Universal Stem-Loop Primer Method for Screening and Quantification of MicroRNA

RT-qPCR is the accepted technique for the quantification of microRNA (miR) expression: however, stem-loop RT-PCR, the most frequently used method for quantification of miRs, is time- and reagent-consuming as well as inconvenient for scanning. We established a new method called ‘universal stem-loop p...

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Detalles Bibliográficos
Autores principales: Yang, Li-hong, Wang, Si-lu, Tang, Li-li, Liu, Biao, Ye, Wen-le, Wang, Ling-ling, Wang, Zhang-yang, Zhou, Meng-tao, Chen, Bi-cheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4280144/
https://www.ncbi.nlm.nih.gov/pubmed/25548906
http://dx.doi.org/10.1371/journal.pone.0115293

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