence of SDE probesets was not strongly correlated between tissues. Within individual tissues, however, relational networks of SDE genes were identified. In the liver, networks relating to differential metabolism between the sexes were seen. The estrogen receptor was implicated in differential gene expression in the duodenum. To conclude, sexually dimorphic gene expression is common, but highly tissue-dependent. Sexually dimorphic gene expression may provide insights into mechanisms underlying phenotypic sex differences. Online data are provided as a resource for further analyses (GEO reference GSE63362). |
format | Online Article Text |
id | pubmed-4280129 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-42801292015-01-07 The Incidence of Sexually Dimorphic Gene Expression Varies Greatly between Tissues in the Rat Huby, Russell D. J. Glaves, Philip Jackson, Richard PLoS One Research Article The sexually dimorphic expression of genes across 26 somatic rat tissues was using Affymetrix RAE-230 genechips. We considered probesets to be sexually dimorphically expressed (SDE) if they were measurably expressed above background in at least one sex, there was at least a two-fold difference in expression (dimorphism) between the sexes, and the differences were statistically significant after correcting for false discovery. 14.5% of expressed probesets were SDE in at least one tissue, with higher expression nearly twice as prevalent in males compared to females. Most were SDE in a single tissue. Surprisingly, nearly half of the probesets that were (SDE) in multiple tissues were oppositely sex biased in different tissues, and most SDE probesets were also expressed without sex bias in other tissues. Two genes were widely SDE: Xist (female-only) and Eif2s3y (male-only). The frequency of SDE probesets varied widely between tissues, and was highest in the duodenum (6.2%), whilst less than 0.05% in over half of the surveyed tissues. The occurrence of SDE probesets was not strongly correlated between tissues. Within individual tissues, however, relational networks of SDE genes were identified. In the liver, networks relating to differential metabolism between the sexes were seen. The estrogen receptor was implicated in differential gene expression in the duodenum. To conclude, sexually dimorphic gene expression is common, but highly tissue-dependent. Sexually dimorphic gene expression may provide insights into mechanisms underlying phenotypic sex differences. Online data are provided as a resource for further analyses (GEO reference GSE63362). Public Library of Science 2014-12-30 /pmc/articles/PMC4280129/ /pubmed/25548914 http://dx.doi.org/10.1371/journal.pone.0115792 Text en © 2014 Huby et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Huby, Russell D. J. Glaves, Philip Jackson, Richard The Incidence of Sexually Dimorphic Gene Expression Varies Greatly between Tissues in the Rat |
title | The Incidence of Sexually Dimorphic Gene Expression Varies Greatly between Tissues in the Rat |
title_full | The Incidence of Sexually Dimorphic Gene Expression Varies Greatly between Tissues in the Rat |
title_fullStr | The Incidence of Sexually Dimorphic Gene Expression Varies Greatly between Tissues in the Rat |
title_full_unstemmed | The Incidence of Sexually Dimorphic Gene Expression Varies Greatly between Tissues in the Rat |
title_short | The Incidence of Sexually Dimorphic Gene Expression Varies Greatly between Tissues in the Rat |
title_sort | incidence of sexually dimorphic gene expression varies greatly between tissues in the rat |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4280129/ https://www.ncbi.nlm.nih.gov/pubmed/25548914 http://dx.doi.org/10.1371/journal.pone.0115792 |
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